Rapid colorimetric testing for pyrazinamide susceptibility of M. tuberculosis by a PCR-Based In-Vitro synthesized Pyrazinamidase method

PLoS ONE

Volumn 6, Issue 11, 2011, Pages

  Zhou, Man ^a,c   Geng, Xuelei ^a,c   Chen, Jun ^b   Wang, Xude ^a   Wang, Dianbing ^a   Deng, Jiaoyu ^a   Zhang, Zhiping ^a   Wang, Weihua ^b   Zhang, Xian En ^a   Wei, Hongping ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b Wuan Tuberculosis Control Center   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; PYRAZINAMIDASE; PYRAZINAMIDE; UNCLASSIFIED DRUG; AMIDASE; PYRAZINAMIDE DEAMIDASE; TUBERCULOSTATIC AGENT;

5' UNTRANSLATED REGION; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; COLORIMETRY; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; GENE; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; IN VITRO STUDY; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PNCA GENE; POLYMERASE CHAIN REACTION; SPECTROMETER; ANTIBIOTIC RESISTANCE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MUTATION; TUBERCULOSIS;

MYCOBACTERIUM TUBERCULOSIS; TRITICUM AESTIVUM;

AMIDOHYDROLASES; ANTITUBERCULAR AGENTS; COLORIMETRY; DNA, BACTERIAL; DRUG RESISTANCE, MICROBIAL; HUMANS; MICROBIAL SENSITIVITY TESTS; MUTATION; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; PYRAZINAMIDE; TUBERCULOSIS;

EID: 80755152359     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0027654     Document Type: Article

References (28)

1. Heifets LB, Cangelosi GA, (1999) Drug susceptibility testing of Mycobacterium tuberculosis: a neglected problem at the turn of the century. Int J Tuberc Lung Dis 3: 564-581.
2. Gonzalez-Martin J, Garcia-Garcia JM, Anibarro L, Vidal R, Esteban J, et al. (2010) Consensus Document on the Diagnosis, Treatment and Prevention of Tuberculosis. Arch Bronconeumol 46: 255-274.
3. Leung CC, Rieder HL, Lange C, Yew WW, (2011) Treatment of latent infection with Mycobacterium tuberculosis: update 2010. Eur Respir J 37: 690-711.
4. Butler WR, Kilburn JO, (1983) Susceptibility of Mycobacterium tuberculosis to pyrazinamide and its relationship to pyrazinamidase activity. Antimicrob Agents Chemother 24: 600-601.
5. Heifets L, (2002) Susceptibility testing of Mycobacterium tuberculosis to pyrazinamide. J Med Microbiol 51: 11-12.
6. Salfinger M, Reller LB, Demchuk B, Johnson ZT, (1989) Rapid radiometric method for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. Res Microbiol 140: 301-309.
7. Heifets L, Sanchez T, (2000) New agar medium for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide. J Clin Microbiol 38: 1498-1501.
8. Scarparo C, Ricordi P, Ruggiero G, Piccoli P, (2004) Evaluation of the fully automated BACTEC MGIT 960 system for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide, streptomycin, isoniazid, rifampin, and ethambutol and comparison with the radiometric BACTEC 460TB method. J Clin Microbiol 42: 1109-1114.
9. Parsons LM, Somoskovi A, Urbanczik R, Salfinger M, (2004) Laboratory diagnostic aspects of drug resistant tuberculosis. Frontiers in Bioscience 9: 2086-2105.
10. Hewlett D Jr, Horn DL, Alfalla C, (1995) Drug-resistant tuberculosis: inconsistent results of pyrazinamide susceptibility testing. JAMA 273: 916-917.
11. Zhang Y, Permar S, Sun Z, (2002) Conditions that may affect the results of susceptibility testing of Mycobacterium tuberculosis to pyrazinamide. J Med Microbiol 51: 42-49.
12. Miller MA, Thibert L, Desjardins F, Siddiqi SH, Dascal A, (1996) Growth inhibition of Mycobacterium tuberculosis by polyoxyethylene stearate present in the BACTEC pyrazinamide susceptibility test. J Clin Microbiol 34: 84-86.
13. Miller MA, Thibert L, Desjardins F, Siddiqi SH, Dascal A, (1995) Testing of susceptibility of Mycobacterium tuberculosis to pyrazinamide: comparison of Bactec method with pyrazinamidase assay. J Clin Microbiol 33: 2468-2470.
14. Dormandy J, Somoskovi A, Kreiswirth BN, Driscoll JR, Ashkin D, et al. (2007) Discrepant results between pyrazinamide susceptibility testing by the reference BACTEC 460TB method and pncA DNA sequencing in patients infected with multidrug-resistant W-Beijing Mycobacterium tuberculosis strains. Chest 131: 497-501.
15. Somoskovi A, Dormandy J, Parsons LM, Kaswa M, Goh KS, et al. (2007) Sequencing of the pncA gene in members of the Mycobacterium tuberculosis complex has important diagnostic applications: Identification of a species-specific pncA mutation in "Mycobacterium canettii" and the reliable and rapid predictor of pyrazinamide resistance. J Clin Microbiol 45: 595-599.
16. Scorpio A, Zhang Y, (1996) Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus. Nat Med 2: 662-667.
17. Jureen P, Werngren J, Toro JC, Hoffner S, (2008) Pyrazinamide resistance and pncA gene mutations in Mycobacterium tuberculosis. Antimicrob Agents Chemother 52: 1852-1854.
18. Cheng SJ, Thibert L, Sanchez T, Heifets L, Zhang Y, (2000) pncA mutations as a major mechanism of pyrazinamide resistance in Mycobacterium tuberculosis: spread of a monoresistant strain in Quebec, Canada. Antimicrob Agents Chemother 44: 528-532.
19. Hirano K, Takahashi M, Kazumi Y, Fukasawa Y, Abe C, (1998) Mutation in pncA is a major mechanism of pyrazinamide resistance in Mycobacterium tuberculosis. Tubercle and Lung Disease 78: 117-122.
20. Zhang H, Bi LJ, Li CY, Sun ZG, Deng JY, et al. (2009) Mutations found in the pncA gene of Mycobacterium tuberculosis in clinical pyrazinamide-resistant isolates from a local region of China. J Int Med Res 37: 1430-1435.
21. Lee KW, Lee JM, Jung KS, (2001) Characterization of pncA mutations of pyrazinamide-resistant Mycobacterium tuberculosis in Korea. J Korean Med Sci 16: 537-543.
22. Denkin S, Volokhov D, Chizhikov V, Zhang Y, (2005) Microarray-based pncA genotyping of pyrazinamide-resistant strains of Mycobacterium tuberculosis. J Med Microbiol 54: 1127-1131.
23. Suzuki Y, Suzuki A, Tamaru A, Katsukawa C, Oda H, (2002) Rapid detection of pyrazinamide-resistant Mycobacterium tuberculosis by a PCR-based in vitro system. J Clin Microbiol 40: 501-507.
24. Akbergenov R, Zhanybekova S, Kryldakov R, Zhigailov A, Polimbetova N, et al. (2004) ARC©\1, a sequence element complementary to an internal 18S rRNA segment, enhances translation efficiency in plants when present in the leader or intercistronic region of mRNAs. Nucleic Acids Res 32: 239.
25. Tracevska T, Jansone I, Baumanis V, Nodieva A, Marga O, et al. (2004) Spectrum of pncA mutations in multidrug-resistant Mycobacterium tuberculosis isolates obtained in Latvia. Antimicrob Agents Chemother 48: 3209-3210.
26. Chedore P, Bertucci L, Wolfe J, Sharma M, Jamieson F, (2010) Potential for Erroneous Results Indicating Resistance When Using the Bactec MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Pyrazinamide. J Clin Microbiol 48: 300-301.
27. Sheen P, Ferrer P, Gilman RH, Lopez-Llano J, Fuentes P, et al. (2009) Effect of pyrazinamidase activity on pyrazinamide resistance in Mycobacterium tuberculosis. Tuberculosis (Edinb) 89: 109-113.
28. Petrella S, Gelus-Ziental N, Maudry A, Laurans C, Boudjelloul R, et al. (2011) Crystal structure of the pyrazinamidase of Mycobacterium tuberculosis: insights into natural and acquired resistance to pyrazinamide. Plos One 6: e15785.