Development of a diphtheria toxin based antiporcine CD3 recombinant immunotoxin

Bioconjugate Chemistry

Volumn 22, Issue 10, 2011, Pages 2014-2020

  Wang, Zhirui ^a,b   Duran Struuck, Raimon ^a   Crepeau, Rebecca ^a   Matar, Abraham ^a   Hanekamp, Isabel ^a   Srinivasan, Srimathi ^a   Neville, David M ^c   Sachs, David H ^a,b   Huang, Christene A ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c Angimmune LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALS; BIOSYNTHESIS; BODY FLUIDS; CYTOLOGY; DISEASE CONTROL; HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; LIQUID CHROMATOGRAPHY; PURIFICATION; T-CELLS; TOXICITY; VACCINES; YEAST;

ANIMAL MODEL; ANTI-CD3 IMMUNOTOXIN; CELL-BE; CELL/B.E; CELL/BE; DIPHTHERIA TOXIN; EUKARYOTIC PROTEIN SYNTHESIS; IMMUNOTOXINS; IN-VIVO; LYMPH NODE;

MONOCLONAL ANTIBODIES;

A DMDIPTHERIATOXIN390BISCFV IMMUNOTOXIN; ALCOHOL OXIDASE; IMMUNOTOXIN; UNCLASSIFIED DRUG;

ANION EXCHANGE; ARTICLE; CD3+ T LYMPHOCYTE; DRUG PURIFICATION; FUNGAL STRAIN; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; PICHIA PASTORIS; T CELL DEPLETION;

ANIMALIA; EUKARYOTA; PICHIA PASTORIS; SUIDAE;

EID: 80054820606     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/bc200230h     Document Type: Article

References (20)

1. Huang, C. A., Yamada, K., Murphy, M. C., Shimizu, A., Colvin, R. B., Neville, D. M., Jr., and Sachs, D. H. (1999) In vivo T cell depletion in miniature swine using the swine CD3 immunotoxin, pCD3-CRM9 Transplantation 68, 855-860 (Pubitemid 29468205)
2. Fuchimoto, Y., Huang, C. A., Yamada, K., Shimizu, A., Kitamura, H., Colvin, R. B., Ferrara, V., Murphy, M. C., Sykes, M., White-Scharf, M., Neville, D. M., Jr., and Sachs, D. H. (2000) Mixed chimerism and tolerance without whole body irradiation in a large animal model J. Clin. Invest. 105, 1779-1789 (Pubitemid 30421759)
3. Huang, C. A., Fuchimoto, Y., Scheier-Dolberg, R., Murphy, M. C., Neville, D. M., Jr., and Sachs, D. H. (2000) Stable mixed chimerism and tolerance using a nonmyeloablative preparative regimen in a large-animal model J. Clin. Invest. 105, 173-181 (Pubitemid 30054356)
4. Cina, R. A., Wikiel, K. J., Lee, P. W., Cameron, A. M., Hettiarachy, S., Rowland, H., Goodrich, J., Colby, C., Spitzer, T. R., Neville, D. M., Jr., and Huang, C. A. (2006) Stable multilineage chimerism without graft versus host disease following nonmyeloablative haploidentical hematopoietic cell transplantation Transplantation 81, 1677-1685 (Pubitemid 43948301)
5. Neville, D. M., Jr., Scharff, J., Hu, H. Z., Rigaut, K., Shiloach, J., Slingerland, W., and Jonker, M. (1996) A new reagent for the induction of T-cell depletion, anti-CD3-CRM9 J. Immunother. Emphasis. Tumor Immunol. 19, 85-92 (Pubitemid 26139066)
6. Gargollo, P., Yamada, K., Esnaola, N., Fuchimoto, Y., Newell, K. L., Sachs, D. H., and Huang, C. A. (2001) Neuropathy in miniature swine following administration of the mutant diphtheria toxin based immunotoxin, pCD3-CRM9 Transplantation 72, 818-822 (Pubitemid 32879851)
7. Kim, G. B., Wang, Z., Liu, Y. Y., Stavrou, S., Mathias, A., Goodwin, K. J., Thomas, J. M., and Neville, D. M., Jr. (2007) A fold-back single-chain diabody format enhances the bioactivity of an anti-monkey CD3 recombinant diphtheria toxin-based immunotoxin Protein Eng. Des. Sel. 20, 425-432 (Pubitemid 47574739)
8. Woo, J. H., Liu, Y. Y., Mathias, A., Stavrou, S., Wang, Z., Thompson, J., and Neville, D. M., Jr. (2002) Gene optimization is necessary to express a bivalent anti-human anti-T cell immunotoxin in Pichia pastoris Protein Expr. Purif. 25, 270-282 (Pubitemid 35307510)
9. Madshus, I. H., Olsnes, S., and Stenmark, H. (1992) Membrane translocation of diphtheria toxin carrying passenger protein domains Infect. Immun. 60, 3296-3302
10. Hexham, J. M., King, V., Dudas, D., Graff, P., Mahnke, M., Wang, Y. K., Goetschy, J. F, Plattner, D., Zurini, M., Bitsch, F., Lake, P., and Digan, M. E. (2001) Optimization of the anti-(human CD3) immunotoxin DT389-scFv(UCHT1) N-terminal sequence to yield a homogeneous protein Biotechnol. Appl. Biochem. 34, 183-187 (Pubitemid 33152516)
11. Thompson, J., Stavrou, S., Weetall, M., Hexham, J. M., Digan, M. E., Wang, Z., Woo, J. H., Yu, Y., Mathias, A., Liu, Y. Y., Ma, S., Gordienko, I., Lake, P., and Neville, D. M., Jr. (2001) Improved binding of a bivalent single-chain immunotoxin results in increased efficacy for in vivo T-cell depletion Protein Eng. 14, 1035-1041 (Pubitemid 34137244)
12. Frankel, A. E., Zuckero, S. L., Mankin, A. A., Grable, M., Mitchell, K., Lee, Y. J., Neville, D. M., Jr., and Woo, J. H. (2009) Anti-CD3 recombinant diphtheria immunotoxin therapy of cutaneous T cell lymphoma Curr. Drug Targets 10, 104-109
13. Huang, C. A., Lorf, T., Arn, J. S., Koo, G. C., Blake, T., and Sachs, D. H. (1999) Characterization of a monoclonal anti-porcine CD3 antibody Xenotransplant 5, 201-212 (Pubitemid 29439066)
14. Yuan, X., Gubbins, M. J., and Berry, J. D. (2004) A simple and rapid protocol for the sequence determination of functional kappa light chain cDNAs from aberrant-chain-positive murine hybridomas J. Immunol. Methods 294, 199-207 (Pubitemid 39626604)
15. Liu, Y. Y., Gordienko, I., Mathias, A., Ma, A., Thompson, J., Woo, J. H., and Neville, D. M., Jr. (2000) Expression of an anti-CD3 single-chain immunotoxin with a truncated diphtheria toxin in a mutant CHO cell line Protein Expr. Purif. 19, 304-311 (Pubitemid 30446311)
16. Sreekrishna, K. (1993). Strategies for optimizing protein expression and secretion in the methylotrophic yeast Pichia pastoris. In Industrial Microorganism: Basic and Applied Molecular Genetics (Baltz, R. H., Hegeman, G. D., and, and Skatrud, P. L., Eds.) pp 119-126, American Society of Microbiology, Washington, DC.
17. Liu, Y. Y., Woo, J. H., and Neville, D. M., Jr. (2003) Targeted introduction of a diphtheria toxin resistant mutation into the chromosomal EF-2 locus of Pichia pastoris and expression of immunotoxin in the EF-2 mutants Protein Expr. Purif. 30, 262-274 (Pubitemid 36990340)
18. Kabat, E. A., Wu, T. T., Reid-Miller, M., and Gottesman, K. S. (1987) Sequences of proteins of immunological interest, U.S. Department of Health and Human Services, U.S. Governmental Printing Office, Washington, DC.
19. Woo, J. H. and Neville, D. M., Jr. (2003) Separation of bivalent anti-T cell immunotoxin from Pichia pastoris glycoproteins by borate anion exchange BioTechniques 35, 392-398 (Pubitemid 36994342)
20. Wang, Z., Kim, G. B., Woo, J. H., Liu, Y. Y., Mathias, A., Stavrou, S., and Neville, D. M., Jr. (2007) Improvement of a recombinant anti-monkey anti-CD3 diphtheria toxin based immunotoxin by yeast display affinity maturation of the scFv Bioconjugate Chem. 18, 947-955 (Pubitemid 47010818)