Expression of an anti-CD3 single-chain immunotoxin with a truncated diphtheria toxin in a mutant CHO cell line

Protein Expression and Purification

Volumn 19, Issue 2, 2000, Pages 304-311

  Liu, Yuan Yi ^a   Gordienko, Irina ^a   Mathias, Askale ^a   Ma, Shenglin ^b   Thompson, Jerry ^c   Woo, Jung Hee ^a   Neville Jr , David M ^a  

^a NATIONAL INSTITUTE OF MENTAL HEALTH   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c 104 Fenske Laboratory   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTI CD3 SINGLE CHAIN IMMUNOTOXIN; BACTERIAL TOXIN; CELL SECRETION; CHO CELL; CYTOTOXICITY; DISULFIDE BOND; GLYCOSYLATION; IMMUNOTOXIN; MUTATION; N GLYCOSIDASE; PROTEIN FOLDING; T LYMPHOCYTE;

BACTERIA (MICROORGANISMS); CRICETINAE; ESCHERICHIA COLI; GENETTA;

EID: 0000147018     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1255     Document Type: Article

References (38)

1. Choe S., Bennett M. J., Fujii G., Cumri P. M. G., Kantardjieff F. A., Collier R. J., Eisenberg D. The crystal structure of diphtheria toxin. Nature. 357:1992;216-222.
2. Naglich J. G., Metherall J. E., Russell D. W., Eidels L. Expression cloning of a diphtheria toxin receptor: Identity with a heparin-binding EGF-like growth factor precursor. Cell. 69:1992;1051-1061.
3. Gordon V. M., Klimpel K. R., Arora N., Henderson M. A., Leppla S. H. Proteolytic activation of bacterial toxins by eukaryotic cells is performed by furin and by additional cellular proteases. Infect. Immun. 63:1995;82-87.
4. London E. Diphtheria toxin: Membrane interaction and membrane translocation. Biochim. Biophys. Acta. 1113:1992;25-51.
5. Van Ness B. G., Howard J. B., Bodley J. W. ADP-ribosylation of elongation factor 2 by diphtheria toxin. J. Biol. Chem. 255:1980;10717-10720.
6. Bodley J. W., Uphan R., Crow F. W., Tomer K. B., Gross M. L. Ribosyl-diphthamide: Confirmation of structure by fast atom bombardment mass spectrometry. Arch. Biochem. Biophys. 230:1984;590-593.
7. Brown B. A., Bodley J. W. Primary structure at the site in beef and wheat elongation factor 2 of ADP-ribosylation by diphtheria toxin. FEBS Lett. 103:1979;253-255.
8. Pappenheimer A. M. Jr., Dunlop P. C., Adolph K. W., Bodley J. W. Occurrence of diphthamide in archaebacteria. J. Bacteriol. 153:1983;1342-1347.
9. Murphy J. R., van der Spek J. C. Targeting diphtheria toxin to growth factor receptors. Semin. Cancer Biol. 6:1995;259-267.
10. Youle R. J., Greenfield L., Johnson V. G. Genetic engineering of immunotoxins. Frankel A. E. Immunotoxins. 1988;113-122 Kluwer, Boston.
11. Nicholls P. J., Youle R. J. The structure of diphtheria toxin as a guide to rational design. Frankel A. E. Genetically Engineered Toxins. 1992;339-363 Dekker, New York.
12. Neville D. M. Jr., Scharff J., Srinivasachar K. In vivo T-cell ablation by a holo-immunotoxin directed at human CD3. Proc. Natl. Acad. Sci. USA. 89:1992;2585-2589.
13. Foss F. M., Saleh M. N., Krueger J. G., Nichols J. C., Murphy J. R. Diphtheria toxin fusion proteins. Curr. Top. Microbiol. Immunol. 234:1998;63-81.
14. Ma S., Hu H., Thompson J., Stavrou S., Scharff J., Neville D. M. Jr. Genetic construction and characterization of an anti-monkey CD3 single-chain immunotoxin with a truncated diphtheria toxin. Bioconjug. Chem. 8:1997;695-701.
15. Thompson J., Hu H., Scharff J., Neville D. M. Jr. An anti-CD3 single-chain immunotoxin with a truncated diphtheria toxin avoids inhibition by pre-existing antibodies in human blood. J. Biol. Chem. 270:1995;28037-28041.
16. Kreitman R. J., Pastan I. Recombinant toxins. Adv. Pharmacol. 28:1994;193-219.
17. 390-anti-CD3sFv, a single-chain Fv fusion immunotoxin specifically targeting the CD3 moiety of the T-cell receptor. Blood. 88:1996;2342-2353.
18. Buchner J., Pastan I., Brinkmann U. A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies. Anal. Biochem. 205:1992;263-270.
19. Frankel A. E., Ramage J., Latimer A., Feely T., Delatte S., Hall P., Tagge E., Kreitman R., Willingham M. High-level expression and purification of the recombinant diphtheria fusion toxin DTGM for phase 1 clinical trials. Protein Express. Purif. 16:1999;190-210.
20. Bishai W. R., Miyanohara A., Murphy J. R. Cloning and expression in Escherichia coli of three fragments of diphtheria toxin truncated within fragment B. J. Bacteriol. 169:1987;1554-1563.
21. Bishai W. R., Rappuoli R., Murphy J. R. High-level expression of a proteolytically sensitive diphtheria toxin fragment in Escherichia coli. J. Bacteriol. 169:1987;5140-5151.
22. O'Keefe D. O., Collier R. J. Cloned diphtheria toxin within the periplasm of Escherichia coli causes lethal membrane damage at low pH. Proc. Natl. Acad. Sci. USA. 86:1989;343-346.
23. Chen J. Y C., Bodley J. W., Livingston D. M. Diphtheria toxin-resistant mutants of Saccharomyces cerevisiae. Mol. Cell. Biol. 5:1985;3357-3360.
24. Perentesis J. P., Genbauffe F. S., Veldman S. A., Galeotti C. L., Livingston D. M., Bodley J. W., Murphy J. R. Expression of diphtheria toxin fragment A and hormone-toxin fusion proteins in toxin-resistant yeast mutants. Proc. Natl. Acad. Sci. USA. 85:1988;8386-8390.
25. Moehring J. M., Moehring T. J. Characterization of the diphtheria toxin-resistance system in chinese hamster ovary cells. Somat. Cell Genet. 5:1979;453-468.
26. Foley B. T., Moehring J. M., Moehring T. J. A mutation in codon 717 of the Cho-K1 elongation factor 2 gene prevents the first step in the biosynthesis of diphthamide. Somat. Cell Mol. Genet. 18:1992;227-231.
27. Takebe Y., Seiki M., Fujisawa J., Hoy P., Yokota K., Arai K., Yoshida M., Arai N. SRα promoter: An efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell Biol. 8:1988;466-472.
28. Von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur. J. Biochem. 133:1983;17-21.
29. Elbein A. D. Inhibitors of glycoprotein synthesis. Methods Enzymol. 98:1983;135-154.
30. Chen S. Y., Yang A. G., Chen J. D., Kute T., King C. R., Collier J., Cong Y., Yao C., Huang X. F. Potent antitumour activity of a new class of tumour-specific killer cells. Nature. 385:1997;78-80.
31. Thomas J. M., Contreras J. L., Jiang X. L., Eckhoff D. E., Wang P. X., Hubbard W. J., Lobashevsky A. L., Wang W., Asiedu C., Stavrou S., Cook W. J., Robbin M. L., Thomas F. T., Neville D. M. Jr. Peritransplant tolerance induction in macaques: Early events reflecting the unique synergy between immunotoxin and deoxyspergualin. Transplantation. 68:1999;1660-1673.
32. Thomas F. T., Ricordi C., Contreras J. L., Hubbard W. J., Jiang X. L., Eckhoff D. E., Cartner S., Bibao G., Neville D. M. Jr., Thomas J. M. Reversal of naturally occuring diabetes in primates by unmodified islet xenografts without chronic immunosuppression. Transplantation. 67:1999;846-854.
33. Malenbaum S. E., Collier R. J., London E. Membrane topography of the T domain of diphtheria toxin probed with single tryptophan mutants. Biochemistry. 37:1998;17915-17922.
34. Hogue C. W. Cn3D: A new generation of three-dimensional molecular structure viewer. Trends Biochem. Sci. 22:1997;314-316.
35. Bennett M. J., Choe S., Eisenberg D. Refined structure of dimeric diphtheria toxin at 2.0 Å resolution. Protein Sci. 3:1994;1444-1463.
36. Berman H. M., Westbrook J., Feng Z., Gilliland G., Bhat T. N., Weissig H., Shindyalov I. N., Bourne P. E. The protein data bank. Nucleic Acids Res. 28:2000;235-242.
37. Stewart E. J., Åslund F., Beckwith J. Disulfide bond formation in the Escherichia coli cytoplasm: An in vivo role reversal for the thioredoxins. EMBO J. 17:1998;5543-5550.
38. Goochee C. F., Gramer M. J., Andersen D. C., Bahr J. B., Rasmussen J. R. The oligosaccharides of glycoproteins: Bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties. Bio/Technology. 9:1991;1347-1355.