메뉴 건너뛰기




Volumn 2, Issue 11, 2007, Pages 2782-2795

Protocols for the in situ pcr-amplification and detection of mrna and dna sequences

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MESSENGER RNA; PRIMER DNA; PROTEINASE K;

EID: 39149134332     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.395     Document Type: Article
Times cited : (65)

References (36)
  • 2
    • 0028070306 scopus 로고
    • Potential of in situ polymerase chain reaction in diagnostic cytology
    • Pestaner, J.P., Bibbo, M., Bobroski, L., Seshamma, T. & Bagasra, O. Potential of in situ polymerase chain reaction in diagnostic cytology. Acta Cytol. 38, 676-680 (1994).
    • (1994) Acta Cytol , vol.38 , pp. 676-680
    • Pestaner, J.P.1    Bibbo, M.2    Bobroski, L.3    Seshamma, T.4    Bagasra, O.5
  • 3
    • 0027201999 scopus 로고
    • Detection of HIV-I DNA and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry
    • Patterson, B. etal. Detection of HIV-I DNA and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry. Science 260, 976-979 (1993).
    • (1993) Science , vol.260 , pp. 976-979
    • Patterson, B.E.1
  • 4
    • 16044372349 scopus 로고    scopus 로고
    • A herpes simplex virus mutant with a deletion immediately upstream of the LAT locus establishes latency and reactivates from latently infected mice with normal kinetics
    • Maggioncalda, J., Mehta, A., Bagasra, O., Fraser, N.W. & Block, T.M. A herpes simplex virus mutant with a deletion immediately upstream of the LAT locus establishes latency and reactivates from latently infected mice with normal kinetics. J. Neurovirol. 2, 268-278 (1996).
    • (1996) J. Neurovirol , vol.2 , pp. 268-278
    • Maggioncalda, J.1    Mehta, A.2    Bagasra, O.3    Fraser, N.W.4    Block, T.M.5
  • 5
    • 0030612160 scopus 로고    scopus 로고
    • Self-seal reagent: Evporation control for molecular histology procedure without chambers, clips or fingernail polish
    • Sullivan, D.E., Bobroski, L.E., Bagasra, O. & Finney, M. Self-seal reagent: evporation control for molecular histology procedure without chambers, clips or fingernail polish. BioTechniques 23, 320-225 (1997).
    • (1997) Biotechniques , vol.23 , pp. 320-225
    • Sullivan, D.E.1    Bobroski, L.E.2    Bagasra, O.3    Finney, M.4
  • 6
    • 0027483712 scopus 로고
    • HIV-1 latency due to the site of proviral integration
    • Winslow, B.J., Pomerantz, R.J., Bagasra, O. & Trono, D. HIV-1 latency due to the site of proviral integration. Virology 196, 849-854 (1993).
    • (1993) Virology , vol.196 , pp. 849-854
    • Winslow, B.J.1    Pomerantz, R.J.2    Bagasra, O.3    Trono, D.4
  • 7
    • 0032415082 scopus 로고    scopus 로고
    • Localization of human herpes virus type 8 (HHV-8) in the Kaposi's sarcoma tissues and the semen specimens of HIV-1 infected and uninfected individuals by utilizing in situ polymerase chain reaction
    • Bobroski, L. et al. Localization of human herpes virus type 8 (HHV-8) in the Kaposi's sarcoma tissues and the semen specimens of HIV-1 infected and uninfected individuals by utilizing in situ polymerase chain reaction. J. Reprod. Immunol. 41, 149-160 (1999).
    • (1999) J. Reprod. Immunol , vol.41 , pp. 149-160
    • Bobroski, L.1
  • 8
    • 0027389013 scopus 로고
    • Analysis of human immunodeficiency virus-infected tissues by amplification and in situ hybridization reveals latent and permissive infections at single-cell resolution
    • Embretson, J. et al. Analysis of human immunodeficiency virus-infected tissues by amplification and in situ hybridization reveals latent and permissive infections at single-cell resolution. Proc. Natl. Acad. Sci. USA 90, 357-361 (1993).
    • (1993) Proc. Natl. Acad. Sci. USA , vol.90 , pp. 357-361
    • Embretson, J.1
  • 9
    • 0027516822 scopus 로고
    • Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period ofAIDS
    • Embretson, J. et al. Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period ofAIDS. Nature 362, 359-362 (1993).
    • (1993) Nature , vol.362 , pp. 359-362
    • Embretson, J.1
  • 10
    • 0036001213 scopus 로고    scopus 로고
    • The presence ofIL-2 and IL-10 cytokines in the skin lesions of Blau syndrome. Appl. Immunohistochem. Mol
    • Ewida, A.S., Raphael, S. & Bagasra, O. The presence ofIL-2 and IL-10 cytokines in the skin lesions of Blau syndrome. Appl. Immunohistochem. Mol. Morphol. 10, 171-177 (2002).
    • (2002) Morphol , vol.10 , pp. 171-177
    • Ewida, A.S.1    Raphael, S.2    Bagasra, O.3
  • 11
    • 19244362046 scopus 로고    scopus 로고
    • Human herpes virus 8 (HHV-8) DNA sequences in cell- free plasma and mononuclear cells of Kaposi's sarcoma patients
    • Harrington, W.J. Jr. et al. Human herpes virus 8 (HHV-8) DNA sequences in cell- free plasma and mononuclear cells of Kaposi's sarcoma patients. J. Infect. Dis. 174, 1101-1105 (1996).
    • (1996) J. Infect. Dis , vol.174 , pp. 1101-1105
    • Harrington, W.J.1
  • 13
    • 0026510316 scopus 로고
    • Detection of human immunodeficiency virus type 1 provirus in mononuclear cells by in situ polymerase chain reaction
    • Bagasra, O., Hauptman, S.P., Lischner, H.W., Sachs, M. & Pomerantz, R.J. Detection of human immunodeficiency virus type 1 provirus in mononuclear cells by in situ polymerase chain reaction. N. Engl. J. Med. 326, 1385-1391 (1992).
    • (1992) N. Engl. J. Med , vol.326 , pp. 1385-1391
    • Bagasra, O.1    Hauptman, S.P.2    Lischner, H.W.3    Sachs, M.4    Pomerantz, R.J.5
  • 14
    • 0027386692 scopus 로고
    • Polymerase chain reaction in situ: Intracellularamplification and detection of HIV-1 proviral DNA and otherspecific genes
    • Bagasra, O., Seshamma, T. & Pomerantz, R.J. Polymerase chain reaction in situ: intracellularamplification and detection of HIV-1 proviral DNA and otherspecific genes. J. Immunol. Methods 158, 131-145 (1993).
    • (1993) J. Immunol. Methods , vol.158 , pp. 131-145
    • Bagasra, O.1    Seshamma, T.2    Pomerantz, R.J.3
  • 15
    • 0027437293 scopus 로고
    • High percentages ofCD4- positive lymphocytes harbor the HIV-1 provirus in the blood of certain infected individuals
    • Bagasra, O., Seshamma, T., Oakes, J. & Pomerantz, R.J. High percentages ofCD4- positive lymphocytes harbor the HIV-1 provirus in the blood of certain infected individuals. AIDS 7, 1419-1425 (1993).
    • (1993) AIDS , vol.7 , pp. 1419-1425
    • Bagasra, O.1    Seshamma, T.2    Oakes, J.3    Pomerantz, R.J.4
  • 16
    • 0028046322 scopus 로고
    • Human immunodeficiency virus type 1 infection ofsperm in vivo
    • Bagasra, O. etal. Human immunodeficiency virus type 1 infection ofsperm in vivo. AIDS 8, 1669-1674 (1994).
    • (1994) AIDS , vol.8 , pp. 1669-1674
    • Bagasra, O.E.1
  • 17
    • 0029592005 scopus 로고
    • Activation ofthe inducible form ofnitric oxide synthetase in the brains of patients with multiple sclerosisProc. Natl. Acad. Sci. USA
    • Bagasra, O. etal. Activation ofthe inducible form ofnitric oxide synthetase in the brains of patients with multiple sclerosis. Proc. Natl. Acad. Sci. USA 92, 12041¬12045 (1995).
    • (1995) , vol.92 , pp. 12041-12045
    • Bagasra, O.1
  • 18
    • 85044683047 scopus 로고    scopus 로고
    • Human herpesvirus 8 DNA sequences in CD8+ T-cells (Let)
    • Sirianni, M.C. et al. Human herpesvirus 8 DNA sequences in CD8+ T-cells (Let). J. Infect. Dis. 176, 541 (1997).
    • (1997) J. Infect. Dis , vol.176 , pp. 541
    • Sirianni, M.C.1
  • 19
    • 0342528101 scopus 로고
    • In situ polymerase chain reaction and hybridization to detect low abundance nucleic acid targets
    • (eds. Ausubel etal., John Wiley & Sons, New York
    • Bagasra, O. et al. In situ polymerase chain reaction and hybridization to detect low abundance nucleic acid targets. In CurrentProtocols in MolecularBiology (eds. Ausubel etal.) 1-49 (John Wiley & Sons, New York, 1995).
    • (1995) Currentprotocols in Molecularbiology , pp. 1-49
    • Bagasra, O.1
  • 20
    • 0030011404 scopus 로고    scopus 로고
    • Cellular reservoirs of HIV-1 in the central nervous system of infected-individuals: Identification by the combination of in situ polymerase chain reaction and immunohistochemistry
    • Bagasra, O. et al. Cellular reservoirs of HIV-1 in the central nervous system of infected-individuals: identification by the combination of in situ polymerase chain reaction and immunohistochemistry. AIDS 10, 573-585 (1996).
    • (1996) AIDS , vol.10 , pp. 573-585
    • Bagasra, O.1
  • 21
    • 0033862738 scopus 로고    scopus 로고
    • Protection against retroviruses are owing to a different form of immunity. An RNA-based molecular immunity hypothesis. Appl. Immunohistochem. Mol
    • Bagasra, O. & Amjad, M. Protection against retroviruses are owing to a different form of immunity. An RNA-based molecular immunity hypothesis. Appl. Immunohistochem. Mol. Morphol. 8, 133-146 (2000).
    • (2000) Morphol , vol.8 , pp. 133-146
    • Bagasra, O.1    Amjad, M.2
  • 22
    • 0032763187 scopus 로고    scopus 로고
    • Mechanism ofverticaltransmission ofHIV-1: Role ofintervillous space. Appl. Immunohistochem. Mol
    • Bobroski, L. etal. Mechanism ofverticaltransmission ofHIV-1: role ofintervillous space. Appl. Immunohistochem. Mol. Morphol. 7, 271-279 (1999).
    • (1999) Morphol , vol.7 , pp. 271-279
    • Bobroski, L.E.1
  • 23
    • 0032919967 scopus 로고    scopus 로고
    • Multicenter comparison of PCR assays for detection of human herpesvirus 8 DNA in semen
    • Pellett, P.E. et al. Multicenter comparison of PCR assays for detection of human herpesvirus 8 DNA in semen. J. Clin. Microbiol. 37, 1298-1301 (1999).
    • (1999) J. Clin. Microbiol , vol.37 , pp. 1298-1301
    • Pellett, P.E.1
  • 24
    • 84988046221 scopus 로고
    • Localization of HIV-1 proviral DNA in oral mucosal epithelial cells
    • Qureshi, M.N. et al. Localization of HIV-1 proviral DNA in oral mucosal epithelial cells. J. Infect. Dis. 171, 190-193 (1994).
    • (1994) J. Infect. Dis , vol.171 , pp. 190-193
    • Qureshi, M.N.1
  • 25
    • 0030947050 scopus 로고    scopus 로고
    • Titering replication-defective virus for use in gene transfer
    • Strayer, D.S. et al. Titering replication-defective virus for use in gene transfer. BioTechniques 22, 447-451 (1997).
    • (1997) Biotechniques , vol.22 , pp. 447-451
    • Strayer, D.S.1
  • 26
    • 0029000896 scopus 로고
    • Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice
    • Pereira, R.F. etal. Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice. Proc. Natl. Acad. Sci. USA 92, 4857-4861 (1995).
    • (1995) Proc. Natl. Acad. Sci. USA , vol.92 , pp. 4857-4861
    • Pereira, R.F.1
  • 27
    • 12644261423 scopus 로고    scopus 로고
    • Prevention of experimental allergic encephalomyelitis by targeting nitric oxide and peroxynitrite: Implications for the treatment of multiple sclerosis
    • Hooper, D.C. etal. Prevention of experimental allergic encephalomyelitis by targeting nitric oxide and peroxynitrite: implications for the treatment of multiple sclerosis. Proc. Natl. Acad. Sci. USA 94, 2528-2533 (1997).
    • (1997) Proc. Natl. Acad. Sci. USA , vol.94 , pp. 2528-2533
    • Hooper, D.C.E.1
  • 28
    • 0029126344 scopus 로고
    • Expression of cytokine mRNA in human melanoma tissue
    • Lattime, E.C. etal. Expression of cytokine mRNA in human melanoma tissue. Cancer Immunol. Immunother. 41, 151-156 (1995).
    • (1995) Cancer Immunol. Immunother , vol.41 , pp. 151-156
    • Lattime, E.C.1
  • 29
    • 26844565027 scopus 로고    scopus 로고
    • HZIP1 zinc uptake transporter down regulation and zinc depletion in prostate cancer. Mol
    • Franklin, R.B. et al. hZIP1 zinc uptake transporter down regulation and zinc depletion in prostate cancer. Mol. Cancer 4, 32 (2005).
    • (2005) Cancer , vol.4 , pp. 32
    • Franklin, R.B.1
  • 30
    • 84988044760 scopus 로고    scopus 로고
    • Down-regulation of hZIP1 and hZIP2 zinc transporters in the prostate cancer tissues from African decent as compared to Caucasian men. Appl
    • Rishi, I.R. etal. Down-regulation of hZIP1 and hZIP2 zinc transporters in the prostate cancer tissues from African decent as compared to Caucasian men. Appl. Immunohistochem. Mol. Morphol. 10, 171-177 (2003).
    • (2003) Immunohistochem. Mol. Morphol , vol.10 , pp. 171-177
    • Rishi, I.R.1
  • 32
    • 33646890873 scopus 로고    scopus 로고
    • Localization of human herpes virus type 8 in human semen by in situ PCR
    • Bagasra, O. etal. Localization of human herpes virus type 8 in human semen by in situ PCR. J. Mol. Histol. 36, 401-412 (2006).
    • (2006) J. Mol. Histol , vol.36 , pp. 401-412
    • Bagasra, O.1
  • 33
    • 0031958235 scopus 로고    scopus 로고
    • Development ofa direct in situ PCR method for detection of specific bacteria in natural environments. Appl. Environ
    • Tani, K., Kurokawa, K. & Nasu, M. Development ofa direct in situ PCR method for detection of specific bacteria in natural environments. Appl. Environ. Microbiol. 64, 1536-1540 (1998).
    • (1998) Microbiol , vol.64 , pp. 1536-1540
    • Tani, K.1    Kurokawa, K.2    Nasu, M.3
  • 34
    • 0043030061 scopus 로고    scopus 로고
    • Detection of bacteria carrying the stx2 gene by in situ loop¬mediated isothermal amplification. Appl. Environ
    • Maruyama, F. et al. Detection of bacteria carrying the stx2 gene by in situ loop¬mediated isothermal amplification. Appl. Environ. Microbiol. 69, 5023-5028 (2003).
    • (2003) Microbiol , vol.69 , pp. 5023-5028
    • Maruyama, F.1
  • 35
    • 34247232247 scopus 로고    scopus 로고
    • Cloning and sequencing alleles of the KIR2DL4 gene from genomic DNA samples
    • Roberts, C.H., Madrigal, J.A. & Marsh, S.G. Cloning and sequencing alleles of the KIR2DL4 gene from genomic DNA samples. Tissue Antigens 69 (Suppl. 1): 88-91 (2007).
    • (2007) Tissue Antigens , vol.69 , pp. 88-91
    • Roberts, C.H.1    Madrigal, J.A.2    Marsh, S.G.3
  • 36
    • 0023183304 scopus 로고
    • Quantitative studies of human monokine-induced endothelial cell elongation
    • FitzGerald, O.M., Hess, E.V., Chance, A. & Highsmith, R.F. Quantitative studies of human monokine-induced endothelial cell elongation. J. Leukoc. Biol. 41, 421¬428 (1987).
    • (1987) J. Leukoc. Biol , vol.41 , pp. 421-428
    • Fitzgerald, O.M.1    Hess, E.V.2    Chance, A.3    Highsmith, R.F.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.