Probing compressibility of the nuclear interior in wild-type and lamin deficient cells using microscopic imaging and computational modeling

Journal of Biomechanics

Volumn 44, Issue 15, 2011, Pages 2642-2648

  González Avalos, Paula ^a,b   Reichenzeller, Michaela ^b   Eils, Roland ^a,b   Gladilin, Evgeny ^b  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Cell nucleus; Compressibility; Mechanical phenotyping; Microscopic imaging; Nuclear lamina

Indexed keywords

CELL NUCLEUS; CELL TYPES; CELLULAR FORCE; CELLULAR PHENOTYPES; COMPUTATIONAL MODELING; FLUORESCENT CELLS; HIGH-THROUGHPUT MEASUREMENTS; IN-SITU; INTRACELLULAR STRUCTURES; MECHANICAL MODELING; MECHANICAL PHENOTYPING; MICRO MANIPULATION; MICROSCOPIC IMAGE; MICROSCOPIC IMAGING; NUCLEAR ENVELOPE; NUCLEAR LAMINA; NUMERICAL MODELS; OPTICAL METHODS; OVER-EXPRESSION; PHENOTYPING; POISSON'S RATIO; PROOF OF PRINCIPLES; STRUCTURAL PROTEINS; WILD TYPES; WILD-TYPE CELLS;

CELLS; COMPRESSIBILITY; CYTOLOGY; MICROSCOPIC EXAMINATION; TIME SERIES;

BIOMECHANICS;

CELL PROTEIN; LAMIN; LAMIN A; LAMIN C; STRUCTURAL PROTEIN;

ALGORITHM; ANIMAL CELL; ARTICLE; CELL COUNT; CELL KINETICS; CELL NUCLEUS MEMBRANE; CELL STRUCTURE; CELL SURFACE; CELL TYPE; COMPRESSION; CONTROLLED STUDY; CYTOLOGY; EMBRYO; IMAGE RECONSTRUCTION; MATHEMATICAL COMPUTING; MATHEMATICAL MODEL; MICROMANIPULATION; MICROSCOPE IMAGE; MICROSCOPY; MOLECULAR PROBE; MOUSE; NONHUMAN; NUCLEAR LAMINA; PHENOTYPIC VARIATION; PRIORITY JOURNAL; STRUCTURE ANALYSIS; WILD TYPE;

ANIMALS; CELLS, CULTURED; COMPRESSIVE STRENGTH; COMPUTER SIMULATION; ELASTICITY; FIBROBLASTS; IMAGE PROCESSING, COMPUTER-ASSISTED; LAMININ; MICE; MICE, KNOCKOUT; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; NUCLEAR ENVELOPE;

EID: 80053576368     PISSN: 00219290     EISSN: 18732380     Source Type: Journal    
DOI: 10.1016/j.jbiomech.2011.08.014     Document Type: Article

References (29)

1. Beaudouin J., et al. Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina. Cell 2002, 108(1):83-96.
2. Beskos D.E Boundary Element Methods in Mechanics 1987, North-Holland, Amsterdam.
3. - cells is caused by defective nucleo-cytoskeletal integrity: implications for the development of laminopathies. Human Molecular Genetics 2004, 13:2567-2580.
4. Caille N., Thoumine O., Tardy Y., Meister J.J. Contribution of the nucleus to the mechanical properties of endothelial cells. Journal of Biomechanics 2002, 35(2):177-187.
5. Ciarlet P.G. Mathematical Elasticity. vol. I: Three-Dimensional Elasticity, vol. 20 Studies in Mathematics and its Applications 1988, North-Holland, Amsterdam.
6. Dahl K.N., Engler A.J., Pa jerowski D., Discher D.E. Power-law rheology of isolated nuclei with deformation mapping of nuclear substructures. Biophysical Journal 2005, 89:2855-2864.
7. Dahl K.N., Booth-Gauthier E.A., Ladoux B. In the middle of it all: mutual mechanical regulation between the nucleus and the cytoskeleton. Journal of Biomechanics 2010, 43(1):2-8.
8. Gladilin E., et al. 3D fi{ligature}nite element analysis of uniaxial cell stretching: from image to insight. Physical Biology 2007, 4:104-113.
9. Gladilin E., et al. Shape normalization of 3D cell nuclei using elastic spherical mapping. Journal of Microscopy 2008, 231:105-114.
10. Gladilin E., et al. Contactless determination of nuclear compressibility by means of 3D image and model-based analysis of drug-induced cellular deformation. Journal of Microscopy 2010, 240(3):216-226.
11. Guck J., et al. The optical stretcher: a novel laser tool to micromanipulate cells. Biophysical Journal 2001, 81:767-784.
12. Guck J., et al. Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. Biophysical Journal 2005, 88(5):3689-3698.
13. Guilak F., Tedrow J.R., Burgkart R. Viscoelastic properties of the cell nucleus. Biochemical and Biophysical Research Communication 2000, 269:781-786.
14. Hutchison C.J., Worman H.J. A-type lamins: guardians of the soma?. Nature Cell Biology 2004, 6:1062-1067.
15. Iborra F.J., Cook P.R. The interdependence of nuclear structure and function. Current Opinion in Cell Biology 2002, 14:780-785.
16. Jean R.P., Chen C.S., Spector A.A. Finite-element analysis of the adhesion-cytoskeleton-nucleus mechanotransduction pathway during endothelial cell rounding: axisymmetric model. Journal of Biomechanical Engineering 2005, 127:594.
17. Kumar S., Weaver V.M. Mechanics, malignancy, and metastasis: the force journey of a tumor cell. Cancer Metastasis Reviews 2009, 28:113-127.
18. Lammerding J., et al. Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. Journal of Clinical Investigation 2004, 113:370-378.
19. Lammerding J., Dahl K.N., Discher D.E., Kamm R.D. Nuclear mechanics and methods. Methods in Cell Biology 2007, 83:269-294.
20. Leipzig N.D., Athanasiou K.A. Static compression of single chondrocytes catabolically modifies single-cell gene expression. Biophysical Journal 2008, 94(6):2412-2422.
21. Ofek G., Natoli R.M., Athanasiou K.A In situ mechanical properties of the chondrocyte cytoplasm and nucleus. Journal of biomechanics 2009, 42(7):873-877.
22. Pajerowski J.D., Dahl K.N., Zhong F.L., Sammak P.J., Discher D.E. Physical plasticity of the nucleus in stem cell differentiation. Proceedings of the National Academy of Sciences 2007, 104-40:15619-15624.
23. Rowat A., Lammerding J., Herrmann H., Aebi U. Towards an integrated understanding of the structure and mechanics of the cell nucleus. Bioessays 2008, 30:226-236.
24. Snapp E.L., Altan N., Lippincott-Schwartz J. Measuring Protein Mobility by Photobleaching GFP Chimeras in Living Cells. Current Protocols in Cell Biology 2001, John Wiley & Sons, Inc.
25. Sullivan T., et al. Loss of A-type lamin expression compromises nuclear envelope integrity leading to muscular dystrophy. Journal of Cell Biology 1999, 147:913-920.
26. Thoumine O., Ott A. Time scale dependent viscoelastic and contractile regimes in fibroblasts probed by microplate manipulation. Journal of Cell Science 1997, 110:2109-2116.
27. Vaziri A., Lee H., Mofrad M.R. Deformation of the cell nucleus under indentation: mechanics and mechanisms. Journal of Material Research 2006, 21:2126-2135.
28. Vaziri, A., Mofrad, M.R., 2007. Mechanics and deformation of the nucleus in micropipette aspiration experiment. Journal of Biomechanics.
29. Vaziri A., Gopinath A. Cell and biomolecular mechanics in silico. Nature Materials 2007, 7:15-23.