High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells

PLoS ONE

Volumn 6, Issue 9, 2011, Pages

  Gutierrez, Edgar ^a   Tkachenko, Eugene ^a,b   Besser, Achim ^c   Sundd, Prithu ^d   Ley, Klaus ^d   Danuser, Gaudenz ^c   Ginsberg, Mark H ^b   Groisman, Alex ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

^d LA JOLLA INSTITUTE FOR ALLERGY AND IMMUNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SILICONE GEL;

ADHERENT CELL; ARTICLE; BIOMECHANICS; CONTROLLED STUDY; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; MICROFLUIDICS; MICROSCOPY; REFRACTION INDEX; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; TRACTION FORCE MICROSCOPY; YOUNG MODULUS; CELL ADHESION; CELL CULTURE; CELL MOTION; CHEMISTRY; CYTOLOGY; FLUORESCENCE MICROSCOPY; METABOLISM; METHODOLOGY; PHYSIOLOGY; REFRACTOMETRY; UMBILICAL VEIN ENDOTHELIAL CELL;

CELL ADHESION; CELL MOVEMENT; CELLS, CULTURED; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; MICROSCOPY; MICROSCOPY, FLUORESCENCE; REFRACTOMETRY; SILICONE GELS;

EID: 80053078037     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0023807     Document Type: Article

References (33)

1. Nemir S, West JL, (2009) Synthetic materials in the study of cell response to substrate rigidity. Ann Biomed Eng 38: 2-20.
2. Brown AE, Discher DE, (2009) Conformational changes and signaling in cell and matrix physics. Curr Biol 19: R781-789.
3. Paszek MJ, Zahir N, Johnson KR, Lakins JN, Rozenberg GI, et al. (2005) Tensional homeostasis and the malignant phenotype. Cancer Cell 8: 241-254.
4. Ingber DE, Folkman J, (1989) Mechanochemical switching between growth and differentiation during fibroblast growth factor-stimulated angiogenesis in vitro: role of extracellular matrix. J Cell Biol 109: 317-330.
5. Reilly GC, Engler AJ, (2010) Intrinsic extracellular matrix properties regulate stem cell differentiation. J Biomech 43: 55-62.
6. Engler AJ, Sen S, Sweeney HL, Discher DE, (2006) Matrix elasticity directs stem cell lineage specification. Cell 126: 677-689.
7. Discher DE, Mooney DJ, Zandstra PW, (2009) Growth factors, matrices, and forces combine and control stem cells. Science 324: 1673-1677.
8. Zaman MH, Trapani LM, Sieminski AL, Mackellar D, Gong H, et al. (2006) Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis. Proc Natl Acad Sci U S A 103: 10889-10894.
9. Krndija D, Schmid H, Eismann JL, Lother U, Adler G, et al. (2010) Substrate stiffness and the receptor-type tyrosine-protein phosphatase alpha regulate spreading of colon cancer cells through cytoskeletal contractility. Oncogene 29: 2724-2738.
10. Isenberg BC, Dimilla PA, Walker M, Kim S, Wong JY, (2009) Vascular smooth muscle cell durotaxis depends on substrate stiffness gradient strength. Biophys J 97: 1313-1322.
11. Dubin-Thaler BJ, Hofman JM, Cai Y, Xenias H, Spielman I, et al. (2008) Quantification of cell edge velocities and traction forces reveals distinct motility modules during cell spreading. PLoS One 3: e3735.
12. Guo WH, Frey MT, Burnham NA, Wang YL, (2006) Substrate rigidity regulates the formation and maintenance of tissues. Biophys J 90: 2213-2220.
13. Sabass B, Gardel ML, Waterman CM, Schwarz US, (2008) High resolution traction force microscopy based on experimental and computational advances. Biophys J 94: 207-220.
14. Axelrod D, (2001) Total internal reflection fluorescence microscopy in cell biology. Traffic 2: 764-774.
15. Hu K, Ji L, Applegate KT, Danuser G, Waterman-Storer CM, (2007) Differential transmission of actin motion within focal adhesions. Science 315: 111-115.
16. Pelkmans L, Zerial M, (2005) Kinase-regulated quantal assemblies and kiss-and-run recycling of caveolae. Nature 436: 128-133.
17. Reichert WM, Truskey GA, (1990) Total Internal-Reflection Fluorescence (Tirf) Microscopy.1. Modeling Cell Contact Region Fluorescence. Journal Of Cell Science 96: 219-230.
18. Sundd P, Gutierrez E, Pospieszalska MK, Zhang H, Groisman A, et al. (2010) Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling. Nature Meth 7: 821-824.
19. Pelham RJ, Wang YL, (1997) Cell locomotion and focal adhesions are regulated by substrate flexibility. Proceedings Of The National Academy Of Sciences Of The United States Of America 94: 13661-13665.
20. Harris AK, Stopak D, Wild P, (1981) Fibroblast Traction As A Mechanism For Collagen Morphogenesis. Nature 290: 249-251.
21. Burton K, Taylor DL, (1997) Traction forces of cytokinesis measured with optically modified elastic substrata. Nature 385: 450-454.
22. Balaban NQ, Schwarz US, Riveline D, Goichberg P, Tzur G, et al. (2001) Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates. Nat Cell Biol 3: 466-472.
23. Schwarz US, Balaban NQ, Riveline D, Bershadsky A, Geiger B, et al. (2002) Calculation of forces at focal adhesions from elastic substrate data: The effect of localized force and the need for regularization. Biophysical Journal 83: 1380-1394.
24. Iwadate Y, Yumura S, (2008) Actin-based propulsive forces and myosin-II-based contractile forces in migrating Dictyostelium cells. Journal Of Cell Science 121: 1314-1324.
25. Iwadate Y, Yumura S, (2008) Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopies. Biotechniques 44: 739-+.
26. Gutierrez E, Groisman A, (2011) Measurements of elastic moduli of silicone gel substrates with a microfluidic device. Submitted for publication.
27. Mark JE, (1999) Polymer Data Handbook. Oxford Oxford University Press.
28. Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, et al. (2008) Lifeact: a versatile marker to visualize F-actin. Nat Methods 5: 605-607.
29. Feral CC, Zijlstra A, Tkachenko E, Prager G, Gardel ML, et al. (2007) CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling. J Cell Biol 178: 701-711.
30. Ji L, Danuser G, (2005) Tracking quasi-stationary flow of weak fluorescent signals by adaptive multi-frame correlation. Journal Of Microscopy-Oxford 220: 150-167.
31. Dembo M, Wang YL, (1999) Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophysical Journal 76: 2307-2316.
32. Stricker J, Sabass B, Schwarz US, Gardel ML, (2010) Optimization of traction force microscopy for micron-sized focal adhesions. Journal Of Physics-Condensed Matter 22.
33. Saez A, Buguin A, Silberzan P, Ladoux B, (2005) Is the mechanical activity of epithelial cells controlled by deformations or forces? Biophys J 89: L52-54.