Selection of reference genes for qRT-PCR in high fat diet-induced hepatic steatosis mice model

Molecular Biotechnology

Volumn 48, Issue 3, 2011, Pages 255-262

  Xu, Lingyan ^a   Ma, Xinran ^a   Cui, Bin ^a   Li, Xiaoying ^a   Ning, Guang ^a   Wang, Shu ^a  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Gene expression; Hepatic steatosis; Housekeeping genes; Normalization; Quantitative real time PCR

Indexed keywords

CONSTANT LEVEL; CROSS VALIDATION; EXPERIMENTAL CONDITIONS; GENE EXPRESSION ANALYSIS; HEPATIC STEATOSIS; HOUSEKEEPING GENE; INTERNAL CONTROLS; INTRINSIC MECHANISMS; MICE MODELS; NORMALIZATION; OBESE MICE; Q-RT-PCR; QUANTITATIVE REAL TIME PCR;

MAMMALS; NUTRITION; POLYMERASE CHAIN REACTION;

GENE EXPRESSION;

PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; STEROL REGULATORY ELEMENT BINDING PROTEIN 1C; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE;

ALGORITHM; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DISEASE COURSE; FATTY LIVER; GENE EXPRESSION PROFILING; HISTOLOGY; HOUSEKEEPING GENE; LIPID DIET; LIVER PARENCHYMA; MOUSE; NONHUMAN; OBESITY; QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; ANIMAL; C57BL MOUSE; CYTOCHEMISTRY; ENZYMOLOGY; FAT INTAKE; GENETIC MARKER; GENETICS; METABOLISM; METHODOLOGY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD;

MUS;

ANIMALS; DIETARY FATS; FATTY LIVER; GENE EXPRESSION PROFILING; GENETIC MARKERS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); HISTOCYTOCHEMISTRY; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MICE; MICE, INBRED C57BL; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 80054034726     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-010-9366-2     Document Type: Article

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