Characterization of codon-optimized recombinant Candida rugosa Lipase 5 (LIP5)

Journal of Agricultural and Food Chemistry

Volumn 59, Issue 19, 2011, Pages 10693-10698

  Lee, Li Chiun ^a,f   Yen, Chih Chung ^a,c   Malmis, Conmar C ^a,d   Chen, Long Fang ^b   Chen, Jen Chieh ^a   Lee, Guan Chiun ^e   Shaw, Jei Fu ^a,f  

^a AGRICULTURAL BIOTECHNOLOGY RESEARCH CENTER   (Taiwan)

^b INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

^c NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^d MINDANAO STATE UNIVERSITY ILIGAN INSTITUTE OF TECHNOLOGY   (Philippines)

^e NATIONAL TAIWAN NORMAL UNIVERSITY   (Taiwan)

^f NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Candida rugosa lipase 5 (LIP5); hydrolysis; specificity; substrate

Indexed keywords

BUTYRYL-COA; CANDIDA RUGOSA LIPASE; CHOLESTERYL LAURATE; CODON OPTIMIZATION; COMMERCIAL LIPASE; ISOFORMS; PH-DEPENDENT; SPECIFICITY; SUBSTRATE SPECIFICITY; TRIACYLGLYCEROLS;

AMINO ACIDS; CANDIDA; CATALYST ACTIVITY; CHARACTERIZATION; ESTERS; GLYCEROL; HYDROLYSIS; OPTIMIZATION; VOLATILE FATTY ACIDS; YEAST;

SUBSTRATES;

4-NITROPHENYL BUTYRATE; BUTYRIC ACID 4 NITROPHENYL ESTER; BUTYRIC ACID DERIVATIVE; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; CANDIDA; CODON; COMPARATIVE STUDY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HYDROLYSIS; METABOLISM; MUTAGENESIS;

BUTYRATES; CANDIDA; CODON; HYDROLYSIS; LIPASE; MUTAGENESIS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

CANDIDA RUGOSA;

EID: 80053503843     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf202161a     Document Type: Article

References (22)

1. Linfield, W. M.; Obrien, D. J.; Serota, S.; Barauskas, R. A. Lipid-lipase interactions. 1. Fat splitting with lipase from Candida rugosa J. Am. Oil Chem. Soc. 1984, 61, 1067-1071 (Pubitemid 15428180)
2. Valero, F.; Ayats, F.; Lopezsantin, J.; Poch, M. Lipase production by Candida rugosa-fermentation behavior Biotechnol. Lett. 1988, 10, 741-744
3. Valero, F.; Poch, M.; Sola, C.; Lapa, S.; Lima, C. Online monitoring of lipase production in fermentation processes Biotechnol. Tech. 1991, 5, 251-254
4. Longhi, S.; Fusetti, F.; Grandori, R.; Lotti, M.; Vanoni, M.; Alberghina, L. Coning and nucleotide sequences of 2 lipase genes from Candida cylindracea Biochim. Biophys. Acta 1992, 1131, 227-232
5. Lotti, M.; Grandori, R.; Fusetti, F.; Longhi, S.; Brocca, S.; Tramontano, A.; Alberghina, L. Cloning and analysis of Candida cylindracea lipase sequences Gene 1993, 124, 45-55 (Pubitemid 23065966)
6. Lee, G. C.; Tang, S. J.; Sun, K. H.; Shaw, J. F. Analysis of the gene family encoding lipases in Candida rugosa by competitive reverse transcription-PCR Appl. Environ. Microbiol. 1999, 65, 3888-3895
7. Chang, S. W.; Shieh, C. J.; Lee, G. C.; Shaw, J. F. Multiple mutagenesis of the Candida rugosa LIP1 gene and optimum production of recombinant LIP1 expressed in Pichia pastoris Appl. Microbiol. Biotechnol. 2005, 67, 215-224 (Pubitemid 40591273)
8. Chang, S. W.; Lee, G. C.; Shaw, J. F. Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase J. Agric. Food Chem. 2006, 54, 815-822
9. Srivastava, A.; Akoh, C. C.; Chang, S. W.; Lee, G. C.; Shaw, J. F. Candida rugosa lipase LIP1-catalyzed transesterification to produce human milk fat substitute J. Agric. Food Chem. 2006, 54, 5175-5181 (Pubitemid 44140918)
10. Nam, K. H.; Lee, W. H.; Rhee, K. H.; Hwang, K. Y. Structural characterization of the bifunctional glucanase-Xylanase CelM2 reveals the metal effect and substrate-binding moiety Biochem. Biophys. Res. Commun. 2010, 391, 1726-1730
11. Lee, L. C.; Chen, Y. T.; Yen, C. C.; Chiang, T. C. Y.; Tang, S. J.; Lee, G. C.; Shaw, J. F. Altering the substrate specificity of Candida rugosa LIP4 by engineering the substrate-binding sites J. Agric. Food Chem. 2007, 55, 5103-5108
12. Chang, S. W.; Lee, G. C.; Shaw, J. F. Efficient production of active recombinant Candida rugosa LIP3 lipase in Pichia pastoris and biochemical characterization of the purified enzyme J. Agric. Food Chem. 2006, 54, 5831-5838
13. Yen, C. C.; Malmis, C. C.; Lee, G. C.; Lee, L. C.; Shaw, J. F. Site-Specific Saturation Mutagenesis on Residues 132 and 450 of Candida rugosa LIP2 Enhances Catalytic Efficiency and Alters Substrate Specificity in Various Chain Lengths of Triglycerides and Esters J. Agric. Food Chem. 2010, 58, 10899-10905
14. Lotti, M.; Monticelli, S.; Montesinos, J. L.; Brocca, S.; Valero, F.; Lafuente, J. Physiological control on the expression and secretion of Candida rugosa lipase Chem. Phys. Lipids 1998, 93, 143-148 (Pubitemid 28383213)
15. Hsu, K. H.; Lee, G. C.; Shaw, J. F. Promoter analysis and differential expression of the Candida rugosa lipase gene family in response to culture conditions J. Agric. Food Chem. 2008, 56, 1992-1998
16. Akoh, C. C.; Lee, G. C.; Shaw, J. F. Protein engineering and applications of Candida rugosa lipase isoforms Lipids 2004, 39, 513-526 (Pubitemid 39372347)
17. Lee, G. C.; Lee, L. C.; Sava, V.; Shaw, J. F. Multiple mutagenesis of non-universal serine codons of the Candida rugosa LIP2 gene and biochemical characterization of purified recombinant LIP2 lipase overexpressed in Pichia pastoris Biochem. J. 2002, 366, 603-611 (Pubitemid 35001705)
18. Zavodszky, P.; Kardos, J.; Svingor, A.; Petsko, G. A. Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 7406-7411 (Pubitemid 28293265)
19. Sakaguchi, M.; Matsuzaki, M.; Niimiya, K.; Seino, J.; Sugahara, Y.; Kawakita, M. Role of proline residues in conferring thermostability on aqualysin I J. Biochem. 2007, 141, 213-220 (Pubitemid 46775758)
20. Lee, L. C.; Liaw, Y. C.; Lee, Y. L.; Shaw, J. F. Enhanced preference for pi-bond containing substrates is correlated to Pro110 in the substrate-binding tunnel of Escherichia coli thioesterase I/protease I/lysophospholipase L-1 Biochim. Biophys. Acta 2007, 1774, 959-967
21. Fojan, P.; Jonson, P. H.; Petersen, M. T. N.; Petersen, S. B. What distinguishes an esterase from a lipase: A novel structural approach Biochimie 2000, 82, 1033-1041
22. Kirk, O.; Borchert, T. V.; Fuglsang, C. C. Industrial enzyme applications Curr. Opin. Biotechnol. 2002, 13, 345-351 (Pubitemid 35254093)