Generating a knockdown transgene against Drosophila heterochromatic Tim17b gene encoding mitochondrial translocase subunit

PLoS ONE

Volumn 6, Issue 10, 2011, Pages

  Garabedian, Mikael ^a   Jarnik, Michael ^a   Kotova, Elena ^a   Tulin, Alexei V ^a  

^a FOX CHASE CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; INSECT PROTEIN; MITOCHONDRIAL PROTEIN; PROTEIN TIM17B; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG; DROSOPHILA PROTEIN; FLUORESCENT PROTEIN 583; HYBRID PROTEIN; PHOTOPROTEIN; TIM17B PROTEIN, DROSOPHILA;

ANIMAL TISSUE; APOPTOSIS; ARTICLE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; EXTRACELLULAR MATRIX; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GENE EXPRESSION REGULATION; GENE LOCATION; GENE SILENCING; GENETIC CODE; GENOME SIZE; HETEROCHROMATIN; INSECT CHROMOSOME; INSECT GENETICS; MITOCHONDRIAL MEMBRANE POTENTIAL; NONHUMAN; PROTEIN LOCALIZATION; PROTEIN STABILITY; PROTEIN TRANSPORT; REGULATORY MECHANISM; REPORTER GENE; RNA INTERFERENCE; TIM17B GENE; TRANSGENE; TRANSGENICS; ANIMAL; CYTOLOGY; GENETICS; HUMAN; MALE; METABOLISM; METHODOLOGY; MITOCHONDRION; PROTEIN SUBUNIT;

DROSOPHILA MELANOGASTER; EUKARYOTA;

ANIMALS; APOPTOSIS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; GENE KNOCKDOWN TECHNIQUES; HETEROCHROMATIN; HUMANS; LUMINESCENT PROTEINS; MALE; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIA; PROTEIN SUBUNITS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; RNA INTERFERENCE; TRANSGENES;

EID: 80053591072     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0025945     Document Type: Article

References (47)

1. Heitz E, (1933) Das geterochromatinder moose. J Jh Wiss 69: 762-818.
2. Blower MD, Sullivan B, Karpen GH, (2002) Conserved Organization of Centromeric Chromatin in Flies and Humans. Dev Cell 2: 319-330.
3. Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD et al, (2000) The Genome Sequence of Drosophila melanogaster. Science 287: 2185-2195.
4. Gatti M, Pimpinelli S, (1983) Cytological and genetical analysis of the Y chromosome of Drosophila melanogaster. Chromosoma 88: 593-617.
5. Lohe AR, Hilliker AJ, Roberts PA, (1993) Mapping Simple Repeated DNA Sequences in Heterochromatin of Drosophila melanogaster. Genetics 134: 1149-1174.
6. Nurminsky DI, Shevelyov YY, Nuzhdin SV, Gvozdev VA, (1994) Structure, molecular evolution and maintenance of copy number of extended repeated structures in the X-heterochromatin of Drosophila melanogaster. Chromosoma 103: 277-285.
7. Pimpinelli S, Berloco M, Fanti L, Dimitri P, Bonnacorsi S, et al. (1995) Transposable elements are stable structural components of Drosophila melanogaster heterochromatin. Genetics 92: 3804-3808.
8. Hilliker AJ, Appels R, Schalet A, (1980) The genetic analysis of D. melanogaster heterochromatin. Cell 21: 607-619.
9. Tulin AV, Kogan GL, Filipp D, Balakireva MD, Gvozdev VA, (1997) Heterochromatic Stellate gene cluster in Drosophila melanogaster: structure and molecular evolution. Genetics 146: 253-262.
10. Gatti M, Pimpinelli S, (1992) Functional Elements in Drosophila melanogaster Heterochromatin. Ann Rev Genet 26: 239-275.
11. Rossi F, Moschetti R, Caizzi R, Corradini N, Dimitri P, (2007) Cytogenic and Molecular Characterization of Heterochromatin Gene Models in Drosophila melanogaster. Genetics 175: 595-607.
12. Sinclair DAR, Schulze S, Silva E, Fitzpatrick KA, Honda BM, (2000) Essential genes in autosomal heterochromatin of Drosophila melanogaster. Genetics 109: 9-18.
13. Cryderman DE, Morris EJ, Biessmann H, Elgin SCR, Wallrath LL, (1999) Silencing at Drosophila telomeres: nuclear organization and chromatin structure play critical roles. EMBO J 18: 3724-3725.
14. Grewal SIS, Elgin SCR, (2002) Heterochromatin: new possibilities for the inheritance of structure. Curr Opin Genet Dev 12: 178-187.
15. Grewal SIS, Moazad D, (2003) Heterochromatin and Epigenetic Control of Gene Expression. Science 301: 798-802.
16. Tautz D, Hancock JM, Webb DA, Tautz C, Dover GA, (1988) Complete sequences of the rRNA genes of Drosophila melanogaster. Mol Biol Evol 5: 366-376.
17. Dimitri P, Caizzi R, Giordano E, Accardo MC, Lattanzi G, et al. (2009) Constitutive heterochromatin: a surprising variety of expressed sequences. Chromosoma 118: 419-435.
18. Tulin A, Stewart D, Spradling AC, (2002) The Drosophila heterochromatic gene encoding poly(ADP-ribose) polymerase (PARP) is required to modulate chromatin structure during development. Genes Dev 16: 2108-2119.
19. Grewal SIS, Jia S, (2007) Heterochromatin revisited. Nature Reviews Genetics 8: 35-46.
20. Ellermeier C, Higuchi EC, Phadnis N, Holm L, Geelhood JL, et al. (2010) RNAi and heterochromatin repress centromeric meiotic recombination. Proc Natl Acad Sci U S A 107: 8701-8705.
21. Rong YS, Titen SW, Xie HB, Golic MM, Bastiani M, et al. (2002) Targeted mutagenesis by homologous recombination in D. melanogaster. Genes Dev 16: 1568-1581.
22. Sondek J, Shortle D, (1992) A general strategy for random insertion and substitution mutagenesis: Substoichiometric coupling of trinucleotide phoshoramidites. Biochemistry 89: 3581-3585.
23. Cooley L, Kelley R, Spradling A, (1988) Insertional Mutagesis of the Drosophila Genome with Single P Elements. Science 239: 1121-1128.
24. Tavernarakis N, Wang SL, Dorovkov, M, Ryazanov A, Driscoll M, (2000) Heritable and inducible genetic interference by double-standed RNA encoded by transgenes. Nature Genetics 24: 180-183.
25. Fortier E, Belote JM, (2000) Temperature-Dependent Gene Silencing by an Expressed Inverted Repeat in Drosophila. Genesis 26: 240-244.
26. Baker MJ, Frazier AE, Gulbis JM, Ryan MT, (2007) Mitochondrial protein-import machinery: correlating structure with function. Trends in Cell Biology 17: 456-464.
27. Modica-Napalitano J, Singh KK, (2002) Mitochondria as targets for detection and treatment of cancer. Expert Reviews in Molecular Medicine 4: 1-19.
28. Peluso G, Nicolai R, Reda E, Benatti P, Barbarisi A, et al. (2000) Cancer and anticancer therapy-induced modifications on metabolism mediated by carnitine system. Journal of Cellular Physiology 182: 339-350.
29. Chang LO, Schnaitman CA, Morris HP, (1971) Comparison of the Mitochondrial Membrane Proteins in Rat Liver and Hepatomas. Cancer Research 31: 108-113.
30. Shigenaga MK, Hagen TM, Ames BN, (1994) Oxidative damage and mitochondrial decay in aging. Proc Natl Acad Sci U S A 91: 10771-10778.
31. Giordano E, Rendina R, Peluso I, Furia M, (2002) RNAi Triggered by Symmetrically Transcribed Transgenes in Drosophila melanogaster. Genetics 160: 637-648.
32. Cox RT, Spradling AC, (2003) A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis. Development 130: 1579-1590.
33. Clark IE, Dodson MW, Jiang C, Cao JH, Huh JR, et al. (2006) Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin. Nature 441: 1162-1166.
34. Westermann B, (2002) Merging mitochondria matters; Cellular role and molecular machinery of mitochondrial fusion. EMBO reports 3: 527-531.
35. Schulze S, Sinclair DAR, Silva E, Fitzpatrick, KA, Singh M, et al. (2001) Essential genes in proximal 3L heterochromatin of Drosophila melanogaster. Mol Gen 264: 782-789.
36. Coulthard AB, Alm C, Cealiac I, Sinclair DA, Honda BM, et al. (2010) Essential Loci in Centromeric Heterochromatin of Drosophila melanogaster. I: The Right Arm of Chromosome 2. Genetics 185: 479-495.
37. Irwin CC, Malkin LI, (1978) Differences in Total Mitochondrial Proteins and Proteins Synthesized by Mitochondria from Rat Liver and Morris Hepatomas. Cancer Research 38: 1584-1588.
38. Pedersen PL, (1978) Tumor mitochondria and the bioenergetics of cancer cells. Prog Exp Tumor Res 22: 190-274.
39. Weinhouse S, (1955) Oxidative metablism of neoplastic tissues. Adv Cancer Res 3: 269-325.
40. Carafoli E, (1980) Mitochondria and disease. Mol Aspects Med 3: 295-429.
41. FlyBase, (1999) The FlyBase database of the Drosophila genome projects and community literature. Nucl Acids Res 27: 85-88.
42. Manseau L, Baradaran A, Brower D, Budhu A, Elefant F, et al. (1997) GAL4 enhancer traps expressed in the embryo, larval brain, imaginal discs, and ovary of Drosophila. Dev Dyn 209: 310-322.
43. Spradling AC, Rubin GM, (1982) Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218: 341-347.
44. Prokhorova AV, Voloshina MA, Shostak NG, Barskii VE, Golubovskii MD, (1994) Preparation and primary genetic analysis of Drosophila melanogaster transformants line w9lz(b)/XXywf, containing mini-white genes, integrated in the genome during P-element-dependent transformation. Genetika 30: 874-878.
45. Kotova E, Jarnik M, Tulin AV, (2009) Poly (ADP-ribose) Polymerase 1 is required for protein localization to Cajal body. PLoS Genetics 5 (2): e1000387.
46. Phair RD, Misteli T, (2000) High mobility of proteins in mammalian cell nucleus. Nature 404: 604-609.
47. Pinnola A, Naumova N, Shah M, Tulin AV, (2007) Nucleosomal core histones mediate dynamic regulation of PARP1 protein binding to chromatin and induction of PARP1 enzymatic activity. J Biol Chem 282: 32511-32519.