Transketolase from Escherichia coli: A practical procedure for using the biocatalyst for asymmetric carbon-carbon bond synthesis

Tetrahedron Asymmetry

Volumn 7, Issue 8, 1996, Pages 2185-2188

  Morris, K Gail ^a   Smith, Mark E B ^a   Turner, Nicholas J ^a   Lilly, Malcolm D ^b   Mitra, Robin K ^b   Woodley, John M ^b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PYRUVIC ACID DERIVATIVE; TRANSKETOLASE;

ARTICLE; DRUG SYNTHESIS; ENZYME ACTIVITY; ESCHERICHIA COLI; PRIORITY JOURNAL;

EID: 0030220903     PISSN: 09574166     EISSN: None     Source Type: Journal    
DOI: 10.1016/0957-4166(96)00266-2     Document Type: Article

References (19)

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15. 9. HPA assayed by the reported method; A.W. Holldorf, in Methods in Enzymology, Academic Press, New York, 1966, Volume 3, p.578.
16. 3) was added to aid solubility of the remaining aldehyde and hence ensure complete reaction. The reaction was terminated after 24h by which time uptake of aq. HCl had ceased. Addition of flash silica (̃4g) followed by removal of the water/THF under reduced pressure and flash chromatography (ethyl acetate-petrol = 2:3 then ethyl acetate-methanol = 9:1) afforded 5-O-benzyl-D-xylulose 3a (1.262 g, 5.3 mmol, 76%).
17. 11. We are currently utilising the triol 3a for the synthesis of target natural products, details of which will be published in due course.
18. 12. F. Dickens, Biochemical Preparations , 1962, 9, 86.
19. 3) followed by careful addition of aq, KOH (5M) until a stable pH of 9.0 was achieved. At no time during the addition was the pH allowed to exceed 9.5. The reaction mixture was then adjusted to pH 5.0 by addition of glacial acetic acid and concentrated under vacuum until crystals of the product had just begun to appear. Subsequent refrigeration overnight, filtration and drying over phosphorus pentoxide afforded highly crystalline potassium hydroxypyruvate (24.46g, 0.17 mol, 36%).