A flexible approach to studying post-transcriptional gene regulation in stably transfected mammalian cells

Molecular Biotechnology

Volumn 48, Issue 3, 2011, Pages 210-217

  Nichols, Ralph C ^a,b   Botson, John ^b   Wang, Xiao Wei ^b   Hamilton, B Jonell ^b   Collins, Jane E ^b   Uribe, Victoria ^b   Brooks, Seth A ^a,b   Zan, Moe ^b   Rigby, William F C ^b  

^a VA MEDICAL CENTER   (United States)

^b DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

30 UTR; ARE; mRNA turnover; TNF; Translation

Indexed keywords

30-UTR; ARE; CIS-ACTING ELEMENT; MAMMALIAN CELLS; MRNA TURNOVER; POST-TRANSCRIPTIONAL GENE REGULATION; POST-TRANSCRIPTIONAL REGULATIONS; REGULATORY FUNCTIONS; REPORTER GENE; SERUM STARVATION; TECHNICAL LIMITATIONS; TNF;

GENES; MAMMALS;

TRANSCRIPTION;

COMPLEMENTARY DNA; TETRACYCLINE; TUMOR NECROSIS FACTOR; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

3' UNTRANSLATED REGION; ARTICLE; CHIMERA; CONTROLLED STUDY; GENE SEQUENCE; GENETIC TRANSFECTION; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; POSTTRANSCRIPTIONAL GENE SILENCING; REPORTER GENE; BIOLOGICAL MODEL; BIOSYNTHESIS; CELL STRAIN HEK293; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; MOLECULAR GENETICS; REGULATORY SEQUENCE; STATISTICAL ANALYSIS;

MAMMALIA;

3' UNTRANSLATED REGIONS; BASE SEQUENCE; DATA INTERPRETATION, STATISTICAL; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; MODELS, GENETIC; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; REGULATORY ELEMENTS, TRANSCRIPTIONAL; RNA, MESSENGER; TUMOR NECROSIS FACTOR-ALPHA;

EID: 80054034516     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-010-9360-8     Document Type: Article

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