Generation of high-expressing cells by methotrexate amplification of destabilized dihydrofolate reductase selection marker

Methods in Molecular Biology

Volumn 801, Issue , 2012, Pages 161-172

  Ng, Say Kong ^a  

^a AGENCY FOR SCIENCE   (Singapore)

Author keywords

AU rich element; CHO; Dihydrofolate reductase; Mammalian cell; Methotrexate amplification; MTX; Murine ornithine decarboxylase; PEST; Recombinant protein expression; Stable transfection

Indexed keywords

DIHYDROFOLATE REDUCTASE; METHOTREXATE; ORNITHINE DECARBOXYLASE;

ANIMAL; ARTICLE; CELL CLONE; CHO CELL; CRICETULUS; DRUG EFFECT; GENE EXPRESSION; GENE VECTOR; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; METABOLISM; METHODOLOGY; MOUSE;

ANIMALS; CHO CELLS; CLONE CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; METHOTREXATE; MICE; ORNITHINE DECARBOXYLASE; TETRAHYDROFOLATE DEHYDROGENASE; TRANSFECTION;

MAMMALIA; MURINAE;

EID: 80555156078     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-352-3_11     Document Type: Article

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