Quantification of γH2AX foci in response to Ionising radiation

Journal of Visualized Experiments

Volumn , Issue 38, 2010, Pages

  Mah, Li Jeen ^a,b   Vasireddy, Raja S ^a,b   Tang, Michelle M ^a   Georgiadis, George T ^a   El Osta, Assam ^a,b   Karagiannis, Tom C ^a,b  

^a BAKER IDI HEART AND DIABETES INSTITUTE   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Chromatin modification; DNA damage; DNA double strand break; H2AX; Ionising radiation; Issue 38; Medicine; Repair

Indexed keywords

EID: 80055109176     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1957     Document Type: Article

References (10)

1. Rogakou, E.P., Boon, C., Redon, C., & Bonner, W.M., Megabase chromatin domains involved in DNA double-strand breaks in vivo. J. Cell Biol. 146, 905-916 (1999).
2. Bonner, W.M. et al., Gamma H2AX and cancer. Nature Reviews Cancer 8 (12), 957-967 (2008).
3. Savic, V. et al., Formation of Dynamic [gamma]-H2AX Domains along Broken DNA Strands Is Distinctly Regulated by ATM and MDC1 and Dependent upon H2AX Densities in Chromatin. Molecular Cell 34 (3), 298-310 (2009).
4. Downs, J. et al., Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites. Mol Cell. 16 (6), 979-990 (2004).
5. Chowdhury, D. et al., [gamma]-H2AX Dephosphorylation by Protein Phosphatase 2A Facilitates DNA Double-Strand Break Repair. Molecular Cell 20 (5), 801-809 (2005).
6. Nakada, S., Chen, G., Gingras, A., & Durocher, D., PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint. EMBO Rep. 9 (10), 1019-1026 (2008).
7. Altaf, M., Auger, A., Covic, M., & Côté, J., Connection between histone H2A variants and chromatin remodeling complexes. Biochem Cell Biol. 87 (1), 35-50 (2009).
8. Kusch, T. et al., Acetylation by Tip60 Is Required for Selective Histone Variant Exchange at DNA Lesions. Science 306 (5704), 2084-2087 (2004).
9. Hurlin, P. et al., Progression of human papillomavirus type 18-immortalized human keratinocytes to a malignant phenotype. Proc Natl Acad Sci U S A 88 (2), 570-574 (1991).
10. Dickey, J. et al., H2AX: functional roles and potential applications. Chromosoma [Epub ahead of print] (2009).