Dual-label STED nanoscopy of living cells using photochromism

Nano Letters

Volumn 11, Issue 9, 2011, Pages 3970-3973

  Willig, Katrin I ^a   Stiel, Andre C ^a   Brakemann, Tanja ^a   Jakobs, Stefan ^a   Hell, Stefan W ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

green fluorescent protein; Microscopy; photoswitching; RSFP; superresolution; two color

Indexed keywords

GREEN FLUORESCENT PROTEIN; PHOTOSWITCHING; RSFP; SUPER RESOLUTION; TWO-COLOR;

CELLS; FLUORESCENCE; MAMMALS; MICROSCOPIC EXAMINATION; PHOTOCHROMISM; STIMULATED EMISSION;

PROTEINS;

FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN;

ANIMAL; ARTICLE; CERCOPITHECUS; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; LIGHT; METABOLISM; METHODOLOGY; NANOTECHNOLOGY; PHOTOCHEMISTRY; VERO CELL;

ANIMALS; CERCOPITHECUS AETHIOPS; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; LIGHT; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NANOTECHNOLOGY; PHOTOCHEMISTRY; VERO CELLS;

EID: 80052801182     PISSN: 15306984     EISSN: 15306992     Source Type: Journal    
DOI: 10.1021/nl202290w     Document Type: Article

References (17)

1. Hell, S. W. Microscopy and its focal switch Nat. Methods 2009, 6 (1) 24-32
2. Hell, S. W.; Wichmann, J. Breaking the diffraction resolution limit by stimulated-emission-stimulated-emission-depletion fluorescence microscopy Opt. Lett. 1994, 19 (11) 780-782
3. Klar, T. A.; Jakobs, S.; Dyba, M.; Egner, A.; Hell, S. W. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 8206-8210 (Pubitemid 30639649)
4. Westphal, V.; Rizzoli, S. O.; Lauterbach, M. A.; Kamin, D.; Jahn, R.; Hell, S. W. Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement Science 2008, 320 (5873) 246-249 (Pubitemid 351521423)
5. Hein, B.; Willig, K. I.; Wurm, C. A.; Westphal, V.; Jakobs, S.; Hell, S. W. Stimulated Emission Depletion Nanoscopy of Living Cells Using SNAP-Tag Fusion Proteins Biophys. J. 2010, 98, 158-163
6. Fitzpatrick, J. A. J.; Yan, Q.; Sieber, J. J.; Dyba, M.; Schwarz, U.; Szent-Gyorgyi, C.; Woolford, C. A.; Berget, P. B.; Waggoner, A. S.; Bruchez, M. P. STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins Bioconjugate Chem. 2009, 20 (10) 1843-1847
7. Nägerl, U. V.; Willig, K. I.; Hein, B.; Hell, S. W.; Bonhoeffer, T. Live-cell imaging of dendritic spines by STED microscopy Proc. Natl. Acad. Sci. U.S.A. 2008, 105 (48) 18982-18987
8. Donnert, G.; Keller, J.; Wurm, C. A.; Rizzoli, S. O.; Westphal, V.; Schoenle, A.; Jahn, R.; Jakobs, S.; Eggeling, C.; Hell, S. W. Two-Color Far-Field Fluorescence Nanoscopy Biophys. J. 2007, 92 (8) L67-L69
9. Neumann, D.; Bückers, J.; Kastrup, L.; Hell, S.; Jakobs, S. Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms PMC Biophys. 2010, 5 (3) 1-4
10. Schmidt, R.; Wurm, C. A.; Jakobs, S.; Engelhardt, J.; Egner, A.; Hell, S. W. Spherical nanosized focal spot unravels the interior of cells Nat. Methods 2008, 5 (6) 539-544 (Pubitemid 351761762)
11. Gugel, H.; Giske, A.; Dyba, M.; Sieber, J., Confocal imaging at the nanoscale with two-color STED microscopy. Proc. SPIE 2011, 7905.
12. Friedemann, K.; Turshatov, A.; Landfester, K.; Crespy, D. Characterization via Two-Color STED Microscopy of Nanostructured Materials Synthesized by Colloid Electrospinning Langmuir 2011, 27 (11) 7132-7139
13. Bückers, J.; Wildanger, D.; Vicidomini, G.; Kastrup, L.; Hell, S. W. Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses Opt. Express 2011, 19 (4) 3130-3143
14. Andresen, M.; Stiel, A. C.; Folling, J.; Wenzel, D.; Schonle, A.; Egner, A.; Eggeling, C.; Hell, S. W.; Jakobs, S. Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy Nat. Biotechnol. 2008, 26 (9) 1035-1040
15. Stiel, A. C.; Trowitzsch, S.; Weber, G.; Andresen, M.; Eggeling, C.; Hell, S. W.; Jakobs, S.; Wahl, M. C. 1.8 angstrom bright-state structure of the reversibly switchable fluorescent protein dronpa guides the generation of fast switching variants Biochem. J. 2007, 402 (1) 35-42
16. Ando, R.; Mizuno, H.; Miyawaki, A. Regulated Fast Nucleocytoplasmic Shuttling Observed by Reversible Protein Highlighting Science 2004, 306 (5700) 1370-1373 (Pubitemid 39532513)
17. Leutenegger, M.; Eggeling, C.; Hell, S. W. Analytical description of STED microscopy performance Opt. Express 2010, 18 (25) 26417-26429