Subunit stoichiometry of human Orai1 and Orai3 channels in closed and open states

Proceedings of the National Academy of Sciences of the United States of America

Volumn 108, Issue 43, 2011, Pages 17832-17837

  Demuro, Angelo ^a   Penna, Aubin ^a   Safrina, Olga ^a   Yeromin, Andriy V ^a   Amcheslavsky, Anna ^a   Cahalan, Michael D ^a   Parker, Ian ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Ca 2+ release activated ca 2+; Calcium signaling

Indexed keywords

CALCIUM CHANNEL; CALCIUM CHANNEL ORAI3; CALCIUM ION; CALCIUM RELEASE ACTIVATED CALCIUM CHANNEL 1; DIMER; STROMAL INTERACTION MOLECULE 1; TETRAMER; UNCLASSIFIED DRUG;

ARTICLE; BLEACHING; CALCIUM CELL LEVEL; CALCIUM CONDUCTANCE; CALCIUM SIGNALING; CALCIUM TRANSPORT; CELL MEMBRANE; CELLULAR DISTRIBUTION; CHANNEL GATING; CONTROLLED STUDY; CYTOSOL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; STOICHIOMETRY; SUBUNIT STOICHIOMETRY; WILD TYPE;

BLOTTING, WESTERN; BORON COMPOUNDS; CALCIUM CHANNELS; CALCIUM SIGNALING; CELL LINE; FLUORESCENT DYES; FURA-2; GREEN FLUORESCENT PROTEINS; HUMANS; PATCH-CLAMP TECHNIQUES; PHOTOBLEACHING; POLYMERS; PROTEIN SUBUNITS;

MAMMALIA;

EID: 80055076956     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1114814108     Document Type: Article

References (40)

1. Parekh AB, Putney JW, Jr. (2005) Store-operated calcium channels. Physiol Rev 85:757-810. (Pubitemid 40404641)
2. 2+ entry. Nat Cell Biol 11:669-677.
3. Feske S, et al. (2006) A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 441:179-185.
4. 2+ influx. Curr Biol 15:1235-1241.
5. Roos J, et al. (2005) STIM1, an essential and conserved component of store-operated Ca2+ channel function. J Cell Biol 169:435-445. (Pubitemid 40686694)
6. Vig M, et al. (2006) CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science 312:1220-1223. (Pubitemid 43801149)
7. 2+ channel activity. Proc Natl Acad Sci USA 103:9357-9362.
8. Cahalan MD, Chandy KG (2009) The functional network of ion channels in T lymphocytes. Immunol Rev 231:59-87.
9. Huang GN, et al. (2006) STIM1 carboxyl-terminus activates native SOC, I(crac) and TRPC1 channels. Nat Cell Biol 8:1003-1010. (Pubitemid 44314732)
10. Ji W, et al. (2008) Functional stoichiometry of the unitary calcium-release-activated calcium channel. Proc Natl Acad Sci USA 105:13668-13673.
11. Muik M, et al. (2008) Dynamic coupling of the putative coiled-coil domain of ORAI1 with STIM1 mediates ORAI1 channel activation. J Biol Chem 283:8014-8022.
12. Penna A, et al. (2008) The CRAC channel consists of a tetramer formed by Stim-induced dimerization of Orai dimers. Nature 456:116-120.
13. Yuan JP, Zeng W, Huang GN, Worley PF, Muallem S (2007) STIM1 heteromultimerizes TRPC channels to determine their function as store-operated channels. Nat Cell Biol 9:636-645. (Pubitemid 47214725)
14. 2+ channel activity of human Orai1 and Orai3. J Biol Chem 283:17662-17671.
15. Kawasaki T, Lange I, Feske S (2009) A minimal regulatory domain in the C terminus of STIM1 binds to and activates ORAI1 CRAC channels. Biochem Biophys Res Commun 385:49-54.
16. Muik M, et al. (2009) A cytosolic homomerization and a modulatory domain within STIM1 C terminus determine coupling to ORAI1 channels. J Biol Chem 284:8421-8426.
17. Park CY, et al. (2009) STIM1 clusters and activates CRAC channels via direct binding of a cytosolic domain to Orai1. Cell 136:876-890.
18. Yuan JP, et al. (2009) SOAR and the polybasic STIM1 domains gate and regulate Orai channels. Nat Cell Biol 11:337-343.
19. Zhou Y, et al. (2010) STIM1 gates the store-operated calcium channel ORAI1 in vitro. Nat Struct Mol Biol 17:112-116.
20. Mignen O, Thompson JL, Shuttleworth TJ (2008) Orai1 subunit stoichiometry of the mammalian CRAC channel pore. J Physiol 586:419-425.
21. 2+ channel function in the heterozygous condition. J Biol Chem 284:6620-6626.
22. Gwack Y, et al. (2007) Biochemical and functional characterization of Orai proteins. J Biol Chem 282:16232-16243. (Pubitemid 47100386)
23. Madl J, et al. (2010) Resting state Orai1 diffuses as homotetramer in the plasma membrane of live mammalian cells. J Biol Chem 285:41135-41142.
24. Ulbrich MH, Isacoff EY (2007) Subunit counting in membrane-bound proteins. Nat Methods 4:319-321. (Pubitemid 46766975)
25. 3 receptors. J Physiol 536:3-19.
26. Yeromin AV, Roos J, Stauderman KA, Cahalan MD (2004) A store-operated calcium channel in Drosophila S2 cells. J Gen Physiol 123:167-182. (Pubitemid 38176608)
27. DeHaven WI, Smyth JT, Boyles RR, Bird GS, Putney JW, Jr. (2008) Complex actions of 2-aminoethyldiphenyl borate on store-operated calcium entry. J Biol Chem 283:19265-19273.
28. Peinelt C, Lis A, Beck A, Fleig A, Penner R (2008) 2-Aminoethoxydiphenyl borate directly facilitates and indirectly inhibits STIM1-dependent gating of CRAC channels. J Physiol 586:3061-3073. (Pubitemid 351984690)
29. 2+ stores. Proc Natl Acad Sci USA 90:6295-6299.
30. Mercer JC, et al. (2006) Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J Biol Chem 281:24979-24990. (Pubitemid 44274268)
31. Peinelt C, et al. (2006) Amplification of CRAC current by STIM1 and CRACM1 (Orai1). Nat Cell Biol 8:771-773.
32. Ding H, Wong PT, Lee EL, Gafni A, Steel DG (2009) Determination of the oligomer size of amyloidogenic protein beta-amyloid(1-40) by single-molecule spectroscopy. Biophys J 97:912-921.
33. 2+ influx and modulates gene expression. Nat Chem Biol 7:351-358.
34. Tombola F, Ulbrich MH, Isacoff EY (2008) The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor. Neuron 58:546-556. (Pubitemid 351672357)
35. Xu P, et al. (2006) Aggregation of STIM1 underneath the plasma membrane induces clustering of Orai1. Biochem Biophys Res Commun 350:969-976. (Pubitemid 44584187)
36. 2+ entry: Local activation of CRAC channels by STIM1 at ER-plasma membrane junctions. J Cell Biol 174:815-825.
37. Li Z, et al. (2011) Graded activation of CRAC channel by binding of different numbers of STIM1 to Orai1 subunits. Cell Res 21:305-315.
38. 2+ (CRAC) channels by stromal interaction molecule 1 (STIM1). Proc Natl Acad Sci USA 108:13299-13304.
39. 2+ clearance distinguish Th1 and Th2 lymphocytes. J Immunol 164:1153-1160.
40. 2+ flux through individual muscle acetylcholine receptor channels. J Gen Physiol 126:179-192.