Temperature non-homogeneity in rapid airflow-based cycler significantly affects real-time PCR

BioTechniques

Volumn 33, Issue 3, 2002, Pages 508-512

  Zuna, Jan ^a   Muzikova, K ^a   Madzo, J ^a   Krejci, O ^a   Trka, J ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

AIRFLOW; ARTICLE; ASSAY; COOLING; FLUORESCENCE; GENE DELETION; GENE FUNCTION; GENE TARGETING; GENETIC TRANSCRIPTION; GENOME; HEATING; HYBRIDIZATION; POLYMERASE CHAIN REACTION; TEMPERATURE DEPENDENCE; AIR; EQUIPMENT; EVALUATION; GENETICS; INSTRUMENTATION; METHODOLOGY; QUALITY CONTROL; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TEMPERATURE;

BETA 2 MICROGLOBULIN; DNA; PROTEIN P53;

AIR MOVEMENTS; BETA 2-MICROGLOBULIN; DNA; EQUIPMENT FAILURE ANALYSIS; POLYMERASE CHAIN REACTION; QUALITY CONTROL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TEMPERATURE; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0036752145     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02333bm09_11837a     Document Type: Article

References (1)

1. Wilhelm, J., M. Hahn, and A. Pingoud. 2000. Influence of DNA target melting behavior on real-time PCR quantification. Clin. Chem. 46:1738-1743.