Rapid and accurate detection of Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis targeting gyrB gene

Diagnostic Microbiology and Infectious Disease

Volumn 58, Issue 1, 2007, Pages 53-58

  Motoshima, Maiko ^a   Yanagihara, Katsunori ^a   Fukushima, Kazuko ^a   Matsuda, Junichi ^a   Sugahara, Kazuyuki ^a   Hirakata, Yochi ^a   Yamada, Yasuaki ^a   Kohno, Shigeru ^a,b   Kamihira, Shimeru ^a  

^a NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES   (Japan)

^b NAGASAKI UNIVERSITY HOSPITAL   (Japan)

Author keywords

Gyrase B; Melting temperature; Pseudomonas; Taxonomy

Indexed keywords

DNA TOPOISOMERASE (ATP HYDROLYSING) B;

ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; GENE TARGETING; GRAM NEGATIVE BACTERIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS OTITIDIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; DNA GYRASE; DNA PRIMERS; DNA, BACTERIAL; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; TRANSITION TEMPERATURE;

EID: 34247877808     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2006.11.007     Document Type: Article

References (15)

1. Anzai Y., Kim H., Park J.Y., Wakabayashi H., and Oyaizu H. Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int. J. Syst. Evol. Microbiol. 50 (2000) 1563-1589
2. Clark L.L., Dajcs J.J., McLean C.H., Bartell J.G., and Stroman D.W. Pseudomonas otitidis sp. nov., isolated from patients with otic infections. Int. J. Syst. Evol. Microbiol. 56 (2006) 709-714
3. Clarke L., Moore J.E., Millar B.C., Garske L., Xu J., Heuzenroeder M.W., Crowe M., and Elborn J.S. Development of a diagnostic PCR assay that targets a heat-shock protein gene (groES) for detection of Pseudomonas spp. in cystic fibrosis patients. J. Med. Microbiol. 52 (2003) 759-763
4. Davis J.R., Stager C.E., and Araj G.F. 4-h Identification of Pseudomonas aeruginosa with 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan. J. Clin. Microbiol. 17 (1983) 1054-1056
5. De Vos D., Lim A.J., Pirnay J.P., Struelens M., Vandenvelde C., Duinslaeger L., Vanderkelen A., and Cornelis P. Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL. J. Clin. Microbiol. 35 (1997) 1295-1299
6. Funke G., and Funke-Kissling P. Evaluation of the new VITEK 2 card for identification of clinically relevant gram-negative rods. J. Clin. Microbiol. 42 (2004) 4067-4071
7. Kasai H., Watanabe K., Gasteiger E., Bairoch A., Isono K., Yamamoto S., and Harayama S. Construction of the gyrB database for the identification and classification of bacteria. Genome Inform. Ser. Workshop Genome Inform. 9 (1998) 13-21
8. Khan A.A., and Cerninglia C.E. Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR. Appl. Environ. Microbiol. 60 (1994) 3739-3745
9. Kiska D.L., and Gilligan P.H. Pseudomonas: Manual of Clinical Microbiology. 8th ed. In: Murphy P.R. (Ed) (2003), ASMpress, Washington (DC) 719-728
10. Kurupati P., Kumarasinghe G., and Laa Poh C. Direct identification of Pseudomonas aeruginosa from blood culture bottles by PCR-enzyme liked immunosorbent assay using oprI gene specific primers. Mol. Cell. Probes 19 (2005) 417-421
11. Pollack M. Pseudomonas aeruginosa. In: Mandell G.L., Bennett J.E., and Dolin R. (Eds). Principles and Practice of Infections Diseases. 5th ed. (2000), Churchill Livingstone Inc, New York (NY) 1980-2003
12. Qin X., Emerson J., Stapp J., Stapp L., Abe P., and Burns J.L. Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. J. Clin. Microbiol. 41 (2003) 4312-4317
13. Sulzinski M.A., Moorman G.W., Schlagnhaufer B., and Romaine C.P. A simple DNA extraction method for PCR-based detection of Xanthomonas campestris pv. pelargonii in geraniums. J. Phytopathol. 145 (1997) 213-215
14. Vandamme P., Pot B., Gillis M., De Vos P., Kersters K., and Swings J. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol. Rev. 60 (1996) 407-438
15. Vandamme P.A.R. In: Murphy P.R. (Ed). Taxonomy and Classification of Bacteria: Manual of Clinical Microbiology. 8th ed. (2003), ASMpress, Washington (DC) 271-286