Expression of the Escherichia coli yfiD gene responds to intracellular pH and reduces the accumulation of acidic metabolic end products

Microbiology

Volumn 148, Issue 4, 2002, Pages 1015-1026

  Wyborn, Neil R ^a   Messenger, Sarah L ^a   Henderson, Robin A ^a   Sawers, Gary ^a   Roberts, Ruth E ^a   Attwood, Margaret M ^a   Green, Jeffrey ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Acid stress; Formate; pH homeostasis; Pyruvate formate lyase; Radical protein

Indexed keywords

ALTEPLASE; BACTERIAL PROTEIN; BETA GALACTOSIDASE; CARBOXYLIC ACID; DIMER; FORMIC ACID; LYASE; OXYGEN; PROTEIN YFID; PYRUVIC ACID; RADICAL; REGULATOR PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; CELL PH; CONTROLLED STUDY; ESCHERICHIA COLI; GENE ACTIVATION; GENE ACTIVITY; GENE EXPRESSION REGULATION; GENE FUSION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DEGRADATION; REPORTER GENE; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE;

ARCA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0036231445     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-148-4-1015     Document Type: Article

References (36)

1. Structure and mechanism of the glycyl radical enzyme pyruvate formate-lyase
2. Determination of D-glucose with glucose oxidase and peroxidase
3. Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis
4. Cytochrome o (cyoABCDE) and d (cydAB) oxidase gene expression in Escherichia coli is regulated by oxygen, pH and the fur gene product
5. Pressure-induced inactivation of E. coli β-galactosidase: Influence of pH and temperature
6. Protein phosphorylation in Escherichia coli L-form NC-7
7. HlyX, the FNR homologue of Actinobacillus pleuropneumoniae, is a [4Fe 4S]-containing oxygen-responsive transcription regulator that anaerobically activates FNR-dependent class I promoters via an enhanced AR1-contact
8. Identification of a surface of FNR overlapping AR1 that is required for repression of gene expression
9. Downregulation of Escherichia coli yfiD expression by FNR occupying a site at -93.5 involves the AR1-containing surface of FNR
10. Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase
11. New method for generating deletions and gene replacements in Escherichia coli
12. Proteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate)
13. Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate
14. Regulated expression in vitro of genes coding for formate hydrogenlyase components of Escherichia coli
15. FNR is a direct oxygen sensor having a biphasic response curve
16. Ultrastructure and pyruvate formate-lyase radical quenching property of the multimeric AdhE protein of Escherichia coli
17. Oxygen sensing by the global regulator FNR: The role of the iron-sulfur cluster
18. DNA-binding and dimerization of the Fe-S containing FNR protein Escherichia coli are regulated by oxygen
19. A novel promoter architecture for microaerobic activation by the anaerobic transcription factor FNR
20. Nucleotide sequence of the Escherichia coli cad operon: A system for neutralization of low extracellular pH
21. Assay of β-galactosidase
22. Roles of LysP and CadC in mediating the lysine requirement for acid induction of the Escherichia coli cad operon
23. Protein patterns of gel-entrapped Escherichia coli cells differ from those of free floating organisms
24. Mössbauer spectroscopy as a tool for the study of activation/inactivation of the transcription regulator FNR in whole cells of Escherichia coli
25. Purification, characterization and mode of action of PdhR, the transcriptional repressor of the pdhR-aceEF-lpd operon of Escherichia coli
26. Mechanism of regulation of the formate-hydrogenlyase pathway by oxygen, nitrate, and pH: Definition of the formate regulon
27. Specific transcriptional requirements for positive regulation of the anaerobically inducible pflB operon by ArcA and FNR
28. Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12
29. A glycyl radical solution: Oxygen-dependent interconversion of pyruvate formate-lyase
30. p-Hydroxyphenylacetate decarboxylase from Clostridium difficile
31. pH-regulated genes and survival at extreme pH
32. The Thiobacilli
33. The free radical in pyruvate formate-lyase is located on glycine-734
34. YfiD of Escherichia coli and Y061 of bacteriophage T4 as autonomous glycyl radical cofactors reconstituting the catalytic center of oxygen-fragmented pyruvate formate-lyase
35. Spacing requirements for transcription activation by Escherichia coli