On-line monitoring of recombinant bacterial cultures using multi-wavelength fluorescence spectroscopy

Biochemical Engineering Journal

Volumn 58-59, Issue 1, 2011, Pages 133-139

  Jain, Garima ^a,c   Jayaraman, Guhan ^a   Kökpinar, Öznur ^b,c   Rinas, Ursula ^b,c   Hitzmann, Bernd ^d  

^a INDIAN INSTITUTE OF TECHNOLOGY MADRAS   (India)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^d UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

Chemometrics; Cultivation; Escherichia coli; Fluorescence spectroscopy; Human basic fibroblast growth factor; On line monitoring

Indexed keywords

BACTERIAL CULTURES; BASIC FIBROBLAST GROWTH FACTOR; BATCH CULTIVATION; BIOMASS CONCENTRATIONS; BIOPROCESSES; CALIBRATION DATA; CHEMOMETRIC MODELS; CHEMOMETRICS; DATA SETS; EXCITATION AND EMISSION SPECTRA; FED-BATCH CULTIVATION; GLUCOSE CONCENTRATION; HIGH GROWTH RATE; LOW GROWTH RATE; MULTIWAVELENGTH; NAD(P)H; ONLINE MONITORING; PARTIAL LEAST-SQUARES METHOD; PLS MODELS; PRODUCTION RATES; RECOMBINANT ESCHERICHIA COLI;

BIOMASS; CALIBRATION; CARBON DIOXIDE; CELL CULTURE; ECOLOGY; EMISSION SPECTROSCOPY; ESCHERICHIA COLI; FIBROBLASTS; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; GLUCOSE; GROWTH RATE; LEAST SQUARES APPROXIMATIONS; PEPTIDES; PRINCIPAL COMPONENT ANALYSIS; VOLTAGE MEASUREMENT;

FLUOROPHORES;

CARBON DIOXIDE; FIBROBLAST GROWTH FACTOR 2; GLUCOSE; QUERCETIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BIOMASS; ESCHERICHIA COLI; FED BATCH CULTURE; FLUORESCENCE SPECTROSCOPY; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 80054837888     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2011.09.005     Document Type: Article

References (19)

1. Matanguihan R.M., Konstantinov K.B., Yoshida T. Dielectric measurement to monitor the growth and the physiological states of biological cells. Bioprocess Biosyst. Eng. 1994, 11:213-222.
2. Kiviharju K., Salonen K., Moilanen U., Eerikainen T. Biomass measurement on-line: the performance of in situ measurements and software sensors. J. Ind. Microbiol. Biotechnol. 2008, 35:657-665.
3. Hantelmann K., Kollecker A., Hull D., Hitzmann B., Scheper T. Two-dimensional fluorescence spectroscopy: a novel approach for controlling fed-batch cultivations. J. Biotechnol. 2006, 121:410-417.
4. Zabriskie D.W., Humphrey A.E. Estimation of fermentation biomass concentration by measuring culture fluorescence. Appl. Environ. Microbiol. 1978, 35:337-343.
5. Ganzlin M., Marose S., Lu X., Hitzmann B., Scheper T., Rinas U. In situ multi-wavelength fluorescence spectroscopy as effective tool to simultaneously monitor spore germination, metabolic activity and quantitative protein production in recombinant Aspergillus niger fed-batch cultures. J. Biotechnol. 2007, 132:461-468.
6. Nielsen J., Johansen C.L., Villadsen J. Culture fluorescence measurements during batch and fed-batch cultivations with Penicillium chrysogenum. J. Biotechnol. 1994, 38:51-62.
7. Siano S.A., Mutharasan R. NADH and flavin fluorescence responses of starved yeast cultures to substrate additions. Biotechnol. Bioeng. 1989, 34:660-670.
8. Rhee J.I., Kang T.H., Lee K.I., Sohn O.J., Kim S.Y., Chung S.W. Application of principal component analysis and self-organizing map to the analysis of 2D fluorescence spectra and the monitoring of fermentation processes. Biotechnol. Bioprocess Eng. 2006, 11:432-441.
9. Rhee J.I., Kang T.H. On-line process monitoring and chemometric modeling with 2D fluorescence spectra obtained in recombinant E. coli fermentations. Process Biochem. 2007, 42:1124-1134.
10. Jhala E., Galilee C., Reinisch L. Principal component analyses of fluorescence changes upon growth conditions and washing of Pseudomonas aeruginosa. Appl. Opt. 2007, 46:5522-5528.
11. Johansson L., Liden G. A study of long-term effects on plasmid-containing Escherichia coli in carbon-limited chemostat using 2D-fluorescence spectrofluorimetry. Biotechnol. Prog. 2006, 22:1132-1139.
12. Skibsted E., Lindemann C., Roca C., Olsson L. On-line bioprocess monitoring with a multi-wavelength fluorescence sensor using multivariate calibration. J. Biotechnol. 2001, 88:47-57.
13. Boehl D., Solle D., Hitzmann B., Scheper T. Chemometric modelling with two- dimensional fluorescence data for Claviceps purpurea bioprocess characterization. J. Biotechnol. 2003, 105:179-188.
14. Surribas A., Geissler D., Gierse A., Scheper T., Hitzmann B., Montesinos J.L., Valero F.F. State variables monitoring by in situ multi-wavelength fluorescence spectroscopy in heterologous protein production by Pichia pastoris. J. Biotechnol. 2006, 124:412-419.
15. Surribas A., Montesinos J.L., Valero F.F. Biomass estimation using fluorescence measurements in Pichia pastoris bioprocess. J. Chem. Technol. Biotechnol. 2006, 81:23-28.
16. Lantz A.E., Jorgensen P., Poulsen E., Lindemann C., Olsson L. Determination of cell mass and polymyxin using multi-wavelength fluorescence. J. Biotechnol. 2006, 121:544-554.
17. Hoffmann F., van den Heuvel J., Zidek N., Rinas U. Minimizing inclusion body formation during recombinant protein production in Escherichia coli at bench and pilot plant scale. Enzyme Microb. Technol. 2004, 34:235-241.
18. Seeger A., Schneppe B., McCarthy J.E.G., Deckwer W.D., Rinas U. Comparison of temperature-induced and isopropyl-beta-d-thiogalacto-pyranoside-induced synthesis of basic fibroblast growth-factor in high-cell-density cultures of recombinant Escherichia coli. Enzyme Microb. Technol. 1995, 17:947-953.
19. Lindemann C., Marose S., Nielsen H.O., Scheper T. 2-Dimensional fluorescence spectroscopy for on-line bioprocess monitoring. Sens. Actuators B: Chem. 1998, 51:273-277.