High molecular weight glutenin subunits in some durum wheat cultivars investigated by means of mass spectrometric techniques

Journal of Agricultural and Food Chemistry

Volumn 59, Issue 22, 2011, Pages 12226-12237

  Muccilli, Vera ^a   Lo Bianco, Marisol ^b   Cunsolo, Vincenzo ^a   Saletti, Rosaria ^a   Gallo, Giulia ^b   Foti, Salvatore ^a  

^a UNIVERSITY OF CATANIA   (Italy)

^b Stazione Consorziale Sperimentale di Granicoltura per la Sicilia   (Italy)

Author keywords

gluten proteins; mass spectrometry; sequence determination; Timilia; Triticum durum

Indexed keywords

ACIDIC SEQUENCES; AMINO ACID SUBSTITUTION; DURUM WHEAT CULTIVARS; DURUM WHEATS; GLUTEN PROTEIN; HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNITS; INTACT PROTEINS; IONIZATION MASS SPECTROMETRY; MALDI-MASS SPECTROMETRY; MASS MAPPING; MASS SPECTRA; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; PRIMARY STRUCTURES; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; RP-HPLC; SEQUENCE ALIGNMENTS; SEQUENCE DETERMINATION; SEQUENCE SIMILARITY; SPECTROMETRIC TECHNIQUES; TIMILIA; TRITICUM; TRYPTIC DIGESTION; TRYPTIC PEPTIDES;

AMINO ACIDS; CULTIVATION; DESORPTION; IONIZATION OF LIQUIDS; MASS SPECTROMETERS; MASS SPECTROMETRY; PEPTIDES;

MOLECULAR WEIGHT;

GLUTEN; GLUTENIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; EVALUATION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR GENETICS; MOLECULAR WEIGHT; PROTEIN SUBUNIT; SEQUENCE ALIGNMENT; WHEAT;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLUTENS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PLANT PROTEINS; PROTEIN SUBUNITS; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRITICUM;

TRITICUM TURGIDUM SUBSP. DURUM;

EID: 81255128917     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf203139s     Document Type: Article

References (26)

1. Shewry, P. R.; Field, J. M.; Faulks, A. J.; Parmar, S.; Miflin, B. J.; Dietler, M. D.; Lew, E. J. L.; Kasarda, D. D. The purification and N-terminal amino acid sequence analysis of the high molecular weight gluten polypeptides of wheat Biochim. Biophys. Acta 1984, 788, 23-34
2. Woychik, J. H.; Boundy, J. A.; Dimler, R. J. Starch gel electrophoresis of wheat gluten proteins with concentrated urea Arch. Biochem. Biophys. 1961, 84, 477-482
3. Shewry, P. R.; Tatham, A. S. The prolamin storage proteins of cereal seeds: structure and evolution Biochem. J. 1990, 267, 17-22
4. Shewry, P. R.; Halford, N. G.; Tatham, A. S. High molecular weight subunits of wheat glutenin J. Cereal Sci. 1992, 15, 105-120
5. Waines, J. G.; Payne, P. I. Electrophoretic analysis of the highmolecular-weight glutenin subunits of Triticum monococcum, T. urartu, and the A genome of bread wheat (T. aestivum) Theor. Appl. Genet. 1987, 74, 71-76
6. r glutenin subunit found in Swedish wheats J. Cereal Sci. 1993, 17, 237-245
7. Margiotta, B.; Urbano, M.; Colaprico, G.; Johansson, E.; Buonocore, F.; DOvidio, R.; Lafiandra, D. Detection of y-type subunit at the Glu-A1 locus in some Swedish bread wheat lines J. Cereal Sci. 1996, 23, 203-211 (Pubitemid 126358372)
8. Payne, P. I.; Lawrence, G. D. Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1 and Glu-D1 which code for high molecular weight subunits of glutenin in hexaploid wheat Cereal Res. Commun. 1983, 11, 29-35
9. Payne, P. I.; Corfield, K. G.; Holt, L. M.; Blackman, J. A. Correlations between the inheritance of certain high molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat J. Sci. Food Agric. 1981, 32, 51-60
10. Payne, P. I.; Nightingale, M. A.; Krattiger, A. F.; Holt, L. M. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties J. Sci. Food Agric. 1987, 40, 51-65
11. Dworschak, R. G.; Ens, W.; Standing, K. G.; Preston, K. R.; Marchylo, B. A.; Nightingale, M. J.; Stevenson, S. G.; Hatcher, D. W. Analysis of wheat gluten proteins by matrix-assisted laser desorption/ionisation mass spectrometry J. Mass Spectrom. 1998, 33, 429-435 (Pubitemid 28222502)
12. Foti, S.; Maccarrone, G.; Saletti, R.; Roepstorff, P.; Gilbert, S.; Tatham, A. S.; Shewry, P. R. Verification of the cDNA deduced sequence of glutenin subunit 1Dx5 and an Mr 58000 repetitive peptide by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) J. Cereal Sci. 2000, 31, 173-183 (Pubitemid 30648034)
13. Cozzolino, R.; Di Giorgi, S.; Fisichella, S.; Garozzo, D.; Lafiandra, D.; Palermo, A. Matrix-assisted laser desorption/ionization mass spectrometric peptide mapping of high molecular weight glutenin subunits 1Bx7 and 1Dy10 in Cheyenne cultivar Rapid Commun. Mass Spectrom. 2001, 15, 778-787 (Pubitemid 32479840)
14. Cozzolino, R.; Di Giorgi, S.; Fisichella, S.; Garozzo, D.; Lafiandra, D.; Palermo, A.. Proteomics of gluten: mapping of subunit 1Ax2* in Cheyenne cultivar by matrix-assisted laser desorption ionization Rapid Commun. Mass Spectrom. 2001, 15, 1129-1135 (Pubitemid 32674832)
15. Cunsolo, V.; Foti, S.; Saletti, R.; Gilbert, S.; Tatham, A. S.; Shewry, P. R. Investigation and correction of the gene-derived sequence of glutenin subunit 1Dx2 by matrix-assisted laser desorption/ionisation mass spectrometry Rapid Commun. Mass Spectrom. 2002, 16, 1911-1918
16. Cunsolo, V.; Foti, S.; Saletti, R.; Gilbert, S.; Tatham, A. S.; Shewry, P. R. Structural studies of glutenin subunits 1Dy10 and 1Dy12 by matrix-assisted laser desorption/ionisation mass spectrometry and high-performance liquid chromatography/electrospray ionisation mass spectrometry Rapid Commun. Mass Spectrom. 2003, 17, 442-454 (Pubitemid 36267980)
17. Cunsolo, V.; Foti, S.; Saletti, R.; Gilbert, S.; Tatham, A. S.; Shewry, P. R. Structural studies of the allelic wheat glutenin subunits 1Bx7 and 1Bx20 by matrix-assisted laser desorption/ionisation mass spectrometry and high-performance liquid chromatography/electrospray ionisation mass spectrometry J. Mass Spectrom 2004, 39, 66-78 (Pubitemid 38208533)
18. Cunsolo, V.; Foti, S.; Saletti, R. Mass spectrometry in the characterization of cereal seed proteins Eur. J. Mass Spectrom. 2004, 10, 359-370 (Pubitemid 38887058)
19. Boggini, G. Lattitudine panificatoria del grano duro Tecnica Molitoria 2009, 60, 166-176
20. Boggini, G.; Palumbo, M.; Calcagno, F. Characterization and utilization of sicilian landraces of durum wheat in breeding programmes. In Wheat Genetic Resources: Meeting Diverse Needs; Srivastava, J. P.,; Damania, A. B., Eds.; John Wiley and Sons: Chichester, U.K., 1990; pp 223-234.
21. Marchylo, B. A.; Kruger, J. E.; Hatcher, D. W. Quantitative reversed-phase high-performance liquid chromatographic analysis of wheat storage proteins as a potential quality prediction tool J. Cereal Sci. 1989, 9, 113-130
22. Kussmann, M.; Nordhoff, E.; Rahbek-Nielsen, H.; Haebel, S.; Martin, R. L.; Jakobsen, L.; Gobom, J.; Mirgorodskaya, E.; Kroll-Kristensen, A.; Palm, L.; Roepstorff, P. Matrix-assisted laser desorption/ionization mass spectrometry sample preparation techniques designed for various peptide and protein analytes J. Mass Spectrom. 1997, 32, 593-601 (Pubitemid 27273949)
23. Gobom, J.; Nordhoff, E.; Mirgorodskaya, E.; Ekman, R.; Roepstorff, P. Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ionization mass spectrometry J. Mass Spectrom. 1999, 34, 105-116
24. Raciti, C. N.; Doust, M. A.; Lombardo, G. M.; Boggini, G.; Pecetti, L. Characterization of durum wheat mediterranean germplasm for high and low molecular weight glutenin subunits in relation with quality Eur. J. Agron. 2003, 19, 373-382 (Pubitemid 36770455)
25. Kong, X. Y.; Gu, Y. Q.; You, F. M.; Dubcovsky, J.; Anderson, O. D. Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat Plant Mol. Biol. 2004, 54, 55-69 (Pubitemid 39001406)
26. Sgrulletta, D.; Russo, G.; Casale, C.; Conciatori, A.; De Stefanis, E.; Nocente, F. Characterization of typical whole-grain breads made in Sicily (Italy) Tecnica Molitoria Int. 2010, 61, 87-93