Purification of SUMO-1 modified IκBα and complex formation with NF-κB

Protein Expression and Purification

Volumn 80, Issue 2, 2011, Pages 211-216

  Lens, Zoé ^a   Dewitte, Frédérique ^a   Van Lint, Carine ^b   De Launoit, Yvan ^c   Villeret, Vincent ^a   Verger, Alexis ^a  

^a CNRS   (France)

^b UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^c UNIV LILLE   (France)

Author keywords

I B ; NF B; On column tag cleavage; SUMO; Sumoylation

Indexed keywords

I KAPPA B; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; NF KAPPAB INHIBITOR ALPHA; NF-KAPPAB INHIBITOR ALPHA; NFKB1 PROTEIN, HUMAN; RECOMBINANT PROTEIN; SUMO 1 PROTEIN; SUMO1 PROTEIN, HUMAN; TRANSCRIPTION FACTOR RELA;

AFFINITY CHROMATOGRAPHY; ARTICLE; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; SUMOYLATION; ULTRAFILTRATION;

CATALYTIC DOMAIN; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; I-KAPPA B PROTEINS; NF-KAPPA B P50 SUBUNIT; PROTEIN INTERACTION MAPPING; RECOMBINANT PROTEINS; SUMO-1 PROTEIN; SUMOYLATION; TRANSCRIPTION FACTOR RELA; ULTRAFILTRATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 80054062402     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.06.009     Document Type: Article

References (26)

1. M. Hochstrasser Origin and function of ubiquitin-like proteins Nature 458 2009 422 429
2. J.R. Gareau, and C.D. Lima The SUMO pathway: emerging mechanisms that shape specificity, conjugation and recognition Nat. Rev. Mol. Cell Biol. 11 2010 861 871
3. R. Geiss-Friedlander, and F. Melchior Concepts in sumoylation: a decade on Nat. Rev. Mol. Cell Biol. 8 2007 947 956 (Pubitemid 350176509)
4. K.A. Wilkinson, and J.M. Henley Mechanisms, regulation and consequences of protein SUMOylation Biochem. J. 428 2010 133 145
5. A. Werner, M.C. Moutty, U. Moller, and F. Melchior Performing in vitro sumoylation reactions using recombinant enzymes Methods Mol. Biol. 497 2009 187 199
6. A.A. Yunus, and C.D. Lima Purification of SUMO conjugating enzymes and kinetic analysis of substrate conjugation Methods Mol. Biol. 497 2009 167 186
7. R.T. Hay SUMO: a history of modification Mol. Cell 18 2005 1 12 (Pubitemid 40444643)
8. A. Verger, J. Perdomo, and M. Crossley Modification with SUMO: a role in transcriptional regulation EMBO Rep. 4 2003 137 142 (Pubitemid 36322153)
9. M.A. Langereis, G. Rosas-Acosta, K. Mulder, and V.G. Wilson Production of sumoylated proteins using a baculovirus expression system J. Virol. Methods 139 2007 189 194 (Pubitemid 46074180)
10. M. Mencia, and V. de Lorenzo Functional transplantation of the sumoylation machinery into Escherichia coli Protein Expr. Purif. 37 2004 409 418 (Pubitemid 39187979)
11. Y. Uchimura, M. Nakamura, K. Sugasawa, M. Nakao, and H. Saitoh Overproduction of eukaryotic SUMO-1- and SUMO-2-conjugated proteins in Escherichia coli Anal. Biochem. 331 2004 204 206 (Pubitemid 38881579)
12. Y. Uchimura, M. Nakao, and H. Saitoh Generation of SUMO-1 modified proteins in E. coli: towards understanding the biochemistry/structural biology of the SUMO-1 pathway FEBS Lett. 564 2004 85 90 (Pubitemid 38490704)
13. H. Saitoh, J. Uwada, and K. Azusa Strategies for the expression of SUMO-modified target proteins in Escherichia coli Methods Mol. Biol. 497 2009 211 221
14. P. Knipscheer, H. Klug, T.K. Sixma, and A. Pichler Preparation of sumoylated substrates for biochemical analysis Methods Mol. Biol. 497 2009 201 210
15. A. Chariot, F. Princen, J. Gielen, M.P. Merville, G. Franzoso, K. Brown, U. Siebenlist, and V. Bours IκBα enhances transactivation by the HOXB7 homeodomain-containing protein J. Biol. Chem. 274 1999 5318 5325 (Pubitemid 29109173)
16. A. Dekoninck, C. Calomme, S. Nizet, Y. de Launoit, A. Burny, J. Ghysdael, and C. Van Lint Identification and characterization of a PU 1/Spi-B binding site in the bovine leukemia virus long terminal repeat Oncogene 22 2003 2882 2896 (Pubitemid 36648915)
17. Z. Lens, F. Dewitte, D. Monté, J.L. Baert, C. Bompard, M. Sénéchal, C. Van Lint, Y. de Launoit, V. Villeret, and A. Verger Solution structure of the N-terminal transactivation domain of ERM modified by SUMO-1 Biochem. Biophys. Res. Commun. 399 2010 104 110
18. J.M. Desterro, M.S. Rodriguez, and R.T. Hay SUMO-1 modification of IκBα inhibits NF-κB activation Mol. Cell 2 1998 233 239 (Pubitemid 128373648)
19. T. Huxford, D.B. Huang, S. Malek, and G. Ghosh The crystal structure of the IκBα/NF-κB complex reveals mechanisms of NF-κB inactivation Cell 95 1998 759 770 (Pubitemid 29014456)
20. M.D. Jacobs, and S.C. Harrison Structure of an IκBα/NF- κB complex Cell 95 1998 749 758
21. T. Huxford, S. Malek, and G. Ghosh Preparation and crystallization of dynamic NF-κB-IκBα complexes J. Biol. Chem. 275 2000 32800 32806
22. E. Jaffray, K.M. Wood, and R.T. Hay Domain organization of Iκ B alpha and sites of interaction with NF-κB p65 Mol. Cell Biol. 15 1995 2166 2172
23. C.H. Croy, S. Bergqvist, T. Huxford, G. Ghosh, and E.A. Komives Biophysical characterization of the free IκBα ankyrin repeat domain in solution Protein Sci. 13 2004 1767 1777 (Pubitemid 38822116)
24. R.T. Hay, L. Vuillard, J.M. Desterro, and M.S. Rodriguez Control of NF-κB transcriptional activation by signal induced proteolysis of I κB alpha Philos. Trans. R. Soc. Lond. B. Biol. Sci. 354 1999 1601 1609 (Pubitemid 29464091)
25. M.P. Kracklauer, and C. Schmidt At the crossroads of SUMO and NF-κB Mol. Cancer 2 2003 39
26. C. Culver, A. Sundqvist, S. Mudie, A. Melvin, D. Xirodimas, and S. Rocha Mechanism of hypoxia-induced NF-κB Mol. Cell Biol. 30 2010 4901 4921