Comparative mycobacterial genomics

Current Opinion in Microbiology

Volumn 1, Issue 5, 1998, Pages 567-571

  Cole, Stewart T ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

MYCOBACTERIUM LEPRAE; MYCOBACTERIUM TUBERCULOSIS;

BACTERIAL GENOME; GENETICS; MYCOBACTERIUM LEPRAE; MYCOBACTERIUM TUBERCULOSIS; REVIEW;

GENOME, BACTERIAL; MYCOBACTERIUM LEPRAE; MYCOBACTERIUM TUBERCULOSIS;

EID: 0032173615     PISSN: 13695274     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1369-5274(98)80090-8     Document Type: Article

References (27)

1. Cole ST, Brosch R, Parkhill J, Gamier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE III et al.: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 1998, 393:537-544. The authors describe the complete genome sequence of the most widely used strain of M. tuberculosis H37Rv and interpret the genomic message using various bioinformalic methods. The main themes addressed are slow growth, dormancy, genetic homogeneity, biosynthesis of the cell envelope and the PE and PPE gene families. The possibility that PE and PPE proteins may be sources of antigenic variation or inhibitors of antigen presentation is discussed.
2. Brosch R, Gordon SV, Billault A, Garnier T, Eiglmeier K, Soravito C, Barrell BG, Cole ST: Use of a Mycobacterium tuberculosis H37Rv Bacterial Artificial Chromosome (BAC) library for genome mapping, sequencing and comparative genomics. Infect Immun 1998, 66:2221-2229. This paper describes the first successful application of BACs in bacterial genomics and shows how complete genome coverage could be obtained in contrast to the situation with cosmids. The complete genome can be represented by 68 BACs. An application in comparative genomics is presented and a 12.7 kb region of difference between the chromosomes of M. tuberculosis, M. bovis and M. bovis BCG characterised.
3. Hacker J, Blum OG, Muhldorfer I, Tschape H: Pathogenicity islands of virulent bacteria: structure, function and impact. Mol Microbiol 1997, 23:1089-1097.
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5. Pelicic V, Jackson M, Reyrat JM, Jacobs WR Jr, Gicquel B, Guilhot C: Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis. Proc Natl Acad Sci USA 1997, 94:10955-10960. This publication describes the development of a highly efficient vector system that greatly facilitates gene replacements in mycobacteria. This system is certain to find widespread application. Preliminary results of its use as a vehicle for transposon delivery are presented.
6. Bardarov S, Kriakov J, Carriere C, Yu S, Vaamonde C, McAdam R, Bloom BR, Hatfull GR, Jacobs JWR: Conditionally replicating mycobacteriophages: a system for transposon delivery to Mycobacterium tuberculosis. Proc Natl Acad Sci USA 1997, 94:10961-10966. The authors describe the construction of a synthetic transposon and its application as an insertional mutagen in M. tuberculosis. The results are interpreted in the context of the genome sequence and suggest that this approach could be very useful for identifying and inactivating nonessential genes. Some bias in the sites of insertion was seen that reflects the locations of the endogenous IS (insertion sequence) elements in the genome.
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11. Cole ST, Barrell BG: Analysis of the genome of Mycobacterium tuberculosis H37Rv. In Genetics and Tuberculosis (Novartis Foundation Symposium 217). Edited by Chadwick DJ, Cardew G. Chichester: John Wiley; 1998:160-172.
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17. Poulet S, Cole ST: Repeated DNA sequences in mycobacteria. Arch Microbiol 1995, 163:79-86.
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20. Fsihi H, De Rossi E, Salazar L, Cantoni R, Labò M, Riccardi G, Takiff HE, Eiglmeier K, Bergh S, Cole ST: Gene arrangement and organisation in a ∼76 kilobase fragment encompassing the oriC region of the chromosome of Mycobacterium leprae. Microbiology 1996, 142:3147-3161.
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