메뉴 건너뛰기




Volumn 789, Issue , 2011, Pages 111-126

Direct In Situ RT-PCR

Author keywords

Alkaline phosphatase; CGRP; Digoxigenin; Histology; In situ hybridization; In situ PCR; mRNA; Neuropeptides; Reverse transcription

Indexed keywords

ALKALINE PHOSPHATASE; CALCITONIN GENE RELATED PEPTIDE; DIGOXIGENIN; MESSENGER RNA; NEUROPEPTIDE;

EID: 80054773454     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-310-3_6     Document Type: Chapter
Times cited : (10)

References (32)
  • 1
    • 0026705725 scopus 로고
    • Sensitive mRNA detection using unfixed tissue: Combined radioactive and non-radioactive in situ hybridization histochemistry
    • Dagerlind, A., Friberg, K., Bean, A. J., and Hökfelt, T. (1992) Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry. Histochemistry 98, 39–49.
    • (1992) Histochemistry , vol.98 , pp. 39-49
    • Dagerlind, A.1    Friberg, K.2    Bean, A.J.3    Hökfelt, T.4
  • 2
    • 0028692109 scopus 로고
    • Hybridization histochemistry using radiola-beled oligodeoxyribonucleotide probes
    • Penschow, J. D. and Coghlan, J. P. (1994) Hybridization histochemistry using radiola-beled oligodeoxyribonucleotide probes. Methods Mol.Biol. 33, 277–292.
    • (1994) Methods Mol.Biol. , vol.33 , pp. 277-292
    • Penschow, J.D.1    Coghlan, J.P.2
  • 3
    • 0028694355 scopus 로고
    • Subcellular location of mRNA by electron microscope hybridization histochemistry
    • Penschow, J. D. and Coghlan, J. P. (1994) Subcellular location of mRNA by electron microscope hybridization histochemistry. Methods Mol.Biol. 33, 345–358.
    • (1994) Methods Mol.Biol. , vol.33 , pp. 345-358
    • Penschow, J.D.1    Coghlan, J.P.2
  • 4
    • 0033990116 scopus 로고    scopus 로고
    • In situ localization of PCR-amplified DNA and cDNA
    • Nuovo, G. J. (2000) In situ localization of PCR-amplified DNA and cDNA. Methods Mol. Biol. 123, 217–238.
    • (2000) Methods Mol. Biol. , vol.123 , pp. 217-238
    • Nuovo, G.J.1
  • 5
    • 0034772411 scopus 로고    scopus 로고
    • Co-labeling using in situ PCR: A review
    • Nuovo, G. J. (2001) Co-labeling using in situ PCR: a review. J.Histochem.Cytochem. 49, 1329–1339.
    • (2001) J.Histochem.Cytochem. , vol.49 , pp. 1329-1339
    • Nuovo, G.J.1
  • 6
    • 0025326473 scopus 로고
    • Amplification and detection of lentiviral DNA inside cells
    • Haase, A. T., Retzel, E. F., and Staskus, K. A. (1990) Amplification and detection of lentiviral DNA inside cells. Proc.Natl.Acad.Sci.U.S.A 87, 4971–4975.
    • (1990) Proc.Natl.Acad.Sci.U.S.A , vol.87 , pp. 4971-4975
    • Haase, A.T.1    Retzel, E.F.2    Staskus, K.A.3
  • 7
    • 0027301880 scopus 로고
    • In-situ polymerase chain reaction. An overview of methods, applications and limitations of a new molecular technique
    • Komminoth, P. and Long, A. A. (1993) In-situ polymerase chain reaction. An overview of methods, applications and limitations of a new molecular technique. Virchows Arch.B Cell Pathol.Incl.Mol.Pathol. 64, 67–73.
    • (1993) Virchows Arch.B Cell Pathol.Incl.Mol.Pathol. , vol.64 , pp. 67-73
    • Komminoth, P.1    Long, A.A.2
  • 10
    • 33746871480 scopus 로고    scopus 로고
    • In situ PCR protocols
    • Bagasra, O. and Harris, T. (2006) In situ PCR protocols. Methods Mol.Biol. 334, 61–78.
    • (2006) Methods Mol.Biol , vol.334 , pp. 61-78
    • Bagasra, O.1    Harris, T.2
  • 11
    • 33746890888 scopus 로고    scopus 로고
    • Latest developments in in situ PCR
    • Bagasra, O. and Harris, T. (2006) Latest developments in in situ PCR. Methods Mol.Biol. 334:221–40., 221–240.
    • (2006) Methods Mol.Biol. , vol.334 , pp. 221-240
    • Bagasra, O.1    Harris, T.2
  • 12
    • 39149134332 scopus 로고    scopus 로고
    • Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences
    • Bagasra, O. (2007) Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences. Nat.Protoc. 2, 2782–2795.
    • (2007) Nat.Protoc. , vol.2 , pp. 2782-2795
    • Bagasra, O.1
  • 13
    • 85139029736 scopus 로고    scopus 로고
    • In situ polymerase chain reaction and hybridization to detect low-abundance nucleic acid targets
    • Bagasra, O. (2008) In situ polymerase chain reaction and hybridization to detect low-abundance nucleic acid targets. Curr.Protoc. Mol.Biol. Chapter 14:Unit 14.8.
    • (2008) Curr.Protoc. Mol.Biol. Chapter , vol.14 , pp. Unit 14.8
    • Bagasra, O.1
  • 14
    • 42449153846 scopus 로고    scopus 로고
    • The gastrointestinal hormone ghrelin modulates inhibitory neurotransmission in deep laminae of mouse spinal cord dorsal horn
    • Vergnano, A. M., Ferrini, F., Salio, C., Lossi, L., Baratta, M., and Merighi, A. (2008) The gastrointestinal hormone ghrelin modulates inhibitory neurotransmission in deep laminae of mouse spinal cord dorsal horn. Endocrinology. 149, 2306–2312.
    • (2008) Endocrinology , vol.149 , pp. 2306-2312
    • Vergnano, A.M.1    Ferrini, F.2    Salio, C.3    Lossi, L.4    Baratta, M.5    Merighi, A.6
  • 15
    • 77953025205 scopus 로고    scopus 로고
    • Modulation of inhibitory neurotransmission by the vanilloid receptor type 1 (TRPV1) in organotypically cultured mouse substantia gelatinosa neurons
    • Ferrini, F., Salio, C., Lossi, L., Gambino, G., and Merighi, A. (2010) Modulation of inhibitory neurotransmission by the vanilloid receptor type 1 (TRPV1) in organotypically cultured mouse substantia gelatinosa neurons. Pain. 150, 128–140.
    • (2010) Pain , vol.150 , pp. 128-140
    • Ferrini, F.1    Salio, C.2    Lossi, L.3    Gambino, G.4    Merighi, A.5
  • 16
    • 70349934573 scopus 로고    scopus 로고
    • The role of peptides in central sensitization
    • Seybold, V. S. (2009) The role of peptides in central sensitization. Handb.Exp.Pharmacol. 451–491.
    • (2009) Handb.Exp.Pharmacol. , pp. 451-491
    • Seybold, V.S.1
  • 18
    • 33747412217 scopus 로고    scopus 로고
    • Neuron-glia interactions at the neuromuscular synapse
    • Todd, K. J. and Robitaille, R. (2006) Neuron-glia interactions at the neuromuscular synapse. Novartis.Found.Symp. 276, 222–229.
    • (2006) Novartis.Found.Symp. , vol.276 , pp. 222-229
    • Todd, K.J.1    Robitaille, R.2
  • 19
    • 69249202445 scopus 로고    scopus 로고
    • alphaCGRP and betaCGRP transcript amount in mouse tissues of various developmental stages and their tissue expression sites
    • Rezaeian, A. H., Isokane, T., Nishibori, M., Chiba, M., Hiraiwa, N., Yoshizawa, M., and Yasue, H. (2009) alphaCGRP and betaCGRP transcript amount in mouse tissues of various developmental stages and their tissue expression sites. Brain Dev. 31, 682–693.
    • (2009) Brain Dev , vol.31 , pp. 682-693
    • Rezaeian, A.H.1    Isokane, T.2    Nishibori, M.3    Chiba, M.4    Hiraiwa, N.5    Yoshizawa, M.6    Yasue, H.7
  • 20
  • 22
    • 58049103314 scopus 로고    scopus 로고
    • Theory and practice of combining coagulant fixation and microwave histoprocessing
    • Boon, M. E. and Kok, L. P. (2008) Theory and practice of combining coagulant fixation and microwave histoprocessing. Biotech.Histochem. 83, 261–277.
    • (2008) Biotech.Histochem. , vol.83 , pp. 261-277
    • Boon, M.E.1    Kok, L.P.2
  • 23
    • 41749093017 scopus 로고    scopus 로고
    • Effects of processing delay, formalin fixation, and immunohistochemistry on RNA Recovery From Formalin-fixed Paraffin-embedded Tissue Sections
    • van, M. F., de, W. M., Morsink, F., Musler, A., Weegenaar, J., and van Noesel, C. J. (2008) Effects of processing delay, formalin fixation, and immunohistochemistry on RNA Recovery From Formalin-fixed Paraffin-embedded Tissue Sections. Diagn.Mol.Pathol. 17, 51–58.
    • (2008) Diagn.Mol.Pathol. , vol.17 , pp. 51-58
    • Van, M.F.1    De, W.M.2    Morsink, F.3    Musler, A.4    Weegenaar, J.5    van Noesel, C.J.6
  • 26
    • 0142106515 scopus 로고    scopus 로고
    • A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples
    • Vincek, V., Nassiri, M., Nadji, M., and Morales, A. R. (2003) A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab Invest. 83, 1427–1435.
    • (2003) Lab Invest , vol.83 , pp. 1427-1435
    • Vincek, V.1    Nassiri, M.2    Nadji, M.3    Morales, A.R.4
  • 27
    • 0028609272 scopus 로고
    • Analysis of nonspecific DNA synthesis during in situ PCR and solution-phase PCR
    • Nuovo, G. J., MacConnell, P., and Gallery, F. (1994) Analysis of nonspecific DNA synthesis during in situ PCR and solution-phase PCR. PCR Methods Appl. 4, 89–96.
    • (1994) PCR Methods Appl , vol.4 , pp. 89-96
    • Nuovo, G.J.1    Macconnell, P.2    Gallery, F.3
  • 28
    • 0034880639 scopus 로고    scopus 로고
    • Direct in situ reverse transcriptase-polymerase chain reaction
    • Kher, R. and Bacallao, R. (2001) Direct in situ reverse transcriptase-polymerase chain reaction. Am.J.Physiol Cell Physiol. 281, C726–C732.
    • (2001) Am.J.Physiol Cell Physiol. , vol.281 , pp. C726-C732
    • Kher, R.1    Bacallao, R.2
  • 29
    • 7144230803 scopus 로고    scopus 로고
    • Enzymatic amplification of DNA by PCR: Standard procedures and optimization. Curr.Protoc. Immunol
    • Kramer, M. F. and Coen, D. M. (2001) Enzymatic amplification of DNA by PCR: standard procedures and optimization. Curr.Protoc. Immunol. Chapter 10:Unit 10.20.
    • (2001) Chapter 10:Unit , vol.10 , pp. 20
    • Kramer, M.F.1    Coen, D.M.2
  • 30
    • 0242709862 scopus 로고    scopus 로고
    • GAP-43 mRNA detection by in situ hybridization, direct and indirect in situ RT-PCR in hippocampal and cerebellar tissue sections of adult rat brain
    • Casoli, T., Stefano, G. D., Fattoretti, P., Solazzi, M., Delfino, A., Biagini, G., and Bertoni-Freddari, C. (2003) GAP-43 mRNA detection by in situ hybridization, direct and indirect in situ RT-PCR in hippocampal and cerebellar tissue sections of adult rat brain. Micron. 34, 415–422.
    • (2003) Micron , vol.34 , pp. 415-422
    • Casoli, T.1    Stefano, G.D.2    Fattoretti, P.3    Solazzi, M.4    Delfino, A.5    Biagini, G.6    Bertoni-Freddari, C.7
  • 31
    • 33745908583 scopus 로고    scopus 로고
    • Treatment of tissue sections for in situ hybridization
    • Tesch, G. H., Lan, H. Y., and Nikolic-Paterson, D. J. (2006) Treatment of tissue sections for in situ hybridization. Methods Mol.Biol. 326, 1–7.
    • (2006) Methods Mol.Biol. , vol.326 , pp. 1-7
    • Tesch, G.H.1    Lan, H.Y.2    Nikolic-Paterson, D.J.3
  • 32
    • 0030612160 scopus 로고    scopus 로고
    • Self-seal reagent: Evaporation control for molecular histology procedures without chambers, clips or fingernail polish
    • Sullivan, D. E., Bobroski, L. E., Bagasra, O., and Finney, M. (1997) Self-seal reagent: evaporation control for molecular histology procedures without chambers, clips or fingernail polish. Biotechniques. 23, 320–325.
    • (1997) Biotechniques , vol.23 , pp. 320-325
    • Sullivan, D.E.1    Bobroski, L.E.2    Bagasra, O.3    Finney, M.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.