Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption

Journal of Chromatography A

Volumn 1217, Issue 15, 2010, Pages 2262-2266

  Gomes, A Gabriela ^a   Azevedo, Ana M ^a   Aires Barros, M Raquel ^a   Prazeres, D Miguel F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Adsorption; Affinity process; Chromatography; Gene therapy; Genomic DNA; Lipopolysaccharides; Phenyl boronate; Plasmid DNA; Purification; RNA

Indexed keywords

ADSORPTION AFFINITY; AFFINITY PROCESS; BORONATE; GENOMIC DNA; LIPOPOLYSACCHARIDES; PHENYL BORONATE; PLASMID DNA;

ADSORPTION; DNA; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE THERAPY; GENETIC ENGINEERING; GLASS; HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; PROTEINS; PURIFICATION; RNA;

MICROBIOLOGY;

BORONIC ACID DERIVATIVE; GENOMIC DNA; PLASMID DNA; RNA;

ADSORPTION; ARTICLE; CELL LYSATE; CONTROLLED STUDY; COVALENT BOND; DNA BINDING; ELECTROPHORESIS; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOST CELL; HYDROPHOBICITY; INTERMETHOD COMPARISON; MATERIAL STATE; NONHUMAN; PH; PRIORITY JOURNAL;

ADSORPTION; BORONIC ACIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, AGAR GEL; ESCHERICHIA COLI; MACROMOLECULAR SUBSTANCES; NUCLEIC ACIDS; PLASMIDS; SOLUTIONS;

ESCHERICHIA COLI;

EID: 77950690475     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.02.015     Document Type: Article

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