Robust assessment of protein complex formation in vivo via single-molecule intensity distributions of autofluorescent proteins

Journal of Biomedical Optics

Volumn 16, Issue 7, 2011, Pages

  Meckel, Tobias ^a,b   Semrau, Stefan ^b,c   Schaaf, Marcel J M ^c   Schmidt, Thomas ^b  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

^b LEIDEN UNIVERSITY   (Netherlands)

^c LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Biophotonics; Fluoresence spectroscopy; Laser induced fluorescence; Microscopy; Signal processing; Spectroscopy cells

Indexed keywords

AUTOFLUORESCENT PROTEINS; AVERAGE NUMBERS; BIO PHOTONICS; BIOLOGICAL PROCESS; BIOPHYSICAL TECHNIQUES; CHINESE HAMSTER OVARY CELLS; CLUSTER FORMATIONS; DIFFRACTION LIMITED; ENHANCED YELLOW FLUORESCENT PROTEINS; EXPERIMENTAL CONDITIONS; FLUORESCENT PROTEIN; FLUORESENCE SPECTROSCOPY; IN-VIVO; INTENSITY DISTRIBUTION; LABELED PROTEINS; LASER-INDUCED FLUORESCENCE; LIVE CELL; LIVING CELL; MEMBRANE ANCHORS; PHOTOPHYSICAL; PHOTOPHYSICS; PROTEIN COMPLEXES; SINGLE-MOLECULE; SINGLE-MOLECULE MICROSCOPY; THEORETICAL PREDICTION;

CELL MEMBRANES; FLUORESCENCE; MOLECULES; SIGNAL PROCESSING;

PROTEINS;

CRICETULUS GRISEUS; RATTUS;

BACTERIAL PROTEIN; HRAS PROTEIN, HUMAN; HYBRID PROTEIN; MULTIPROTEIN COMPLEX; PHOTOPROTEIN; PROTEIN P21; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ANIMAL; ARTICLE; BIOLOGICAL MODEL; BIOPHYSICS; CELL MEMBRANE; CHEMISTRY; CHO CELL; CRICETULUS; FLUORESCENCE; FLUORESCENCE MICROSCOPY; HAMSTER; HUMAN; LIGHT RELATED PHENOMENA; METABOLISM; RAT; SPECTROFLUOROMETRY; TUMOR CELL LINE; VALIDATION STUDY;

ANIMALS; BACTERIAL PROTEINS; BIOPHYSICAL PHENOMENA; CELL LINE, TUMOR; CELL MEMBRANE; CHO CELLS; CRICETINAE; CRICETULUS; FLUORESCENCE; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; MULTIPROTEIN COMPLEXES; OPTICAL PHENOMENA; PROTO-ONCOGENE PROTEINS P21(RAS); RATS; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 80455141531     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.3600002     Document Type: Article

References (25)

1. N. J. Leonard, R. Ghirlando, J. Askins, J. K. Bell, D. H. Margulies, D. R. Davies, and D. M. Segal, "The TLR3 signaling complex forms by cooperative receptor dimerization," Proc. Natl. Acad. Sci. USA 105, 258-263 (2008).
2. J. Schlessinger, "Ligand-induced, receptor-mediated dimerization and activation of EGF receptor," Cell 110, 669-672 (2002). (Pubitemid 35283957)
3. P. H. M. Lommerse, B. Snaar-Jagalska, H. P. Spaink, and T. Schmidt, "Single-molecule diffusionmeasurements of H-Ras at the plasma membrane of live cells reveal microdomain localization upon activation," J. Cell Sci. 118, 1799-1809 (2005). (Pubitemid 40723825)
4. J. Gandia, C. Lluis, S. Ferre, R. Franco, and F. Ciruela, "Light resonance energy transfer-based methods in the study of G protein-coupled receptor oligomerization," BioEssays 30, 82-89 (2008).
5. T. Keppola, "Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells," Annu. Rev. Biophys. 37, 465-487 (2008).
6. M. A. Digman, R. Dalal, A. F. Horwitz, and E. Gratton, "Mapping the number of molecules and brightness in the laser scanning microscope," Biophys. J. 94, 2320-2332 (2008).
7. D.Kolin and P.Wiseman, "Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells," Cell Biochem. Biophys. 49, 141-164 (2007). (Pubitemid 350242576)
8. V. Vukojevic, M. Heidkamp, Y. Ming, R. Johansson, L. Terenius, and R. Rigler, "Quantitative single-molecule imaging by confocal laser scanning microscopy," Proc. Natl. Acad. Sci. USA 105, 18176-18181 (2008).
9. G. Malengo, A. Andolfo, N. Sidenius, E. Gratton, M. Zamai, and V. Caiolfa, "Fluorescence correlation spectroscopy and photon counting histogram on membrane proteins: functional dynamics of the glycosylphosphatidylinositol-anchored urokinase plasminogen activator receptor," J. Biomed. Opt. 13, 031215 (2008).
10. T. Schmidt, G. Schütz, H. Gruber, and H. Schindler, "Local stoichiometries determined by counting individual molecules," Anal. Chem. 68, 4397-4401 (1996).
11. T. McAnaney, W. Zeng, C. Doe, N. Bhanji, S. Wakelin, D. Pearson, P. Abbyad, X. Shi, S. Boxer, and C. Bagshaw, "Anomalous negative fluorescence anisotropy in yellow fluorescent protein (YFP 10C): quantitative analysis of FRET in YFP dimers," Biochemistry 44, 5510-5524 (2005).
12. M. H. Ulbrich and E. Y. Isacoff, "Subunit counting in membrane-bound proteins," Nat. Methods 4, 319-321 (2007). (Pubitemid 46766975)
13. L. Cognet, C. Tardin, M. L. M. Negrier, C. Breillat, F. Coussen, D. Choquet, andB. Lounis, "Robust single-molecule approach for counting autofluorescent proteins," J. Biomed. Opt. 13, 031216 (2008).
14. L. Mandel, "Fluctuations of photon beams and their correlations," Proc. Phys. Soc. 72, 1037-1048 (1958).
15. P. H. M. Lommerse, G. A. Blab, L. Cognet, G. S. Harms, B. E. Snaar-Jagalska, H. P. Spaink, and T. Schmidt, "Single-molecule imaging of the H-Ras membrane-anchor reveals domains in the cytoplasmic leaflet of the cell membrane," Biophys. J. 86, 609-616 (2004). (Pubitemid 38067490)
16. M. Ormö, A. B. Cubitt, K. Kallio, L. A. Gross, R. Y. Tsien, and S. J. Remington, "Crystal structure of the Aequorea victoria green fluorescent protein," Science 273, 1392-1392 (1996).
17. G. H. Patterson, D. W. Piston, and B. G. Barisas, "FÃ rster distances between green fluorescent protein pairs," Anal. Biochem. 284, 438-440 (2000).
18. T. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, "Imaging of single molecule diffusion," Proc. Nat. Acad. Sci. USA 93, 2926-2929 (1996). (Pubitemid 26114340)
19. P. Hinterdorfer and A. van Oijen, Eds., Handbook of Single-Molecule Biophysics, Springer New York (2009).
20. G. S. Harms, L. Cognet, P. H. M. Lommerse, G. A. Blab, and T. Schmidt, "Autofluorescent proteins in single-molecule research: applications to live cell imaging microscopy," Biophys. J. 80, 2396-2408 (2001). (Pubitemid 32402002)
21. Y. Chen, J.Mueller, P. T. C. So, and E. Gratton, "Resolving heterogeneity on the single molecular level with the photon-counting histogram," Biophys. J. 77, 553-567 (1999).
22. K. Palo, U.Mets, V. Loorits, and P. Kask, "Calculation of photon-count number distributions via master equations," Biophys. J. 90, 2179-2191 (2006).
23. A. Papoulis, Probability, Random Variables and Stochastic Processes, McGRaw-Hill, New York (1991).
24. S. Mutch,B. Fujimoto, C.Kuyper, J.Kuo, and S.Bajjalieh, "Deconvolving single-molecule intensity distributions for quantitative microscopy measurements," Biophys. J. 92, 2926-2943 (2007). (Pubitemid 46557866)
25. J. Schuster, F. Cichos, and C. von Borczyskowski, "Diffusion measurements by single-molecule spot-size analysis," J. Phys. Chem. A 106, 5403-5406 (2002). (Pubitemid 35289947)