Coordinated sumoylation and ubiquitination modulate EGF induced EGR1 expression and stability

PLoS ONE

Volumn 6, Issue 10, 2011, Pages

  Manente, Arcangela Gabriella ^a   Pinton, Giulia ^a   Tavian, Daniela ^b   Lopez Rodas, Gerardo ^c   Brunelli, Elisa ^a   Moro, Laura ^a  

^a UNIVERSITY OF EASTERN PIEDMONT   (Italy)

^b UNIVERSITÀ CATTOLICA DEL SACRO CUORE   (Italy)

^c UNIVERSITY OF VALENCIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BENZYLOXYCARBONYLLEUCYLLEUCYLLEUCINAL; EARLY GROWTH RESPONSE FACTOR 1; EPIDERMAL GROWTH FACTOR; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; PROTEIN UBC9; SUMO 1 PROTEIN; EGR1 PROTEIN, HUMAN; PROTEASOME; UBIQUITIN CONJUGATING ENZYME; UBIQUITIN CONJUGATING ENZYME UBC9; UBIQUITIN-CONJUGATING ENZYME UBC9;

ARTICLE; CELL LEVEL; CONTROLLED STUDY; EGR1 GENE; ENZYME PHOSPHORYLATION; GENE; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; GENETIC STABILITY; HUMAN; HUMAN CELL; NONHUMAN; PROTEIN DEGRADATION; PROTEIN FUNCTION; PROTEIN METABOLISM; SUMOYLATION; TRANSCRIPTION REGULATION; TRANSIENT TRANSFECTION; UBIQUITINATION; CELL LINE; CHEMISTRY; DRUG EFFECT; ENZYME ACTIVATION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; PROTEIN STABILITY; TIME;

CELL LINE; EARLY GROWTH RESPONSE PROTEIN 1; ENZYME ACTIVATION; EPIDERMAL GROWTH FACTOR; GENE EXPRESSION REGULATION; HUMANS; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN STABILITY; PROTEOLYSIS; SUMO-1 PROTEIN; SUMOYLATION; TIME FACTORS; TRANSCRIPTION, GENETIC; UBIQUITIN-CONJUGATING ENZYMES;

EID: 80053617325     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0025676     Document Type: Article

References (42)

1. Sukhatme VP, (1990) Early transcriptional events in cell growth: the Egr family. J Am Soc Nephrol 1 (6): 859-66 Review.
2. Sukhatme VP, (1991) The Egr family of nuclear signal transducers. Am J Kidney Dis 17 (6): 615-8 Review.
3. Cao X, Mahendran R, Guy GR, Tan YH, (1993) Detection and characterization of cellular EGR-1 binding to its recognition site. J Biol Chem 268: 16949-16957.
4. Swirnoff AH, Apel ED, Svaren J, Sevetson BR, Zimonjic DB, et al. (1998) NAB1, a corepressor of NGFI-A (Egr-1), contains an active transcriptional repression domain. Mol Cell Biol 18 (1): 512-24.
5. Svaren J, Sevetson BR, Apel ED, Zimonjic DB, Popescu NC, et al. (1996) NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli. J Mol Cell Biol 16 pp. 3545-53.
6. Teutschbein J, Haydn JM, Samans B, Krause M, Eilers M, et al. (2010) Gene expression analysis after receptor tyrosine kinase activation reveals new potential melanoma proteins. BMC Cancer 10: 386.
7. Gousseva N, Kugathasan K, Chesterman CN, Khachigian LM, (2001) Early growth response factor-1 mediates insulin-inducible vascular endothelial cell proliferation and regrowth after injury. J Cell Biochem 81 (3): 523-34.
8. Mayer SI, Rössler OG, Endo T, Charnay P, Thiel GJ, (2009) Epidermal-growth-factor-induced proliferation of astrocytes requires Egr transcription factors. Cell Sci 122 (Pt 18): 3340-50.
9. Blume A, Torner L, Liu Y, Subburaju S, Aguilera G, et al. (2009) Prolactin induces Egr-1 gene expression in cultured hypothalamic cells and in the rat hypothalamus. Brain Res 1302: 34-41.
10. Huang RP, Adamson ED, (1995) A biological role for Egr-1 in cell survival following ultra-violet irradiation. Oncogene 10 (3): 467-75.
11. Copland IB, Post M, (2007) Stretch-activated signaling pathways responsible for early response gene expression in fetal lung epithelial cells. J Cell Physiol 210 (1): 133-43.
12. Liu C, Calogero A, Ragona G, Adamson E, Mercola D, (1996) EGR-1, the reluctant suppression factor: EGR-1 is known to function in the regulation of growth, differentiation, and also has significant tumor suppressor activity and a mechanism involving the induction of TGF-beta1 is postulated to account for this suppressor activity. Crit Rev Oncog 7 (1-2): 101-25 Review.
13. Liu C, Rangnekar VM, Adamson E, Mercola D, (1998) Suppression of growth and transformation and induction of apoptosis by EGR1. Cancer Gene Ther 5: 3-28.
14. Yu J, Baron V, Mercola D, Mustelin T, Adamson ED, (2007) A network of p73, p53 and Egr1 is required for efficient apoptosis in tumor cells. Cell Death Differ 14: 436-446.
15. Bae SK, Bae MH, Ahn MY, Son MJ, Lee YM, et al. (1999) Egr-1 mediates transcriptional activation of IGF-II gene in response to hypoxia. Cancer Res 59 (23): 5989-94.
16. Rafty LA, Khachigian LM, (2001) Sp1 phosphorylation regulates inducible expression of platelet-derived growth factor B-chain gene via atypical protein kinase C-zeta. Nucleic Acids Res 29 (5): 1027-33.
17. Townsend KJ, Zhou P, Qian L, Bieszczad CK, Lowrey CH, et al. (1999) Regulation of MCL1 through a serum response factor/Elk-1-mediated mechanism links expression of a viability-promoting member of the BCL2 family to the induction of hematopoietic cell differentiation. J Biol Chem 274 (3): 1801-13.
18. Tang KF, Song GB, Shi YS, Yuan L, Li YH, (2010) Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1. Biochim Biophys Acta 1800 (3): 380-4.
19. Baron V, Adamson ED, Calogero A, Ragona G, Mercola D, (2006) The transcription factor Egr1 is a direct regulator of multiple tumor suppressors including TGFbeta1, PTEN, p53, and fibronectin. Cancer Gene Ther 13 (2): 115-24 Review.
20. Boone DN, Qi Y, Li Z, Hann SR, (2011) Egr1 mediates p53-independent c-Myc-induced apoptosis via a noncanonical ARF-dependent transcriptional mechanism. Proc Natl Acad Sci U S A 108 (2): 632-7.
21. Jeong SH, Park JH, Kim JN, Park YH, Shin SY, et al. (2010) Up-regulation of TNF-alpha secretion by cigarette smoke is mediated by Egr-1 in HaCaT human keratinocytes. Exp Dermatol 19 (8): e206-12.
22. Jin E, Liu J, Suehiro J, Yuan L, Okada Y, et al. (2009) Differential roles for ETS, CREB, and EGR binding sites in mediating VEGF receptor 1 expression in vivo. Blood 114 (27): 5557-66.
23. Lee KH, Kim JR, (2009) Hepatocyte growth factor induced up-regulations of VEGF through Egr-1 in hepatocellular carcinoma cells. Clin Exp Metastasis 26 (7): 685-92.
24. Figliola R, Busanello A, Vaccarello G, Maione R, (2008) Regulation of p57(KIP2) during muscle differentiation: role of Egr1, Sp1 and DNA hypomethylation. J Mol Biol 380 (2): 265-77.
25. Adamson ED, Mercola D, (2002) Egr1 transcription factor: multiple roles in prostate tumor cell growth and survival. Tumour Biol 23 (2): 93-102 Review.
26. Gitenay D, Baron VT, (2009) Is EGR1 a potential target for prostate cancer therapy? Future Oncol; 5 (7): 993-1003.
27. Boone DN, Qi Y, Li Z, Hann SR, (2011) Egr1 mediates p53-independent c-Myc-induced apoptosis via a non canonical ARF-dependent transcriptional mechanism. Proc Natl Acad Sci U S A 108 (2): 632-7.
28. Shareef MM, Cui N, Burikhanov R, Gupta S, Satishkumar S, et al. (2007) Role of tumor necrosis factor-alpha and TRAIL in high-dose radiation-induced bystander signaling in lung adenocarcinoma. Cancer Res 67 (24): 11811-20.
29. Cao X, Mahendran R, Guy GR, Tan YH, (1992) Protein phosphatase inhibitors induce the sustained expression of the Egr-1 gene and the hyperphosphorylation of its gene product. J Biol Chem 267: 12991-12997.
30. Bae MH, Jeong CH, Kim SH, Bae MK, Jeong JW, et al. (2002) Regulation of Egr-1 by association with the proteasome component C8. Biochim Biophys Acta 1592 (2): 163-7.
31. Yu J, de Belle I, Liang H, Adamson ED, (2004) Coactivating factors p300 and CBP are transcriptionally cross regulated by Egr1 in prostate cells, leading to divergent responses. Mol Cell 15 (1): 83-94.
32. Huang RP, Fan Y, deBelle I, Ni Z, Matheny W, Adamson ED, (1998) Egr-1 inhibits apoptosis during the UV response: correlation of cell survival with Egr-1 phosphorylation. Cell Death Differ 5 (1): 96-106.
33. PYu J, Zhang SS, Saito K, Williams S, Arimura Y, et al. (2009) PTEN regulation by Akt-EGR1-ARF-PTEN axis. EMBO J 28 (1): 21-33.
34. Hannoun Z, Greenhough S, Jaffray E, Hay RT, Hay DC, (2010) Post-translational modification by SUMO. Toxicology 278 (3): 288-93 Review.
35. Gareau JR, Lima CD, (2010) The SUMO pathway: emerging mechanisms that shape specificity, conjugation and recognition. Nat Rev Mol Cell Biol 11 (12): 861-71 Review.
36. Miteva M, Keusekotten K, Hofmann K, Praefcke GJK, Dohmen RJ, (2010) Sumoylation as a Signal for Polyubiquitylation and Proteasomal Degradation. Conjugation and Deconjugation of Ubiquitin Family Modifiers edited by Marcus Groettrup. Bookshelf ID NBK25447 Landes Bioscience and Springer Science+Business Media.
37. Cabodi S, Morello V, Masi A, Cicchi R, Broggio C, et al. (2009) Convergence of integrins and EGF receptor signaling via PI3K/Akt/FoxO pathway in early gene Egr-1 expression. J Cell Physiol 218 (2): 294-303.
38. Schreiber E, Matthias P, Müller MM, Schaffner W, (1989) Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells. Nucleic Acids Res 17 (15): 6419.
39. Moro L, Dolce L, Cabodi S, Bergatto E, Boeri Erba E, et al. (2002) Integrin-induced EGF receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines. J Biol Chem 277 (11): 9405-9414.
40. Chomczynski P, Sacchi N, (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem pp. 1 16256-9.
41. Liu T, Bohlken A, Kuljaca S, Lee M, Nguyen T, et al. (2005) The retinoid anticancer signal: mechanisms of target gene regulation. British Journal of Cancer 93: 310-318.
42. Groot M, Boxer LM, Thiel G, (2000) Nerve growth factor- and epidermal growth factor-regulated gene transcription in PC12 pheochromocytoma and INS-1 insulinoma cells. Eur J Cell Biol 79: 924-935.