Rapid and sensitive detection of Pseudomonas aeruginosa in chlorinated water and aerosols targeting gyrB gene using real-time PCR

Journal of Applied Microbiology

Volumn 111, Issue 4, 2011, Pages 893-903

  Lee, C S ^a   Wetzel, K ^a   Buckley, T ^a   Wozniak, D ^b   Lee, J ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b THE OHIO STATE UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Aerosol; GyrB; Pseudomonas aeruginosa; Real time PCR; Water

Indexed keywords

BACTERIA; GENES; POLYMERASE CHAIN REACTION; POTABLE WATER; RISK ASSESSMENT; WATER LEVELS;

CHLORINATED WATERS; GYRB; GYRB GENES; PRIMER SETS; PS. AERUGINOSA; PSEUDOMONAS AERUGINOSA; RAPID DETECTION; REAL-TIME PCR; REAL-TIME PCR ASSAY; SENSITIVE DETECTION;

AEROSOLS;

DNA; DRINKING WATER; WATER;

AEROSOL COMPOSITION; ATMOSPHERIC POLLUTION; BACTERIUM; BIOASSAY; DISEASE TRANSMISSION; DRINKING WATER; GEL; NEUTRALIZATION; OPTIMIZATION; POLYMERASE CHAIN REACTION; REAL TIME; RISK ASSESSMENT; SAMPLING; WATER POLLUTION;

AEROSOL; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL TRANSMISSION; BACTERIUM DETECTION; BACTERIUM ISOLATE; COLONY FORMING UNIT; CYSTIC FIBROSIS; DIAGNOSTIC ACCURACY; DNA EXTRACTION; GENE TARGETING; HUMAN; LEGIONELLA PNEUMOPHILA; NEBULIZER; NONHUMAN; NUCLEOTIDE SEQUENCE; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; RISK ASSESSMENT;

BACTERIA (MICROORGANISMS); LEGIONELLA PNEUMOPHILA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 80052802532     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05107.x     Document Type: Article

References (49)

1. American Public Health Association (1992) Standard Methods for the Examination of Water and Wastewater. Washington, DC: American Public Health Association, Inc.
2. Anuj, S.N., Whiley, D.M., Kidd, T.J., Bell, S.C., Wainwright, C.E., Nissen, M.D. and Sloots, T.P. (2009) Identification of Pseudomonas aeruginosa by a duplex real-time polymerase chain reaction assay targeting the ecfX and the gyrB genes. Diagn Microbiol Infect Dis 63, 127-131.
3. Anzai, Y., Kim, H., Park, J.Y., Wakabayashi, H. and Oyaizu, H. (2000) Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563-1589.
4. Armstrong, D.S., Grimwood, K., Carzino, R., Carlin, J.B., Olinsky, A. and Phelan, P.D. (1995) Lower respiratory infection and inflammation in infants with newly diagnosed cystic fibrosis. BMJ 310, 1571-1572.
5. Bert, F., Maubec, E., Bruneau, B., Berry, P. and Lambert-Zechovsky, N. (1998) Multi-resistant Pseudomonas aeruginosa outbreak associated with contaminated tap water in a neurosurgery intensive care unit. J Hosp Infect 39, 53-62.
6. Berthelot, P., Grattard, F., Mahul, P., Pain, P., Jospe, R., Venet, C., Carricajo, A., Aubert, G. et al. (2001) Prospective study of nosocomial colonization and infection due to Pseudomonas aeruginosa in mechanically ventilated patients. Intensive Care Med 27, 503-512.
7. Buttner, M.P., Willeke, K. and Grinshpun, S.A. (1997) Sampling and analysis of airborne microorganism. In Manual of Environmental Microbiology ed. Hurst, C.J. and Knudsen, G.R. pp. 629-640. Washington, DC: American Society for Microbiology.
8. Cavalca, L., Di Gennaro, P., Colombo, M., Andreoni, V., Bernasconi, S., Ronco, I. and Bestetti, G. (2000) Distribution of catabolic pathways in some hydrocarbon-degrading bacteria from a subsurface polluted soil. Res Microbiol 151, 877-887.
9. Chandrasekar, P.H., Rolston, K.V., Kannangara, D.W., LeFrock, J.L. and Binnick, S.A. (1984) Hot tub-associated dermatitis due to Pseudomonas aeruginosa. Case report and review of the literature. Arch Dermatol 120, 1337-1340.
10. Cobben, N.A., Drent, M., Jonkers, M., Wouters, E.F., Vaneechoutte, M. and Stobberingh, E.E. (1996) Outbreak of severe Pseudomonas aeruginosa respiratory infections due to contaminated nebulizers. J Hosp Infect 33, 63-70.
11. De Vos, D., Lim, A. Jr, Pirnay, J.P., Struelens, M., Vandenvelde, C., Duinslaeger, L. et al. (1997) Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL. J Clin Microbiol 35, 1295-1299.
12. Deschaght, P., De Baere, T., Van Simaey, L., Van Daele, S., De Baets, F., De Vos, D. et al. (2009) Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients. BMC Microbiol 9, 244.
13. Doring, G., Ulrich, M., Muller, W., Bitzer, J., Schmidt-Koenig, L., Munst, L. et al. (1991) Generation of Pseudomonas aeruginosa aerosols during handwashing from contaminated sink drains, transmission to hands of hospital personnel, and its prevention by use of a new heating device. Zentralbl Hyg Umweltmed 191, 494-505.
14. Emde, K.M.E., Smith, D.W. and Facey, R. (1992) Initial investigation of microbially influenced corrosion (MIC) in a low-temperature water distribution-system. Water Res 26, 169-175.
15. Feizabadi, M.M., Majnooni, A., Nomanpour, B., Fatolahzadeh, B., Raji, N., Delfani, S. et al. (2010) Direct detection of Pseudomonas aeruginosa from patients with healthcare associated pneumonia by real time PCR. Infect Genet Evol 10, 1247-1251.
16. Ferroni, A., Nguyen, L., Pron, B., Quesne, G., Brusset, M.C. and Berche, P. (1998) Outbreak of nosocomial urinary tract infections due to Pseudomonas aeruginosa in a paediatric surgical unit associated with tap-water contamination. J Hosp Infect 39, 301-307.
17. Fukushima, M., Kakinuma, K. and Kawaguchi, R. (2002) Phylogenetic analysis of Salmonella, Shigella, and Escherichia coli strains on the basis of the gyrB gene sequence. J Clin Microbiol 40, 2779-2785.
18. Granstrom, M., Ericsson, A., Strandvik, B., Wretlind, B., Pavlovskis, O.R., Berka, R. and Vasil, M.L. (1984) Relation between antibody response to Pseudomonas aeruginosa exoproteins and colonization/infection in patients with cystic fibrosis. Acta Paediatr Scand 73, 772-777.
19. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95-98.
20. Hunter, P.R. (1993) The microbiology of bottled natural mineral waters. J Appl Bacteriol 74, 345-352.
21. Karadzic, I., Masui, A., Zivkovic, L.I. and Fujiwara, N. (2006) Purification and characterization of an alkaline lipase from Pseudomonas aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid. J Biosci Bioeng 102, 82-89.
22. Kasai, H., Watanabe, K., Gasteiger, E., Bairoch, A., Isono, K., Yamamoto, S. and Harayama, S. (1998) Construction of the gyrB database for the identification and classification of bacteria. Genome Inform Ser Workshop Genome Inform 9, 13-21.
23. Khan, A.A. and Cerniglia, C.E. (1994) Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR. Appl Environ Microbiol 60, 3739-3745.
24. Khan, I.U. and Yadav, J.S. (2004) Real-time PCR assays for genus-specific detection and quantification of culturable and non-culturable mycobacteria and pseudomonads in metalworking fluids. Mol Cell Probes 18, 67-73.
25. Kimata, N., Nishino, T., Suzuki, S. and Kogure, K. (2004) Pseudomonas aeruginosa isolated from marine environments in Tokyo Bay. Microb Ecol 47, 41-47.
26. Lan, J., Zhang, X.H., Wang, Y., Chen, J. and Han, Y. (2008) Isolation of an unusual strain of Edwardsiella tarda from turbot and establish a PCR detection technique with the gyrB gene. J Appl Microbiol 105, 644-651.
27. Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H. et al. (2007) Clustal W and Clustal×version 2.0. Bioinformatics 23, 2947-2948.
28. Lavenir, R., Jocktane, D., Laurent, F., Nazaret, S. and Cournoyer, B. (2007) Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecfX gene target. J Microbiol Methods 70, 20-29.
29. Lee, J. and Deininger, R.A. (2010) Real-time determination of the efficacy of residual disinfection to limit wastewater contamination in a water distribution system using filtration-based luminescence. Water Environ Res 82, 475-478.
30. Lee, C.S. and Lee, J. (2010) Evaluation of new gyrB-based real-time PCR system for the detection of B. fragilis as an indicator of human-specific fecal contamination. J Microbiol Methods 82, 311-318.
31. Moore, E.R.B., Mau, M., Arnscheidt, A., Bottger, E.C., Hutson, R.A., Collins, M.D. et al. (1996) The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto) and estimation of the natural intrageneric relationships. Syst Appl Microbiol 19, 478-492.
32. Motoshima, M., Yanagihara, K., Fukushima, K., Matsuda, J., Sugahara, K., Hirakata, Y. et al. (2007) Rapid and accurate detection of Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis targeting gyrB gene. Diagn Microbiol Infect Dis 58, 53-58.
33. Naze, F., Jouen, E., Randriamahazo, R.T., Simac, C., Laurent, P., Bleriot, A. et al. (2010) Pseudomonas aeruginosa outbreak linked to mineral water bottles in a neonatal intensive care unit: fast typing by use of high-resolution melting analysis of a variable-number tandem-repeat locus. J Clin Microbiol 48, 3146-3152.
34. Pellett, S., Bigley, D.V. and Grimes, D.J. (1983) Distribution of Pseudomonas aeruginosa in a riverine ecosystem. Appl Environ Microbiol 45, 328-332.
35. Qin, X., Emerson, J., Stapp, J., Stapp, L., Abe, P. and Burns, J.L. (2003) Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. J Clin Microbiol 41, 4312-4317.
36. Quittner, A.L., Modi, A.C., Wainwright, C., Otto, K., Kirihara, J. and Montgomery, A.B. (2009) Determination of the minimal clinically important difference scores for the cystic fibrosis questionnaire-revised respiratory symptom scale in two populations of patients with cystic fibrosis and chronic Pseudomonas aeruginosa airway infection. Chest 135, 1610-1618.
37. R Development Core Team (2009) R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing.
38. Rasmussen, R. (2001) Quantification on the LightCycler. In Rapid Cycle Real-time PCR, Methods and Applications ed. Meuer, S., Wittwer, C. and Nakagawara, K. pp. 21-34. Heidelberg: Springer Press.
39. Reid, T.M. and Porter, I.A. (1981) An outbreak of otitis externa in competitive swimmers due to Pseudomonas aeruginosa. J Hyg (Lond) 86, 357-362.
40. Relman, D.A., Schmidt, T.M., MacDermott, R.P. and Falkow, S. (1992) Identification of the uncultured bacillus of Whipple's disease. N Engl J Med 327, 293-301.
41. Rutala, W.A. and Weber, D.J. and the Healthcare Infection Control Practices Advisory Committee (HICPAC) (2008) Guideline for disinfection and sterilization in healthcare facilities, 2008. Available at: (accessed on 6 June 2011).
42. Schwartz, T., Volkmann, H., Kirchen, S., Kohnen, W. Schon-Holz, K., Jansen, B. and Obst, U. (2006) Real-time PCR detection of Pseudomonas aeruginosa in clinical and municipal wastewater and genotyping of the ciprofloxacin-resistant isolates. FEMS Microbiol Ecol 57, 158-167.
43. da Silva Filho, L.V., Tateno, A.F., Velloso Lde, F., Levi, J.E., Fernandes, S., Bento, C.N. et al. (2004) Identification of Pseudomonas aeruginosa, Burkholderia cepacia complex, and Stenotrophomonas maltophilia in respiratory samples from cystic fibrosis patients using multiplex PCR. Pediatr Pulmonol 37, 537-547.
44. Subrayan, V., Peyman, M., Lek Yap, S., Mohamed Ali, N.A. and Devi, S. (2010) Assessment of polymerase chain reaction in the detection of Pseudomonas aeruginosa in contact lens-induced severe infectious keratitis. Eye Contact Lens 36, 201-203.
45. Trautmann, M., Michalsky, T., Wiedeck, H., Radosavljevic, V. and Ruhnke, M. (2001) Tap water colonization with Pseudomonas aeruginosa in a surgical intensive care unit (ICU) and relation to Pseudomonas infections of ICU patients. Infect Control Hosp Epidemiol 22, 49-52.
46. Valles, J., Mariscal, D., Cortes, P., Coll, P., Villagra, A., Diaz, E. et al. (2004) Patterns of colonization by Pseudomonas aeruginosa in intubated patients: a 3-year prospective study of 1, 607 isolates using pulsed-field gel electrophoresis with implications for prevention of ventilator-associated pneumonia. Intensive Care Med 30, 1768-1775.
47. West, S.E., Zeng, L., Lee, B.L., Kosorok, M.R., Laxova, A., Rock, M.J. et al. (2002) Respiratory infections with Pseudomonas aeruginosa in children with cystic fibrosis: early detection by serology and assessment of risk factors. JAMA 287, 2958-2967.
48. Wolfgang, M.C., Kulasekara, B.R., Liang, X., Boyd, D., Wu, K., Yang, Q. et al. (2003) Conservation of genome content and virulence determinants among clinical and environmental isolates of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 100, 8484-8489.
49. Yamamoto, S., Kasai, H., Arnold, D.L., Jackson, R.W., Vivian, A. and Harayama, S. (2000) Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. Microbiology 146, 2385-2394.