Phytosterol supplementation reduces metabolic activity and slows cell growth in cultured rat cardiomyocytes

British Journal of Nutrition

Volumn 106, Issue 4, 2011, Pages 540-548

  Danesi, Francesca ^a   Ferioli, Federico ^a   Fiorenza Caboni, Maria ^a   Boschetti, Elisa ^b   Di Nunzio, Mattia ^b   Verardo, Vito ^a   Valli, Veronica ^a   Astolfi, Annalisa ^b   Pession, Andrea ^c   Bordoni, Alessandra ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Cardiomyocytes; Cytotoxicity; Metabolic activity; Phytosterols

Indexed keywords

AVENASTEROL; BRASSICASTEROL; CAMPESTANOL; CAMPESTEROL; CHOLESTEROL; CLEROSTEROL; FUCOSTEROL; LACTATE DEHYDROGENASE; PHYTOSTEROL; SITOSTANOL; SITOSTEROL; STIGMASTEROL; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CYCLE; CELL GROWTH; CELL MEMBRANE; CELL METABOLISM; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DIET SUPPLEMENTATION; ENZYME RELEASE; HEART MUSCLE CELL; MITOCHONDRION; NEWBORN; NONHUMAN; RAT;

ANIMALS; ANIMALS, NEWBORN; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; CELLS, CULTURED; CHOLESTEROL; HEART VENTRICLES; MYOCYTES, CARDIAC; OSMOLAR CONCENTRATION; PHYTOSTEROLS; RATS; RATS, WISTAR;

RATTUS;

EID: 80053006898     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1017/S0007114511000626     Document Type: Article

References (42)

1. Bradford PG & Awad AB (2007) Phytosterols as anticancer compounds. Mol Nutr Food Res 51, 161-170. (Pubitemid 47083247)
2. Abumweis SS, Barake R & Jones PJ (2008) Plant sterols/stanols as cholesterol lowering agents: a meta-analysis of randomized controlled trials. Food Nutr Res 52 (Epublication ahead of print version 18 August 2008).
3. Rozner S & Garti N (2006) The activity and absorption relationship of cholesterol and phytosterols. Colloids Surf A: Physicochem Eng Asp 282-283, 435-456. (Pubitemid 43816829)
4. Awad AB, Chen YC, Fink CS, et al. (1996) Beta-sitosterol inhibits HT-29 human colon cancer cell growth and alters membrane lipids. Anticancer Res 16, 2797-2804. (Pubitemid 26388254)
5. Tapiero H, Townsend DM & Tew KD (2003) Phytosterols in the prevention of human pathologies. Biomed Pharmacother 57, 321-325. (Pubitemid 37237598)
6. Awad AB, Smith AJ & Fink CS (2001) Plant sterols regulate rat vascular smooth muscle cell growth and prostacyclin release in culture. Prostaglandins Leukot Essent Fatty Acids 64, 323-330. (Pubitemid 32695305)
7. Windler E, Zyriax BC, Kuipers F, et al. (2009) Association of plasma phytosterol concentrations with incident coronary heart disease Data from the CORA study, a case-control study of coronary artery disease in women. Atherosclerosis 203, 284-290.
8. Ju YH, Clausen LM, Allred KF, et al. (2004) β-sitosterol, β-sitosterol glucoside, and a mixture of β-sitosterol and β-sitosterol glucoside modulate the growth of estrogen-responsive breast cancer cells in vitro and in ovariectomized athymic mice. J Nutr 134, 1145-1151. (Pubitemid 38619954)
9. Jones PJ & AbuMweis SS (2009) Phytosterols as functional food ingredients: linkages to cardiovascular disease and cancer. Curr Opin Clin Nutr Metab Care 12, 147-151.
10. Rubis B, Paszel A, Kaczmarek M, et al. (2008) Beneficial or harmful influence of phytosterols on human cells? Br J Nutr 100, 1183-1191.
11. Mayr M & Xu Q (2001) Smooth muscle cell apoptosis in arteriosclerosis. Exp Gerontol 36, 969-987. (Pubitemid 32515215)
12. Kim JC, Kang BH, Shin CC, et al. (2002) Subchronic toxicity of plant sterol esters administered by gavage to Sprague-Dawley rats. Food Chem Toxicol 40, 1569-1580.
13. Marangoni F & Poli A (2010) Phytosterols and cardiovascular health. Pharmacol Res 61, 193-199.
14. Vanstone CA, Raeini-Sarjaz M, Parsons WE, et al. (2002) Unesterified plant sterols and stanols lower LDL-cholesterol concentrations equivalently in hypercholesterolemic persons. Am J Clin Nutr 76, 1272-1278. (Pubitemid 35416562)
15. Hovenkamp E, Demonty I, Plat J, et al. (2008) Biological effects of oxidized phytosterols: a review of the current knowledge. Prog Lipid Res 47, 37-49. (Pubitemid 350216621)
16. Sweeley CC, Bentley R, Makita M, et al. (2002) Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. J Am Chem Soc 85, 2497-2507.
17. Pelillo M, Iafelice G, Marconi E, et al. (2003) Identification of plant sterols in hexaploid and tetraploid wheats using gas chromatography with mass spectrometry. Rapid Commun Mass Spectrom 17, 2245-2252. (Pubitemid 37243306)
18. Rose-Sallin C, Huggett AC, Bosset JO, et al. (1995) Quantification of cholesterol oxidation products in milk powders using [2H7]cholesterol to monitor cholesterol autoxidation artifacts. J Agric Food Chem 43, 935-941.
19. Dutta PC (2002) Determination of phytosterol oxidation products in foods and biological samples. In Cholesterol and Phytosterol Oxidation Products-Analysis, Occurrence, and Biological Effects, pp. 335-374 [F Guardiola, PC Dutta, R Codony and GP Savage, editors]. Champaign, IL: AOCS Press.
20. Danesi F, Di Nunzio M, Boschetti E, et al. (2009) Green tea extract selectively activates peroxisome proliferator-activated receptor beta/delta in cultured cardiomyocytes. Br J Nutr 101, 1736-1739.
21. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254.
22. Ahmad S, White CW, Chang LY, et al. (2001) Glutamine protects mitochondrial structure and function in oxygen toxicity. Am J Physiol Lung Cell Mol Physiol 280, L779-L791.
23. Sander BD, Addis PB, Park SW, et al. (1989) Quantification of cholesterol oxidation products in a variety of foods. J Food Prot 52, 109-114.
24. Wichmann G, Daegelmann C, Herbarth O, et al. (2004) Inflammatory activity in river-water samples. Environ Toxicol 19, 594-602. (Pubitemid 39552269)
25. Nicoletti I, Migliorati G, Pagliacci MC, et al. (1991) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 139, 271-279.
26. Korzeniewski C & Callewaert DM (1983) An enzyme-release assay for natural cytotoxicity. J Immunol Methods 64, 313-320. (Pubitemid 14243095)
27. Awad AB, Downie AC & Fink CS (2000) Inhibition of growth and stimulation of apoptosis by beta-sitosterol treatment of MDA-MB-231 human breast cancer cells in culture. Int J Mol Med 5, 541-545.
28. Awad AB, Roy R & Fink CS (2003) Beta-sitosterol, a plant sterol, induces apoptosis and activates key caspases in MDA-MB-231 human breast cancer cells. Oncol Rep 10, 497-500.
29. Bao L, Li Y, Deng SX, et al. (2006) Sitosterol-containing lipoproteins trigger free sterol-induced caspase-independent death in ACAT-competent macrophages. J Biol Chem 281, 33635-33649. (Pubitemid 46036743)
30. Gomez LA, Alekseev AE, Aleksandrova LA, et al. (1997) Use of the MTT assay in adult ventricular cardiomyocytes to assess viability: effects of adenosine and potassium on cellular survival. J Mol Cell Cardiol 29, 1255-1266. (Pubitemid 27211906)
31. Opie LH (1998) Fuels: aerobic and anaerobic metabolism. In The Heart: Physiology, from Cell to Circulation, 3rd ed., pp. 295-342 [RWSK Weinberg, editor]. Philadelphia, PA: Lippincott-Raven.
32. Kakinuma Y, Miyauchi T, Yuki K, et al. (2000) Mitochondrial dysfunction of cardiomyocytes causing impairment of cellular energy metabolism induces apoptosis, and concomitant increase in cardiac endothelin-1 expression. J Cardiovasc Pharmacol 36, S201-S204.
33. Taegtmeyer H (2010) Tracing cardiac metabolism in vivo: one substrate at a time. J Nucl Med 51, 80S-87S.
34. Chang TY, Chang CC & Cheng D (1997) Acyl-coenzyme A:cholesterol acyltransferase. Annu Rev Biochem 66, 613-638.
35. Tabas I (2002) Consequences of cellular cholesterol accumulation: basic concepts and physiological implications. J Clin Invest 110, 905-911.
36. Temel RE, Gebre AK, Parks JS, et al. (2003) Compared with Acyl-CoA:cholesterol O-acyltransferase (ACAT) 1 and lecithin:cholesterol acyltransferase, ACAT2 displays the greatest capacity to differentiate cholesterol from sitosterol. J Biol Chem 278, 47594-47601. (Pubitemid 37523203)
37. Liu J, Chang CC, Westover EJ, et al. (2005) Investigating the allosterism of acyl-CoA:cholesterol acyltransferase (ACAT) by using various sterols: in vitro and intact cell studies. Biochem J 391, 389-397. (Pubitemid 41532437)
38. Melchert RB, Liu H, Granberry MC, et al. (2001) Lovastatin inhibits phenylephrine-induced ERK activation and growth of cardiac. Cardiovasc Toxicol 1, 237-252. (Pubitemid 37013751)
39. Vemuri R & Philipson KD (1989) Influence of sterols and phospholipids on sarcolemmal and sarcoplasmic reticular cation transporters. J Biol Chem 264, 8680-8685. (Pubitemid 19151586)
40. Naito Y, Nagata T, Takano Y, et al. (2003) Rapeseed oil ingestion and exacerbation of hypertension-related conditions in stroke prone spontaneously hypertensive rats. Toxicology 187, 205-216. (Pubitemid 36423313)
41. Chien MH, Lee TS, Liang YC, et al. (2010) Beta-sitosterol inhibits cell cycle progression of rat aortic smooth muscle cells through increases of p21cip1 protein. J Agric Food Chem 58, 10064-10069.
42. Lizard G (2008) Phytosterols: to be or not to be toxic; that is the question. Br J Nutr 100, 1150-1151.