Preparation of sumoylated substrates for biochemical analysis

Methods in Molecular Biology

Volumn 497, Issue , 2009, Pages 201-210

  Knipscheer, Puck ^a   Klug, Helene ^b   Sixma, Titia K ^c   Pichler, Andrea ^d  

^a HARVARD MEDICAL SCHOOL   (United States)

^b MEDICAL UNIVERSITY OF VIENNA   (Austria)

^c NETHERLANDS CANCER INSTITUTE   (Netherlands)

^d NONE

Author keywords

E2 25K; In vitro sumoylation assay with recombinant enzymes; Protein purification; SUMO

Indexed keywords

PROTEIN; RECOMBINANT PROTEIN; SUMO PROTEIN; UBIQUITIN CONJUGATING ENZYME;

BIOCHEMISTRY; DIAGNOSIS, MEASUREMENT AND ANALYSIS; ENZYME SPECIFICITY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PROTEIN PROCESSING; REVIEW;

BIOCHEMISTRY; HUMANS; LABORATORY TECHNIQUES AND PROCEDURES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEINS; RECOMBINANT PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SUBSTRATE SPECIFICITY; UBIQUITIN-CONJUGATING ENZYMES;

EID: 59249093684     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-566-4_13     Document Type: Article

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