Biomass estimation using fluorescence measurements in Pichia pastoris bioprocess

Journal of Chemical Technology and Biotechnology

Volumn 81, Issue 1, 2006, Pages 23-28

  Surribas, Anna ^a   Montesinos, José Luis ^a   Valero, Francisco F ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Biomass estimation; Bioprocess monitoring; Fluorometry; Modelling; Pichia pastoris

Indexed keywords

FLUORESCENCE; INTEGRAL EQUATIONS; MATHEMATICAL MODELS; PROTEINS; QUENCHING; YEAST;

BIOMASS CONCENTRATION; BIOMASS MONTORING; BIOPROCESS; FLUOROMETRY; HETEROLOGOUS PROTEINS; MEAN ABSOLUTE RELATIVE PREDICTION ERROR (MARE); PICHIA PASTORIS; TRYPTOPHAN FLUORESCENCE;

BIOMASS;

GLYCEROL; METHANOL; TRYPTOPHAN;

BIOMASS; FLUORESCENCE; INTEGRAL EQUATIONS; MATHEMATICAL MODELS; PROTEINS; QUENCHING; YEAST;

ARTICLE; BIOMASS; BIOPROCESS; CELL DENSITY; CONCENTRATION RESPONSE; DILUTION; FLUORESCENCE; FLUOROMETRY; FUNGAL STRAIN; MONITORING; NONHUMAN; PICHIA PASTORIS; PREDICTION;

PICHIA PASTORIS;

EID: 31444451686     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.1352     Document Type: Article

References (22)

1. Schügerl K, Progress in monitoring and control of bioprocesses during the last 20 years. J Biotechnol 86:149-173 (2001).
2. Mulchandani A and Bassi AS, Principles and applications of biosensors for bioprocess monitoring and control. Crit Rev Biotechnol 1:105-124 (1995).
3. Olsson L and Nielsen J, On-line and in situ monitoring of biomass in submerged cultivations. Trends Biotechnol 15:517-522 (1997).
4. Konstantinov K, Chuppa S, Sajan E, Tsai Y, Yoon S and Golini F, Real-time biomass-concentration monitoring in animal-cell cultures. Trends Biotechnol 12:324-333 (1994).
5. Junker BH, Reddy J, Gbewonyo K and Greasham R, Online and in situ monitoring technology for cell density measurement in microbial and animal cell culture. Bioprocess Eng 10:195-207 (1994).
6. Sonnleitner B, On-line measurement of cell concentration. Process Control & Quality 2:97-104 (1992).
7. Skibsted E, Lindemann C, Roca R and Olsson L, On-line bioprocess monitoring with multi-wavelength fluorescence sensor using multivariate calibration. J Biotechnol 88:47-57 (2001).
8. Tartakovsky B, Sheintuch M, Hilmer JM and Scheper T, Application of scanning fluorometry for monitoring of a fermentation process. Biotechnol Progr 12:126-131 (1996).
9. Marose S, Lindemann C and Scheper T, Two-dimensional fluorescence spectroscopy: a new tool for on-line bioprocess monitoring. Biotechnol Progr 14:63-74 (1998).
10. Hagedorn A, Legge RL and Budman H, Evaluation of spectrofluorometry as a tool for estimation in fed-batch fermentations. Biotechnol Bioeng 83:104-111 (2003).
11. Hagedorn A, Levadoux W, Groleau D and Tartakovsky B, Evaluation of multiwavelength culture fluorescence for monitoring the aroma compound 4-hydroxy-2 (or 5) - ethyl-5 (or 2) -methyl-(2H)-furanone (HEMF) production. Biotechnol Progr 20:361-367 (2004).
12. Scheper T, Gebauer A, Sauerbrei A, Niehoff A and Schügerl K, Measurement of biological parameters during fermentation processes. Anal Chim Acta 163:111-118 (1984).
13. DeLisa MP, Li J, Rao G, Weigand WA and Bentley WE, Monitoring GFP-Operon Fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor. Biotechnol Bioeng 65:54-64 (1999).
14. Li J, Wang S, VanDusen WJ, Schultz LD, George HA, Herber WK, Chae HJ, Bentley WE and Rao G, Green fluorescent protein in Saccharomyces cerevisiae: real-time studies of the GAL1 promoter. Biotechnol Bioeng 70:187-196 (2000).
15. Lu C, Albano R, Bentley WE and Rao G, Differential rates of gene expression monitored by green fluorescent protein. Biotechnol Bioeng 79:429-437 (2002).
16. Sarramegna V, Talmont F, Seree de Roch M, Milon A and Demange P, Green fluorescent protein as a reporter of human μ-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris. J Biotechnol 99:23-39 (2002).
17. Horvath JJ, Glazier SA and Spangler CJ, In situ fluorescence cell mass measurements of Saccharomyces cerevisiae using cellular tryptophan. Biotechnol Progr 9:666-670 (1993).
18. Lin Cereghino J and Cregg JM, Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45-66 (2000).
19. Minning S, Serrano A, Ferrer P, Solà C, Schmid RD and Valero F, Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol 86:59-70 (2001).
20. Li JK, Asali EC, Humphrey AE and Horvath JJ, Monitoring cell concentration and activity by multiple excitation fluorometry. Biotechnol Progr 7:21-27 (1991).
21. Sikorska E, Romaniuk A, Khmelinskii IV, Herance R, Bourdelande JL, Sikorski M and Koziol J, Characterization of edible oils using total luminescence spectroscopy. J Fluorescence 14:25-35 (2004).
22. Petkov SV and Davis RA, On-line biomass estimation using a modified oxygen utilization rate. Bioprocess Eng 15:43-45 (1996).