Sample number and denaturation time are crucial for the accuracy of capillary-based lightcyclers [6]

Clinical Chemistry

Volumn 53, Issue 7, 2007, Pages 1392-1394

  Von Kanel, Thomas ^a   Adolf, Florentin ^a   Schneider, Mircea ^a   Sanz, Javier ^a   Gallati, Sabina ^a,b  

^a UNIVERSITY OF BERN   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA;

ACCURACY; BIOMEDICAL TECHNOLOGY ASSESSMENT; DNA DENATURATION; DNA DETERMINATION; GENE AMPLIFICATION; LETTER; SAMPLING; TEMPERATURE DEPENDENCE; TIME;

ADENOSINE TRIPHOSPHATASES; AUTOANTIGENS; CATION TRANSPORT PROTEINS; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; RIBONUCLEOPROTEINS, SMALL NUCLEAR; SOFTWARE; TIME FACTORS;

EID: 34347380542     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2007.086249     Document Type: Letter

References (5)

1. Wilhelm J, Hahn M, Pingoud A. Influence of DNA target melting behavior on real-time PCR quantification. Clin Chem 2000;46:1738-43.
2. Zuna J, Muzikova K, Madzo J, Krejci O, Trka J. Temperature non-homogeneity in rapid airflow-based cycler significantly affects real-time PCR. Biotechniques 2002;33:508-12.
3. Schneider M, Joncourt F, Sanz J, von Kanel T, Gallati S. Detection of exon deletions within an entire gene (CFTR) by relative quantification on the LightCycler. Clin Chem 2006;52:2005-12.
4. Wittwer CT. Rapid cycle real-time PCR: methods and applications. In: Meuer S, Wittwer CT, Nakagawara K, eds. Rapid Cycle Real-Time PCR. Berlin: Springer-Verlag, 2001:1-8.
5. Pierce AL, Dickey JT, Larsen DA, Fukada H, Swanson P, Dickhoff WW. A quantitative real-time RT-PCR assay for salmon IGF-I mRNA, and its application in the study of GH regulation of IGF-I gene expression in primary culture of salmon hepatocytes. Gen Comp Endocrinol 2004;135:401-11.