Optimized tandem amiRNA mediates stronger inhibitory effects on hepatitis B virus infection

Journal of Gastrointestinal and Liver Diseases

Volumn 20, Issue 3, 2011, Pages 271-278

  Pu, Chunwen ^a,b   Wang, Lifen ^b   Miao, Xiaohui ^c   Zhang, Yong ^a   Jiang, Chunmeng ^b   Liu, Wei ^a   Sun, Weixiang ^a   Gao, Qingwei ^a  

^a Dalian Sixth People's Hospital   (China)

^b DALIAN MEDICAL UNIVERSITY   (China)

^c SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

amiRNA; DNA; HBV; Multi; RNAi; Therapy

Indexed keywords

ANTIVIRUS AGENT; ARTIFICIAL MIRNA; HEPATITIS B SURFACE ANTIGEN; HEPATITIS B(E) ANTIGEN; MESSENGER RNA; MICRORNA; UNCLASSIFIED DRUG; VIRUS DNA;

ANTIVIRAL ACTIVITY; ARTICLE; CELL STRAIN HEPG2; CHEMOLUMINESCENCE; CONTROLLED STUDY; GENETIC TRANSFECTION; HEPATITIS B; HEPATITIS B VIRUS; IMMUNOASSAY; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; RNA SEQUENCE; VIRAL GENE DELIVERY SYSTEM; VIRUS INHIBITION; VIRUS STRAIN;

BASE SEQUENCE; DNA REPLICATION; DNA, VIRAL; GENETIC VECTORS; HEP G2 CELLS; HEPATITIS B; HEPATITIS B VIRUS; HUMANS; MICRORNAS; MOLECULAR SEQUENCE DATA; MUTATION; RNA INTERFERENCE; TRANSFECTION;

EID: 80053556995     PISSN: 18418724     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Kane M. Global programme for control of hepatitis B infection. Vaccine 1995; 13 Suppl 1: S47-49.
2. Ganem D, Prince AM. Hepatitis B virus infection--natural history and clinical consequences. N Engl J Med 2004; 350: 1118-1129.
3. Zoulim F. Antiviral therapy of chronic hepatitis B. Antiviral Res 2006; 71: 206-215.
4. Radhakrishnan SK, Layden TJ, Gartel AL. RNA interference as a new strategy against viral hepatitis. Virology 2004; 323: 173-181.
5. Wu HL, Huang LR, Huang CC, et al. RNA interference-mediated control of hepatitis B virus and emergence of resistant mutant. Gastroenterology 2005; 128: 708-716.
6. Chen Z, Xu ZF, Ye JJ, Yao HP, Zheng S, Ding JY. Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression. J Zhejiang Univ Sci B 2005; 6: 236-241.
7. Xia XG, Zhou H, Xu Z. Multiple shRNAs expressed by an inducible pol II promoter can knock down the expression of multiple target genes. Biotechniques 2006;41:64-68.
8. Liu YP, Haasnoot J, ter Brake O, Berkhout B, Konstantinova P. Inhibition of HIV-1 by multiple siRNAs expressed from a single microRNA polycistron. Nucleic Acids Res 2008;36:2811-2824.
9. Ely A, Naidoo T, Arbuthnot P. Effcient silencing of gene expression with modular trimeric Pol II expression cassettes comprising microRNA shuttles. Nucleic Acids Res 2009; 37: e91.
10. Dougherty JP, Temin HM. Determination of the rate of base-pair substitution and insertion mutations in retrovirus replication. J Virol 1988; 62: 2817-2822.
11. Leider JM, Palese P, Smith FI. Determination of the mutation rate of a retrovirus. J Virol 1988; 62: 3084-3091.
12. Gitlin L, Stone JK, Andino R. Poliovirus escape from RNA interference: short interfering RNA-target recognition and implications for therapeutic approaches. J Virol 2005; 79: 1027-1035.
13. Cheng TL, Chang WW, Su IJ, Lai MD, Huang W, Lei HY, Chang WT. Therapeutic inhibition of hepatitis B virus surface antigen expression by RNA interference. Biochem Biophys Res Commun 2005; 336: 820-883.
14. Wilson JA, Jayasena S, Khvorova A, et al. RNA interference blocks gene expression and RNA synthesis from hepatitis C replications propagated in human liver cells. Proc Natl Acad Sci U S A 2003;100: 2783-2788.
15. Starkey JL, Chiari EF, Isom HC. Hepatitis B virus (HBV)-specifc short hairpin RNA is capable of reducing the formation of HBV covalently closed circular (CCC) DNA, but has co effect on established CCC DNA in vitro. J Gen Virol 2009; 90: 115-126.
16. Ely A, Naidoo T, Mufamadi S, Crowther C, Arbuthnot P. Expressed anti-HBV primary microRNA shuttles inhibit viral replication effciently in vitro and in vivo. Mol Ther 2008; 16: 1105-1112.
17. Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ. A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc Natl Acad Sci U S A 2005; 102: 13212-13217.
18. Dickins RA, Hemann MT, Zilfou JT, et al. Probing tumor phenotypes using stable and regulated synthetic microRNA precursors. Nat Genet 2005; 37: 1289-1295.
19. Cai X, Hagedorn CH, Cullen BR. Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 2004; 10: 1957-1966.
20. Lee Y, Kim M, Han J, et al. MicroRNA genes are transcribed by RNA polymerase II. EMBO J 2004; 23: 4051-4060.
21. Giering JC, Grimm D, Storm TA, Kay MA. Expression of shRNA from a tissue-specifc pol II promoter is an effective and safe RNAi therapeutic. Mol Ther 2008; 16: 1630-1636.
22. Carmona S, Ely A, Crowther C, et al. Effective inhibition of HBV replication in vivo by anti-HBx short hairpin RNAs. Mol Ther 2006; 13:411-421.
23. Zhang T, Cheng T, Wei LH, et al. Construction of highly effective artifcial miRNA targeted to HIV-1 vif and the lentiviral-mediated antiviral research in vitro. Bing Du Xue Bao 2010;26:8-15.