Patterns of colonization by Pseudomonas aeruginosa in intubated patients: A 3-year prospective study of 1,607 isolates using pulsed-field gel electrophoresis with implications for prevention of ventilator-associated pneumonia

Intensive Care Medicine

Volumn 30, Issue 9, 2004, Pages 1768-1775

  Vallés, Jordi ^a   Mariscal, Dolors ^a   Cortés, Pilar ^a   Coll, Pere ^a,b   Villagrá, Ana ^a   Díaz, Emili ^a   Artigas, Antonio ^a   Rello, Jordi ^c  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b Sant Pau University Hospital   (Spain)

^c HOSPITAL UNIVERSITARI JOAN XXIII   (Spain)

Author keywords

Colonization; Intensive care; Mechanical ventilation; Pneumonia; Pseudomonas aeruginosa

Indexed keywords

TAP WATER;

ADULT; AGED; ARTICLE; ARTIFICIAL VENTILATION; BACTERIAL COLONIZATION; BACTERIUM CONTAMINATION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; DISEASE ASSOCIATION; ERADICATION THERAPY; FEMALE; GLOTTIS; HUMAN; INFECTION CONTROL; INTENSIVE CARE; INTUBATION; LUNG INFECTION; MAJOR CLINICAL STUDY; MALE; OBSERVATION; OROPHARYNX; PNEUMONIA; PROSPECTIVE STUDY; PSEUDOMONAS AERUGINOSA; PULSED FIELD GEL ELECTROPHORESIS; RECTUM; RESPIRATORY TRACT INFECTION; STOMACH; STRAIN IDENTIFICATION; TEACHING HOSPITAL; TRACHEA; VENTILATOR ASSOCIATED PNEUMONIA; WATER SAMPLING;

AGED; CROSS INFECTION; ELECTROPHORESIS, GEL, PULSED-FIELD; FEMALE; HOSPITALS; HUMANS; INTENSIVE CARE UNITS; INTUBATION; MALE; MIDDLE AGED; PNEUMONIA, BACTERIAL; PROSPECTIVE STUDIES; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; RESPIRATION, ARTIFICIAL;

EID: 4644299588     PISSN: 03424642     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00134-004-2382-6     Document Type: Article

References (26)

1. Rello J, Rué M, Jubert P, Muses G, Sonora R, Vallés J, Niederman MS (1997) Survival in patients with nosocomial pneumonia: impact of the severity of illness and the etiologic agent. Crit Care Med 25:1862-1867
2. Brewer SC, Wunderink RG, Jones CB, Leeper KV (1996) Ventilator-associated pneumonia due to Pseudomonas aeruginosa. Chest 109:1019-1029
3. National Nosocomial Infections Surveillance System (1999) Data summary from January 1990-May 1999, issued June 1999. Am J Infect Control 27:520-532
4. Talon D, Mulin B, Rouget C, Bailly P, Thouverez M, Viel JF (1998) Risks and routes for ventilator-associated pneumonia with Pseudomonas aeruginosa. Am J Respir Crit Care Med 157:978-984
5. Bergmans DC, Bonten MJ, van Tiel FH, Gaillard CA, van der GS, Wilting RM, de Leeuw PW, Stobberingh EE (1998) Cross-colonisation with Pseudomonas aeruginosa of patients in an intensive care unit. Thorax 53:1053-1058
6. Knaus WA, Draper EA, Wagner DP, Zimmerman JE (1985) APACHE II: a severity of disease classification system. Crit Care Med 13:818-829
7. Chastre J, Fagon JY, Trouillet JL (1995) Diagnosis and treatment of nosocomial pneumonia in patients in intensive care units. Clin Infect Dis 21 [Suppl 3]:S226-S237
8. El-Ebiary M, Torres A, González J, Puig de la Bellacasa J, García C, Jiménez de Anta MT, Ferrer M, Rodríguez-Roisin R (1993) Quantitative cultures of endotracheal aspirates for the diagnosis of ventilator-associated pneumonia. Am Rev Respir Dis 148:1552-1557
9. Gilchrist MJR (1992) Microbiological culturing of environmental and medical-device surfaces. In: Isenberg HD (ed) Clinical microbiology procedures handbook. American Society for Microbiology, Washington
10. Cortés P, Mariscal D, Vallés J, Rello J, Coll P (2001) Implications of the presence of polyclonal Pseudomonas aeruginosa in an intensive care unit. A 27-month prospective study on molecular epidemiology. Infect Control Hosp Epidemiol 22:720-723
11. Tenover FC, Arbeit RD, Goring RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233-2239
12. Döring G, Hörz M, Ortelt J, Grupp H, Wolz C (1995) Molecular epidemiology of Pseudomonas aeruginosa in an intensive care unit. Epidemiol Infect 110:427-436
13. Olson B, Weinstein RA, Nathan C, Chamberlin W, Kabins SA (1984) Epidemiology of endemic Pseudomonas aeruginosa: Why infection control efforts have failed. J Infect Dis 150:808-816
14. Speijer H, Savelkoul PH, Bonten MJ, Stobberingh EE, Tjhie JH (1999) Application of different genotyping methods for Pseudomonas aeruginosa in a setting of endemicity in an intensive care unit. J Clin Microbiol 37:3654-3661
15. Bertrand X, Thouverez M, Talon D, Boillot A, Capellier G, Floriot C, Helias JP (2001) Endemicity, molecular diversity and colonisation routes of Pseudomonas aeruginosa in intensive care units. Intensive Care Med 27:1263-1268
16. Bonten MJ, Bergmans DC, Speijer H, Stobberingh EE (1999) Characteristics of polyclonal endemicity of Pseudomonas aeruginosa colonization in intensive care units. Implications for infection control. Am J Respir Crit Care Med 160:1212-1219
17. Trautmann M, Michalsky T, Wiedeck H, Radosavljevic V, Ruhnke M (2001) Tap water colonization with Pseudomonas aeruginosa in a surgical intensive care unit (ICU) and relation to Pseudomonas infections of ICU patients. Infect Control Hosp Epidemiol 22:49-52
18. Bert F, Maubec E, Bruneau B, Berry P, Lambert-Zechovsky N (1998) Multi-resistant Pseudomonas aeruginosa outbreak associated with contaminated tap water in a neurosurgery intensive care unit. J Hosp Infect 39:53-62
19. Ferroni A, Nguyen L, Pron B, Quesne G, Brusset MC, Berche P (1998) Outbreak of nosocomial urinary tract infections due to Pseudomonas aeruginosa in a pediatric surgical unit associated with ta-water contamination. J Hosp Infect 39:301-307
20. Reuter S, Sigge A, Wiedeck H, Trautmann M (2002) Analysis of transmission pathways of Pseudomonas aeruginosa between patients and tap water outlets. Crit Care Med 30:2222-2228
21. Bonten MJ, Gaillard CA, van Tiel FH, Smeets HGW, van der Geest S, Stobberingh EE (1994) The stomach is not a source for colonization of the upper respiratory tract and pneumonia in ICU patients. Chest 105:878-884
22. Hauser AR, Cobb E, Bodí M, Mariscal D, Vallés J, Engel JN, Rello J (2002) Type III protein secretion is associated with poor clinical outcomes in patients with ventilator-associated pneumonia caused by Pseudomonas aeruginosa. Crit Care Med 30:521-528
23. Roy-Burman A, Savel RH, Racine S, Swanson BL, Revadigar NS, Fujimoto J, Sawa T, Frank DW, Wiener-Kronish JP (2001) Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections. J Infect Dis 183:1767-1774
24. Pittet D (2001) Compliance with hand disinfection and its impact on hospital-acquired infections. J Hosp Infect 48 [Suppl A]:S40-S46
25. Feldman C, Kassel M, Cantrell J, Kaka S, Morar R, Goolam MA, Philips JI (1999) The presence and sequence of endotracheal tube colonization in patients undergoing mechanical ventilation. Eur Respir J 13:546-551
26. Foca M, Jakob K, Whittier S, Della LP, Factor S, Rubenstein D, Saiman L (2000) Endemic Pseudomonas aeruginosa infection in a neonatal intensive care unit. N Engl J Med 343:695-700