Strategies for the expression of SUMO-modified target proteins in Escherichia coli

Methods in Molecular Biology

Volumn 497, Issue , 2009, Pages 211-221

  Saitoh, Hisato ^a   Uwada, Junsuke ^a   Azusa, Kawasaki ^a  

^a KUMAMOTO UNIVERSITY   (Japan)

Author keywords

Affinity purification; In vivo sumoylation; Plasmid compatibility; Protein expression in E. coli; SUMO conjugation pathway

Indexed keywords

ESCHERICHIA COLI; MAMMALIA;

HYBRID PROTEIN; PROTEIN; SUMO PROTEIN;

ALGORITHM; BACTERIUM TRANSFORMATION; BIOLOGICAL MODEL; DIAGNOSIS, MEASUREMENT AND ANALYSIS; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MOLECULAR CLONING; PHYSIOLOGY; PROTEIN PROCESSING; REVIEW;

ALGORITHMS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; LABORATORY TECHNIQUES AND PROCEDURES; MICROBIOLOGICAL TECHNIQUES; MODELS, BIOLOGICAL; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEINS; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; TRANSFORMATION, BACTERIAL;

EID: 59249096264     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-566-4_14     Document Type: Article

References (8)

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