Highly efficient selection of the stable clones expressing antibody-IL-2 fusion protein by a dicistronic expression vector containing a mutant neo gene

Journal of Immunological Methods

Volumn 295, Issue 1-2, 2004, Pages 49-56

  Chen, Liyong ^a   Xie, Zhigang ^a   Teng, Yan ^b   Wang, Minggao ^a   Shi, Ming ^a   Qian, Lu ^a   Hu, Meiru ^a   Feng, Jiannan ^a   Yang, Xiao ^b   Shen, Beifen ^a   Guo, Ning ^a  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

^b BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

Antibody cytokine fusion protein; Eukaryotic cells; Hot spot; Recombinant protein expression

Indexed keywords

ANTIBIOTIC G 418; HYBRID PROTEIN; INTERLEUKIN 2; KANAMYCIN KINASE; MESSENGER RNA; NEOMYCIN; RECOMBINANT ANTIBODY;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ANTIBODY PRODUCTION; ARTICLE; CELL GROWTH; CHO CELL; EXPRESSION VECTOR; FEMALE; GENE; GENE AMPLIFICATION; GENE EXPRESSION; GENE FUNCTION; GENE MUTATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECOMBINANT GENE; RNA TRANSLATION; ANIMAL; BIOSYNTHESIS; CELL CLONE; CRICETULUS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

EUKARYOTA;

ANIMALS; CHO CELLS; CLONE CELLS; CRICETINAE; CRICETULUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENETIC VECTORS; HUMANS; INTERLEUKIN-2; KANAMYCIN KINASE; MUTAGENESIS, SITE-DIRECTED; MUTATION; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFECTION;

EID: 13844315804     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.08.013     Document Type: Article

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