Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by loop-mediated isothermal amplification (LAMP)

PLoS Neglected Tropical Diseases

Volumn 5, Issue 8, 2011, Pages

  Grab, Dennis J ^a   Nikolskaia, Olga V ^a   Inoue, Noboru ^b   Thekisoe, Oriel M M ^c   Morrison, Liam J ^d   Gibson, Wendy ^e   Dumler, J Stephen ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

^c UNIVERSITY OF THE FREE STATE   (South Africa)

^d UNIVERSITY OF GLASGOW   (United Kingdom)

^e UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; PROTOZOAL DNA; TRITON X 100; DETERGENT; PRIMER DNA;

ANALYTICAL EQUIPMENT; ARTICLE; BLOOD; CEREBROSPINAL FLUID; CONTROLLED STUDY; DILUTION; DNA DETERMINATION; DNA EXTRACTION; ENVIRONMENTAL TEMPERATURE; FILTER PAPER; HUMAN; INCUBATION TIME; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MICROORGANISM DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE LOAD; SENSITIVITY ANALYSIS; TRYPANOSOMA RHODESIENSE; AFRICAN TRYPANOSOMIASIS; AGAR GEL ELECTROPHORESIS; ANIMAL; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; PARASITOLOGY; SENSITIVITY AND SPECIFICITY;

ANIMALS; DETERGENTS; DNA PRIMERS; DNA, PROTOZOAN; ELECTROPHORESIS, AGAR GEL; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SENSITIVITY AND SPECIFICITY; TRYPANOSOMA BRUCEI RHODESIENSE; TRYPANOSOMIASIS, AFRICAN;

EID: 80052418506     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0001249     Document Type: Article

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