Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm

Journal of Biomedical Optics

Volumn 16, Issue 9, 2011, Pages

  Li, David D U ^a,b   Arlt, Jochen ^b   Tyndall, David ^b   Walker, Richard ^b   Richardson, Justin ^b,c   Stoppa, David ^d   Charbon, Edoardo ^e   Henderson, Robert K ^b  

^a UNIVERSITY OF SUSSEX   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c STMicroelectronics Ltd Bristol   (United Kingdom)

^d FONDAZIONE BRUNO KESSLER   (Italy)

^e Smart Optical Sensors and Interfaces ^*  (Netherlands)

Author keywords

Center of mass; Fluorescence lifetime imaging microscopy; Photon counting; complementary metal oxide semiconductor; Single photon avalanche diode; Time resolved imaging

Indexed keywords

CENTER-OF-MASS; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; PHOTON COUNTING COMPLEMENTARY METAL-OXIDE-SEMICONDUCTOR; SINGLE PHOTON AVALANCHE DIODE; TIME RESOLVED IMAGING;

ALGORITHMS; DIELECTRIC DEVICES; DIODES; FLUORESCENCE; FREQUENCY CONVERTERS; LEAST SQUARES APPROXIMATIONS; MAXIMUM LIKELIHOOD ESTIMATION; PARTICLE BEAMS; PHOTONS; TABLE LOOKUP;

AVALANCHE DIODES;

MICROSPHERE; RHODAMINE; RHODAMINE 6G;

ALGORITHM; ARTICLE; DRUG ANTAGONISM; EQUIPMENT DESIGN; FLUORESCENCE MICROSCOPY; IMAGE PROCESSING; INSTRUMENTATION; METHODOLOGY; PHOTON; REGRESSION ANALYSIS; SEMICONDUCTOR; VIDEO MICROSCOPY;

ALGORITHMS; EQUIPMENT DESIGN; IMAGE PROCESSING, COMPUTER-ASSISTED; LEAST-SQUARES ANALYSIS; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; MICROSPHERES; PHOTONS; RHODAMINES; SEMICONDUCTORS;

EID: 80053940190     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.3625288     Document Type: Article

References (32)

1. S. Cova, M. Ghioni, A. Lacaita, C. Samori, and F. Zappa, "Avalanche photodiodes and quenching circuits for single-photon detection," Appl. Opt. 35, 1956-1976 (1996).
2. S. Cova, M. Ghioni, A. Lotito, I. Rech, and F. Zappa, "Evolution and prospects for single-photon avalanche diodes and quenching circuits," /. Mod. Opt. 51, 1267-1288 (2004).
3. L.-Q. Li and L. M. Davis, "Single photon avalanche diode for single molecule detection," Rev. Sci Instrum. 64, 1524-L529 (1993).
4. H. Yang, G. Luo, P. Karnchanaphanurach, T.-M. Louie, I. Rech, S. Cova, L. Xun, and X. S. Xie, "Protein conformational dynamics probed by single-molecule electron transfer," Science 10, 262-266 (2003).
5. C. Niclass, A. Rochas, P. A. Besse, and E. Charbon, "Design and characterization of a CMOS 3-D image sensor based on single photon avalanche diodes," IEEE J. Solid-State Circuits 40, 1847-1853 (2005).
6. D. Stoppa, D. Mosconi, L. Pancheri, and L. Gonzo, "Single-photon avalanche diode CMOS sensor for time-resolved fluorescence measurements," IEEE Sens. J. 9, 1084-1090 (2009).
7. D. E. Schwartz, P. Gong, and K. L. Shepard, "Time-resolved Försterresonance-energy-transfer DNA assay on an active CMOS microarray," Biosens. Bioelectron. 24, 383-390 (2008).
8. B. R. Bae, K. J.-B. Yang, J. McKendry, Z. Gong, D. Renshaw, J. M. Girkin, E. Gu, M. D. Dawson, and R. K. Henderson, "A vertically integrated CMOS microsystem for time-resolved fluorescence analysis," IEEE Trans. Biomed. Circ. Syst. 4, 437-444 (2010).
9. M. Gersbach, J. Richardson, E. Mazaleyrat, S. Hardilher, C. Niclass, R. Henderson, L. Grant, and E. Charbon, "A low-noise single-photon detector implemented in a 130 nm CMOS imaging process," Solid-State Electron. 53, 803-808 (2009).
10. J. A. Richardson, L. A. Grant, and R. K. Henderson, "Low dark count single-photon avalanche diode structure compatible with standard nanometer scale CMOS technology," IEEE Photon. Technol. Lett. 21, 1020-1022 (2009).
11. R. K. Henderson, E. A. G. Webster, R. Walker, J. A. Richardson, and L. A. Grant, "A 3×3, 5 mm pitch, 3-transistor single photon avalanche diode array with integrated 11 V bias generation in 90 nm CMOS technology," in Proc. 2010 IEEE Int. Electron Devices Meeting (IEDM), pp. 14.2, Dec. 6-8, San Francisco, CA (2010).
12. E. A. G. Webster, J. A. Richardson, L. A. Grant, D. Renshaw, and R. K. Henderson, "An infra-red sensitive, low noise, single-photon avalanche diode in 90 nm CMOS," International Image Sensor Workshop (IISW), Hokkaido, Japan, 8-11 June 2011.
13. R. A. Colyer, G. Scalia, I. Rech, A. Gulinatti, M. Ghioni, S. Cove, S. Weiss, and X. Michalet, "High-throughput FCS using an LCOS spatial light modulator and an 8x1 SPAD array," Biomed Opt. Express 1, 1408-1430 (2010).
14. D.-U. Li, B. Rae, R. Andrews, J. Arlt, and R. Henderson, "Hardware implementation algorithm and error analysis of high-speed fluorescence lifetime sensing systems using center-of-mass method," J. Biomed. Opt. 15, 017006 (2010).
15. P. Hall and B. Selinger, "Better estimates of exponential decay parameters," J. Phys. Chem. 85, 2941-2946 (1981).
16. D. S. Elson, I. Munro, J. Requejo-Isidro, J. McGinty, C. Dunsby, N. Galletly, G. W Stamp, M. A. A. Neil, M. J. Lever, P. A. Kellett, A. Dymoke-Bradshaw, J. Hares, and P. M. W. French, "Real-time time-domain fluorescence lifetime imaging including single-shot acquisition with a segmented optical image intensifier,"New J. Phys. 6, 1-13 (2004).
17. M. Elangovan, R. N. Day, and A. Periasamy, "Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell," J. Microsc. 205, 3-14 (2002).
18. G.I. Redford and R. M. Clegg, "Real-time fluorescence lifetime imaging and FRET using fast gated intensifiers," in Molecular Imaging: FRET Microscopy and Spectroscopy, A. Periasamy and R. N. Day, Eds., pp. 193-226, Oxford University, New York (2005).
19. A. Leray, F. B. Riquet, E. Richard, C. Spriet, D. Trinel, and L. Héliot, "Optimized protocol of a frequency domain fluorescence lifetime imaging microscope for FRET measurements," Microsc. Res. Tech. 72, 371-379 (2009).
20. A. C. Mitchell, J. E. Wall, J. G. Murray, and C. G. Morgan, "Direct modulation of the effective sensitivity of a CCD detector: a new approach to time-resolved fluorescence imaging," J. Microsc. 206, 225-232 (2002).
21. J. Richardson, R. Walker, L. Grant, D. Stoppa, F. Borghetti, E. Charbon, M. Gersbach, and R. Henderson, "A 32×32 50 ps resolution 10 bit time to digital converter array in 130 nm CMOS for time correlated imaging," in Proc. IEEE Custom Integrated Circuits Conf. (CICC), pp. 77-80, Sept. 13-16, San Jose, CA (2009).
22. Spartan-III FPGA Family Data Sheet: Complete Data Sheet, Datasheet DS099 (v2.5), Xilinx, Inc., San Jose, CA (2009).
23. R. M. Ballew and J. N. Demas, "An error analysis of the rapid lifetime determination method for the evaluation of single exponential decays," Anal. Chem. 61, 30-33 (1989).
24. A. V. Agronskaia, L. Tertoolen, and H. C. Gerritsen, "Fast fluorescence lifetime imaging of calcium in living cells," J. Biomed. Opt. 9, 1230-1237 (2004).
25. D.-U. Li, B. Rae, E. Bonnist, D. Renshaw, and R. Henderson, "Onchip fluorescence lifetime extraction using synchronous gating scheme: theoretical error analysis and practical implementation," in Proc. Int. Conf. Bio-inspired Systems and Signal processing, Funchal, Portugal, pp. 171-176 (2008), INSTICC, Lisbon, Portugal.
26. C. Moore, S. P. Chan, J. N. Demas, and B. A. Degraff, "Comparison of methods for rapid evaluation of lifetime of exponential decays," Appl. Spectrosc. 58, 603-607 (2004).
27. G. Giraud, H. Schulze, D.-U. Li, T. T. Bachmann, J. Crain, D. Tyndall, J. Richardson, R. Walker, D. Stoppa, E. Charbon, R. Henderson, and J. Arlt, "Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager," Biomed Opt. Express 1, 1302-1308 (2010).
28. G. Giraud, H. Schulze, T. T. Bachmann, C. J. Campbell, A. R. Mount, P. Ghazal, M. R. Khondoker, A. J. Ross, S. W J. Ember, I. Ciani, C. Tlili, A. J. Walton, J. G. Terry, and J. Crain, "Fluorescence lifetime imaging of quantum dot labeled DNA microarrays," Int. J. Mol. Sci. 10, 1930-1941 (2009).
29. A. Draaijer, R. Sanders, and H. C. Gerritsen, "Fluorescence lifetime imaging, a new tool in confocal microscopy," in Handbook of Biological Confocal Microscopy, J. Pawley, Ed., pp. 491-505, Plenum, New York (1995).
30. S. Donati, G. Martini, and M. Norgia, "Microconcentrators to recover fill-factor in image photodetectors with pixel on-board processing circuits," Opt. Express 15, 18066-18075 (2007).
31. F. G. E. Cremazy, E. M. M. Manders, P. I. H. Bastiaens, G. Kramer, G. L. Hager, E. B. Van Munster, P. J. Verschure, T. W. J. Gadella Jr., and R. Van Driel, "Imaging in situ protein-DNA interactions in the cell nucleus using FRET-FLIM," Exp. Cell Res. 309, 390-396 (2005).
32. D. Elson, R. Requejo-Isidro, I. Munro, F. Reavell, J. Siegel, K. Suhling, P. Tadrous, R. Benninger, P. Lanigan, J. McGinty, C. Talbot, B. Treanor, S. Webb, A. Sandison, A. Wallace, D. Davis, J. Lever, M. Neil, D. Phillips, G. Stamp, and P. French, "Time-domain fluorescence lifetime imaging applied to biological tissue," Photochem. Photobiol. Sci. 3, 795-801 (2004).