Improvement of the expression and purification of Mycobacterium tuberculosis arylamine N-acetyltransferase (TBNAT) a potential target for novel anti-tubercular agents

Protein Expression and Purification

Volumn 80, Issue 2, 2011, Pages 246-252

  Abuhammad, Areej ^a   Lack, Nathan ^a   Schweichler, Judith ^a   Staunton, David ^b   Sim, Robert B ^a   Sim, Edith ^a,c  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c KINGSTON UNIVERSITY   (United Kingdom)

Author keywords

Arylamine N acetyltransferase; Latent tuberculosis; pVLT3 Plasmid; Thermal shift assay

Indexed keywords

ARYLAMINE ACETYLTRANSFERASE; BACTERIAL PROTEIN; BUFFER; HISTIDINE; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT PROTEIN;

ARTICLE; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVATION; ENZYME ASSAY; ENZYMOLOGY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; MYCOBACTERIUM TUBERCULOSIS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN STABILITY; SOLUBILITY; TEMPERATURE;

ARYLAMINE N-ACETYLTRANSFERASE; BACTERIAL PROTEINS; BUFFERS; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME ASSAYS; ESCHERICHIA COLI; HISTIDINE; ISOPROPYL THIOGALACTOSIDE; MOLECULAR WEIGHT; MYCOBACTERIUM TUBERCULOSIS; PLASMIDS; PROTEIN STABILITY; RECOMBINANT PROTEINS; SOLUBILITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TEMPERATURE;

ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 80054067439     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.06.021     Document Type: Article

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