Unexpected stress-Reducing effect of PhaP, a poly(3-hydroxybutyrate) granule-associated protein, in Escherichia coli

Applied and Environmental Microbiology

Volumn 77, Issue 18, 2011, Pages 6622-6629

  Almeida, de Alejandra ^a,b   Catone, Mariela V ^a   Rhodius, Virgil A ^b   Gross, Carol A ^b,c   Pettinari, M Julia ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COEXPRESSED; E. COLI; GRANULE-ASSOCIATED PROTEINS; HEAT STRESS; HEAT-SHOCK; HETEROLOGOUS PROTEINS; MISFOLDED PROTEINS; PCR ANALYSIS; PHB ACCUMULATION; POLY(3-HYDROXYBUTYRATE); POLYMER PRODUCTION; PROTECTIVE EFFECTS; PROTEIN LEVEL; RECOMBINANT ESCHERICHIA COLI; REVERSE TRANSCRIPTION; STRESS CONDITION; STRESS-RELATED GENE; SUPEROXIDES;

BIODEGRADABLE POLYMERS; ESCHERICHIA COLI; GENES; GRANULATION; HEAT RESISTANCE; OXYGEN; PROTEINS; STRAIN; TRANSCRIPTION;

BIOSYNTHESIS;

BACTERIAL PROTEIN; CHAPERONE; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; HYDROXYBUTYRIC ACID; PHAP PROTEIN, BACTERIA; POLY(3 HYDROXYBUTYRIC ACID); POLY-BETA-HYDROXYBUTYRATE; POLYESTER; RECOMBINANT PROTEIN;

BIODEGRADATION; COLIFORM BACTERIUM; EXPERIMENTAL STUDY; POLYMER; POLYMERASE CHAIN REACTION; PROTEIN; REAL TIME; RECOMBINATION; TEMPERATURE TOLERANCE;

ARTICLE; AZOTOBACTER; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION PROFILING; GENETICS; METABOLISM; PHYSIOLOGICAL STRESS; PHYSIOLOGY;

AZOTOBACTER; BACTERIAL PROTEINS; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION PROFILING; HYDROXYBUTYRATES; MOLECULAR CHAPERONES; POLYESTERS; RECOMBINANT PROTEINS; STRESS, PHYSIOLOGICAL;

AZOTOBACTER; ESCHERICHIA COLI;

EID: 80052815932     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05469-11     Document Type: Article

References (45)

1. Allen, S. P., J. O. Polazzi, J. K. Gierse, and A. M. Easton. 1992. Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in Escherichia coli. J. Bacteriol. 174:6938-6947.
2. Almirón, M., A. J. Link, D. Furlong, and R. Kolter. 1992. A novel DNAbinding protein with regulatory and protective roles in starved Escherichia coli. Genes Dev. 6:2646-2654.
3. Bianchi, A. A., and F. Baneyx. 1999. Hyperosmotic shock induces the sigma32 and sigmaE stress regulons of Escherichia coli. Mol. Microbiol. 34:1029-1038.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Braunegg, G., B. Sonnleitner, and R. M. Lafferty. 1978. A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in bacterial biomass. Eur. J. Appl. Microbiol. Biotechnol. 6:29-37.
6. Casadaban, M. J. 1976. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol. 104:541-555.
7. Chang, Y. Y., A. Y. Wang, and J. E. Cronan, Jr. 1994. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol. Microbiol. 11:1019-1028.
8. Clomburg, J. M., and R. Gonzalez. 2010. Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology. Appl. Microbiol. Biotechnol. 86:419-434.
9. de Almeida, A., P. I. Nikel, A. M. Giordano, and M. J. Pettinari. 2007. Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli. Appl. Environ. Microbiol. 73:7912-7916.
10. Gill, R. T., J. J. Valdes, and W. E. Bentley. 2000. A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli. Metab. Eng. 2:178-189.
11. Gonzalez, R., et al. 2003. Gene array-based identification of changes that contribute to ethanol tolerance in ethanologenic Escherichia coli: comparison of KO11 (parent) to LY01 (resistant mutant). Biotechnol. Prog. 19:612-623.
12. Gross, C. A. 1996. Function and regulation of the heat shock proteins. Escherichia coli and Salmonella, p. 1382-1399. ASM Press, Washington DC.
13. Guisbert, E., T. Yura, V. A. Rhodius, and C. A. Gross. 2008. Convergence of molecular, modeling, and systems approaches for an understanding of the Escherichia coli heat shock response. Microbiol. Mol. Biol. Rev. 72:545-554.
14. Han, M. J., et al. 2006. Analysis of poly(3-hydroxybutyrate) granule-associated proteome in recombinant Escherichia coli. J. Microbiol. Biotechnol. 16:901-910.
15. Han, M. J., S. J. Park, T. J. Park, and S. Y. Lee. 2004. Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli. Biotechnol. Bioeng. 88:426-436.
16. Han, M. J., S. S. Yoon, and S. Y. Lee. 2001. Proteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate). J. Bacteriol. 183:301-308.
17. Hengge-Aronis, R. 2002. Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase. Microbiol. Mol. Biol. Rev. 66:373-395.
18. Hoffmann, F., and U. Rinas. 2004. Stress induced by recombinant protein production in Escherichia coli. Adv. Biochem. Eng. Biotechnol. 89:73-92.
19. Jürgen, B., et al. 2000. Monitoring of genes that respond to overproduction of an insoluble recombinant protein in Escherichia coli glucose-limited fedbatch fermentations. Biotechnol. Bioeng. 70:217-224.
20. Kabir, M. M., and K. Shimizu. 2003. Fermentation characteristics and protein expression patterns in a recombinant Escherichia coli mutant lacking phosphoglucose isomerase for poly(3-hydroxybutyrate) production. Appl. Microbiol. Biotechnol. 62:244-255.
21. Khanna, S., and A. K. Srivastava. 2005. Recent advances in microbial polyhydroxyalkanoates. Process. Biochem. 40:607-619.
22. Kitagawa, M., Y. Matsumura, and T. Tsuchido. 2000. Small heat shock proteins, IbpA and IbpB, are involved in resistances to heat and superoxide stresses in Escherichia coli. FEMS Microbiol. Lett. 184:165-171.
23. Kovach, M. E., et al. 1995. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-176.
24. Kuczyńska-Wiśnik, D., et al. 2002. The Escherichia coli small heat-shock proteins IbpA and IbpB prevent the aggregation of endogenous proteins denatured in vivo during extreme heat shock. Microbiology 148:1757-1765.
25. Lee, S. Y., Y. K. Lee, and H. N. Chang. 1995. Stimulatory effects of amino acids and oleic acid on poly(3-hydroxybutyric acid) synthesis by recombinant Escherichia coli. J. Ferment. Bioeng. 79:177-180.
26. Madison, L. L., and G. W. Huisman. 1999. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 63:21-53.
27. Mecsas, J., P. E. Rouviere, J. W. Erickson, T. J. Donohue, and C. A. Gross. 1993. The activity of sigma E, an Escherichia coli heat-inducible sigma-factor, is modulated by expression of outer membrane proteins. Genes Dev. 7:2618-2628.
28. Miller, J. H. 1992. A short course in bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
29. Neidhardt, F. C., et al. (ed.). 1996. Escherichia coli and Salmonella, cellular and molecular biology, 2nd ed. American Society for Microbiology, Washington, DC.
30. Nikel, P. I., A. de Almeida, E. C. Melillo, M. A. Galvagno, and M. J. Pettinari. 2006. New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products. Appl. Environ. Microbiol. 72:3949-3954.
31. Obrist, M., and F. Narberhaus. 2005. Identification of a turnover element in region 2.1 of Escherichia coli sigma32 by a bacterial one-hybrid approach. J. Bacteriol. 187:3807-3813.
32. Overath, P., H. U. Schairer, and W. Stoffel. 1970. Correlation of in vivo and in vitro phase transitions of membrane lipids in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 67:606-612.
33. Pettinari, J. M., L. Chaneton, G. Vázquez, A. Steinbüchel, and B. S. Méndez. 2003. Insertion sequence-like elements associated with putative polyhydroxybutyrate regulatory genes in Azotobacter sp. FA8. Plasmid 50:36-44.
34. Pettinari, M. J., et al. 2001. Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8. Appl. Environ. Microbiol. 67:5331-5334.
35. Pötter, M., et al. 2004. The complex structure of polyhydroxybutyrate (PHB) granules: four orthologous and paralogous phasins occur in Ralstonia eutropha. Microbiology 150:2301-2311.
36. Pötter, M., and A. Steinbüchel. 2005. Poly(3-hydroxybutyrate) granule-associated proteins: impacts on poly(3-hydroxybutyrate) synthesis and degradation. Biomacromolecules 6:552-560.
37. Rhodius, V. A., and J. T. Wade. 2009. Technical considerations in using DNA microarrays to define regulons. Methods 47:63-72.
38. Rutherford, B. J., et al. 2010. Functional genomic study of exogenous n-butanol stress in Escherichia coli. Appl. Environ. Microbiol. 76:1935-1945.
39. Straus, D. B., W. A. Walter, and C. A. Gross. 1987. The heat shock response of E. coli is regulated by changes in the concentration of sigma 32. Nature 329:348-351.
40. Tessmer, N., et al. 2007. Heat-shock protein HspA mimics the function of phasins sensu stricto in recombinant strains of Escherichia coli accumulating polythioesters or polyhydroxyalkanoates. Microbiology 153:366-374.
41. Tetsch, L., C. Koller, I. Haneburger, and K. Jung. 2008. The membraneintegrated transcriptional activator CadC of Escherichia coli senses lysine indirectly via the interaction with the lysine permease LysP. Mol. Microbiol. 67:570-583.
42. Valdez-Cruz, N. A., L. Caspeta, N. O. Perez, O. T. Ramirez, and M. A. Trujillo-Roldan. 2010. Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters. Microb. Cell Fact. 9:18.
43. Wieczorek, R., A. Pries, A. Steinbüchel, and F. Mayer. 1995. Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. J. Bacteriol. 177:2425-2435.
44. Wyborn, N. R., et al. 2002. Expression of the Escherichia coli yfiD gene responds to intracellular pH and reduces the accumulation of acidic metabolic end products. Microbiology 148:1015-1026.
45. York, G. M., J. Stubbe, and A. J. Sinskey. 2002. The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J. Bacteriol. 184:59-66.