Exploring Protein Superstructures and Dynamics in Live Bacterial Cells Using Single-Molecule and Superresolution Imaging

Methods in Molecular Biology

Volumn 783, Issue , 2011, Pages 139-158

  Biteen, Julie S ^a   Shapiro, Lucy ^b   Moerner, W E ^b  

^a UNIVERSITY OF MICHIGAN   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

Caulobacter crescentus; Live cell imaging; Live cell PALM; Single molecule imaging; Superlocalization; Superresolution imaging

Indexed keywords

BACTERIAL PROTEIN;

ARTICLE; CAULOBACTER CRESCENTUS; CHEMISTRY; METABOLISM; METHODOLOGY; MOLECULAR IMAGING; THEORETICAL MODEL;

BACTERIAL PROTEINS; CAULOBACTER CRESCENTUS; MODELS, THEORETICAL; MOLECULAR IMAGING;

BACTERIA (MICROORGANISMS); CAULOBACTER VIBRIOIDES;

EID: 80054744163     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-282-3_8     Document Type: Chapter

References (38)

1. Ambrose W. P., Moerner W. E. (1991) Fluorescence spectroscopy and spectral diffusion of single impurity molecules in a crystal Nature 349, 225–7.
2. Thompson R. E., Larson D. R., Webb W. W. (2002) Precise nanometer localization analysis for individual fluorescent probes Biophys J 82, 2775–83.
3. Sch€utz G. J., Kada G., Pastushenko V. P., Schindler H. (2000) Properties of lipid micro- domains in a muscle cell membrane visualized by single molecule microscopy EMBO J 19, 892–901.
4. Sako Y., Minoghchi S., Yanagida T. (2000) Single-molecule imaging of EGFR signalling on the surface of living cells Nat Cell Biol 2, 168–72.
5. Moerner W. E. (2003) Optical measurements of single molecules in cells Trends Anal Chem 22, 544–8.
6. Vrljic M., Nishimura S. Y., Moerner W. E., McConnell H. M. (2005) Cholesterol Depletion Suppresses the Translational Diffusion of Class II Major Histocompatibility Complex Proteins in the Plasma Membrane Biophys J 88, 334–47.
7. Vrljic M., Nishimura S. Y., Brasselet S., Moerner W. E., McConnell H. M. (2002) Translational Diffusion of Individual Class II MHC Membrane Proteins in Cells Biophys J 83, 2681–92.
8. Lee H. D., Dubikovskaya E. A., Hwang H., Semyonov A. N., Wang H., Jones L. R., Twieg R. J., Moerner W. E., Wender P. A. (2008) Single-Molecule Motions of Oligoarginine Transporter Conjugates on the Plasma Membrane of Chinese Hamster Ovary Cells J Am Chem Soc 130, 9364–70.
9. Harms G. S., Cognet L., Lommerse P. H. M., Blab G. A., Schmidt T. (2001) Autofluores-cent Proteins in Single-Molecule Research: Applications to Live Cell Imaging Microscopy Biophys J 80, 2396–408.
10. Deich J., Judd E. M., McAdams H. H., Moerner W. E. (2004) Visualization of the movement of single histidine kinase molecules in live Caulobacter cells Proc Nat Acad Sci USA 101, 15921–6.
11. Kim S. Y., Gitai Z., Kinkhabwala A., Shapiro L., Moerner W. E. (2006) Single molecules of the bacterial actin MreB undergo directed treadmilling motion in Caulobacter crescentus Proc Nat Acad Sci USA 103, 10929–34.
12. Xie X. S., Choi P. J., Li G. W., Lee N. K., Lia G. (2008) Single-Molecule Approach to Molecular Biology in Living Bacterial Cells Annu Rev Biophys 37, 417–44.
13. Conley N. R., Biteen J. S., Moerner W. E. (2008) Cy3-Cy5 Covalent Heterodimers for Single-Molecule Photoswitching J Phys Chem B 112, 11878–80.
14. Bowman G. R., Comolli L. R., Zhu J., Eckart M., Koenig M., Downing K. H., Moerner W. E., Earnest T., Shapiro L. (2008) A Polymeric Protein Anchors the Chromosomal Ori-gin/ParB Complex at a Bacterial Cell Pole Cell 134, 945–55.
15. Biteen J. S., Thompson M. A., Tselentis N. K., Bowman G. R., Shapiro L., Moerner W. E. (2008) Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP Nat Methods 5, 947–9.
16. Moerner W. E., Fromm D. P. (2003) Methods of Single-Molecule Fluorescence Spectroscopy and Microscopy Rev Sci Instrum 74, 3597–619.
17. Betzig E., Patterson G. H., Sougrat R., Lind-wasser O. W., Olenych S., Bonifacino J. S., Davidson M. W., Lippincott-Schwartz J., Hess H. F. (2006) Imaging intracellular fluorescent proteins at nanometer resolution Science 313, 1642–5.
18. Rust M. J., Bates M., Zhuang X. (2006) Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) Nat Methods 3, 793–5.
19. Hess S. T., Girirajan T. P. K., Mason M. D. (2006) Ultra-high resolution imaging by fluorescence photoactivation localization microscopy Biophys J 91, 4258–72.
20. Thompson M. A., Biteen J. S., Lord S. J., Conley N. R., Moerner W. E. (2010) Molecules and Methods for Super-resolution Imaging Methods Enzymol 475, 27–59.
21. Wheeler R. T., Shapiro L. (1999) Differential localization of two histidine kinases controlling bacterial cell differentiation Mol Cell 4, 683–94.
22. Shapiro L., McAdams H., Losick R. (2002) Generating and exploiting polarity in bacteria Science 298, 1942–6.
23. Rudner D. Z., Pan Q., Losick R. M. (2002) Evidence that subcellular localization of a bacterial membrane protein is achieved by diffusion and capture Proc Nat Acad Sci USA 99, 8701–6.
24. Steinhauer J., Agha R., Pham T., Varga A. W., Goldberg M. B. (1999) The unipolar Shigella surface protein IcsA is targeted directly to the bacterial old pole: IcsP cleavage of IcsA occurs over the entire bacterial surface Mol Microbiol 32, 367–77.
25. Robbins J. R., Monack D., McCallum S. J., Vegas A., Pham E., Goldberg M. B., Theriot J. A. (2001) The making of a gradient: IcsA (VirG) polarity in Shigella flexneri Mol Microbiol 41, 861–72.
26. Gitai Z., Dye N., Shapiro L. (2004) An actin-like gene can determine cell polarity in bacteria Proc Nat Acad Sci USA 101, 8643–8.
27. Gitai Z., Dye N. A., Reisenauer A., Wachi M., Shapiro L. (2005) MreB actin-mediated segregation of a specific region of a bacterial chromosome Cell 120, 329–41.
28. Dye N. A., Pincus Z., Theriot J. A., Shapiro L., Gitai Z. (2005) Two independent spiral structures control cell shape in Caulobacter Proc Nat Acad Sci USA 102, 18608–13.
29. Van Den Ent E., Amos L. A., Lowe J. (2001) Prokaryotic origin of the actin cytoskelecton Nature 413, 39–44.
30. Figge R. M., Divakaruni A. V., Gober J. W. (2004) MreB, the cell shape-determining bacterial actin homologue, co-ordinates cell wall morphogenesis in Caulobacter crescentus Mol Microbiol 51, 1321–32.
31. Dickson R. M., Cubitt A. B., Tsien R. Y., Moerner W. E. (1997) On/Off Blinking and Switching Behavior of Single Green Fluorescent Protein Molecules Nature 388, 355–8.
32. Shroff H., Galbraith C. G., Galbraith J. A., Betzig E. (2008) Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics Nat Methods 5, 417–23.
33. Juette M. F., Gould T. J., Lessard M. D., Mlodzianoski M. J., Nagpure B. S., Bennett B. T., Hess S. T., Bewersdorf J. (2008) Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples. Nat Meth 5, 527–9.
34. Huang B., Wang W., Bates M., Zhuang X. (2008) Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy Science 319, 810–3.
35. Pavani S. R. P., Thompson M. A., Biteen J. S., Lord S. J., Liu N., Twieg R. J., Piestun R., Moerner W. E. (2009) Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function Proc Nat Acad Sci USA 106, 2995–9.
36. Shroff H., Galbraith C. G., Galbraith J. A., White H., Gillette J., Olenych S., Davidson M. W., Betzig E. (2007) Dual-color super-resolution imaging of genetically expressed probes within individual adhesion complexes Proc Nat Acad Sci USA 104, 20308–13.
37. Bates M., Huang B., Dempsey G. T., Zhuang X. (2007) Multicolor super-resolution imaging with photo-switchable fluorescent probes Science 317, 1749–53.
38. Vaziri A., Tang J., Shroff H., Shank C. V. (2008) Multilayer three-dimensional super resolution imaging of thick biological samples Proc Nat Acad Sci USA 105, 20221–6.