Confocal laser scanning microscopy as an analytical tool in chromatographic research

Bioprocess and Biosystems Engineering

Volumn 31, Issue 3, 2008, Pages 241-259

  Hubbuch, Jürgen ^a   Kula, Maria Regina ^b  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Chromatography; Confocal laser scanning microscopy; Fluorescent techniques; Protein profiling

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; CONFOCAL MICROSCOPY; PARAMETER ESTIMATION; SENSITIVITY ANALYSIS;

CHROMATOGRAPHIC ADSORBENTS; CONFOCAL LASER SCANNING MICROSCOPY;

PROTEINS;

ADSORBENT; BOVINE SERUM ALBUMIN; CYTOCHROME C; FLUORESCENT DYE; LYSOZYME; OVOTRANSFERRIN;

ANALYTICAL PARAMETERS; BLEACHING; CHROMATOGRAPHY; CONFOCAL LASER MICROSCOPY; CONJUGATION; ENVIRONMENTAL PARAMETERS; EXPERIMENTAL STUDY; FLUORESCENCE; PREDICTION; PRIORITY JOURNAL; SHORT SURVEY; SOLID;

ADSORPTION; ALGORITHMS; BIOTECHNOLOGY; CHROMATOGRAPHY; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; MICROSCOPY, CONFOCAL; MOLECULAR CONFORMATION; PHOTOBLEACHING; PROTEIN CONFORMATION; PROTEINS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE;

EID: 41049083881     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-008-0197-5     Document Type: Short Survey

References (75)

1. Pawley JB (1995) Handbook of biological confocal microscopy. Plenum Press, New York
2. Ljunglöf A, Hjorth R (1996) Confocal microscopy as a tool for studying protein adsorption to chromatographic matrices. J Chromatogr A 743:75-83
3. Ljunglöf A, Thommes J (1998) Visualising intraparticle protein transport in porous adsorbents by confocal microscopy. J Chromatogr A 813:387-395
4. Linden T, Ljunglof A, Kula MR, Thommes J (1999) Visualizing two-component protein diffusion in porous adsorbents by confocal scanning laser microscopy. Biotechnol Bioeng 65:622-630
5. Linden T, Ljunglof A, Hagel L, Kula MR, Thommes J (2002) Visualizing patterns of protein uptake to porous media using confocal scanning laser microscopy. Sep Sci Technol 37:1-32
6. Hubbuch J, Linden T, Knieps E, Thommes J, Kula MR (2002) Dynamics of protein uptake within the adsorbent particle during packed bed chromatography. Biotechnol Bioeng 80:359-368
7. Dziennik SR, Belcher EB, Barker GA, DeBergalis MJ, Fernandez SE, Lenhoff AM (2003) Nondiffusive mechanisms enhance protein uptake rates in ion exchange particles. Proc Natl Acad Sci USA 100:420-425
8. Ahmed M, Pyle DL (1999) Investigation of single protein adsorption on ion exchangers using confocal laser scanning microscopy. J Chem Technol Biotechnol 74:193-198
9. Kasche V, de Boer M, Lazo C, Gad M (2003) Direct observation of intraparticle equilibration and the rate-limiting step in adsorption of proteins in chromatographic adsorbents with confocal laser scanning microscopy. J Chromatogr B Anal Technol Biomed Life Sci 790:115-129
10. Hubbuch J, Linden T, Knieps E, Ljunglof A, Thommes J, Kula MR (2003) Mechanism and kinetics of protein transport in chromatographic media studied by confocal laser scanning microscopy-part I. The interplay of sorbent structure and fluid phase conditions. J Chromatogr A 1021:93-104
11. Hubbuch R, Linden T, Knieps E, Thommes J, Kula MR (2003) Mechanism and kinetics of protein transport in chromatographic media studied by confocal laser scanning microscopy-part II. Impact on chromatographic separations. J Chromatogr A 1021:105-115
12. Suh CW, Kim MY, Choo JB, Kim JK, Kim HK, Lee EK (2004) Analysis of protein adsorption characteristics to nano-pore silica particles by using confocal laser scanning microscopy. J Biotechnol 112:267-277
13. Subramanian A, Hommerding J (2005) The use of confocal laser scanning microscopy to study the transport of biornacromolecules in a macroporous support. J Chromatogr B Anal Technol Biomed Life Sci 818:89-97
14. Shi QS, Zhou Y, Sun Y (2005) Influence of pH and ionic strength on the steric mass-action model parameters around the isoelectric point of protein. Biotechnol Progr 21:516-523
15. Russell SM, Carta G (2005) Multicomponent protein adsorption in supported cationic polyacrylamide hydrogels. AIChE J 51:2469-2480
16. Dziennik SR, Belcher EB, Barker GA, Lenhoff AM (2005) Effects of ionic strength on lysozyme uptake rates in cation exchangers. I: Uptake in SP sepharose FF. Biotechnol Bioeng 91:139-153
17. Zhou XP, Li W, Shi QH, Sun Y (2006) Analysis of mass transport models for protein adsorption to cation exchanger by visualization with confocal laser scanning microscopy. J Chromatogr A 1103:110-117
18. Teske CA, Schroeder M, Simon R, Hubbuch J (2005) Protein-labeling effects in confocal laser scanning microscopy. J Phys Chem B 109:13811-13817
19. Teske CA, von Lieres E, Schroder M, Ladiwala A, Cramer SM, Hubbuch JJ (2006) Competitive adsorption of labeled and native protein in confocal laser scanning microscopy. Biotechnol Bioeng 95:58-66
20. Siu SC, Boushaba R, Topoyassakul V, Graham A, Choudhury S, Moss G, Titchener-Hooker NJ (2006) Visualising fouling of a chromatographic matrix using confocal scanning laser microscopy. Biotechnol Bioeng 95:714-723
21. Katiyar A, Pinto NG (2006) Visualization of size-selective protein separations on spherical mesoporous silicates. Small 2:644-648
22. Harinarayan C, Mueller J, Ljunglof A, Fahrner R, Van Alstine J, van Reis R (2006) An exclusion mechanism in ion exchange chromatography. Biotechnol Bioeng 95:775-787
23. Yang K, Shi QH, Sun Y (2006) Modeling and simulation of protein uptake in cation exchanger visualized by confocal laser scanning microscopy. J Chromatogr A 1136:19-28
24. Susanto A, Herrmann T, Hubbuch J (2006) Short-cut method for the correction of light attenuation influences in the experimental data obtained from confocal laser scanning microscopy. J Chromatogr A 1136:29-38
25. Susanto A, Wekenborg K, Hubbuch J, Schmidt-Traub H (2006) Developing a chromatographic column model for bovine serum albumin on strong anion-exchanger Source30Q using data from confocal laser scanning microscopy. J Chromatogr A 1137:63-75
26. Ljunglöf A, Lacki K, Müller C, Harinarayan C, van Reis R, Fahrner R, Van Alstine J (2007) Ion exchange chromatography of antibody fragments. Biotechnol Bioeng 96:515-524
27. Teske CA, Simon R, Niebisch A, Hubbuch JJ (2007) Changes in retention behavior of fluorescently labeled proteins during ion-exchange chromatography caused by different protein surface labeling positions. Biotechnol Bioeng 98:193-200
28. Susanto A, Herrmann T, von Lieres E, Hubbuch JJ (2007) Investigation of pore diffusion hindrance of monoclonal antibody in hydrophobic interaction chromatography using confocal laser scanning microscopy. J Chromatogr A 1149:178-188
29. Tallarek U, Rapp E, Sann H, Reichl U, Seidel-Morgenstern A (2003) Quantitative study of electrokinetic transport in porous media by confocal laser scanning microscopy. Langmuir 19:4527-4531
30. Malmsten M, Xing KZ, Ljunglof A (1999) Confocal microscopy studies of trypsin immobilization on porous glycidyl methacrylate beads. J Colloid Interface Sci 220:436-442
31. Heinemann M, Wagner T, Doumeche B, Ansorge-Schumacher M, Buchs J (2002) A new approach for the spatially resolved qualitative analysis of the protein distribution in hydrogel beads based on confocal laser scanning microscopy. Biotechnol Lett 24:845-850
32. Heinemann M, Limper U, Buchs J (2004) New insights in the spatially resolved dynamic pH measurement in macroscopic large absorbent particles by confocal laser scanning microscopy. J Chromatogr A 1024:45-53
33. Tallarek U, Paces M, Rapp E (2003) Perfusive flow and intraparticle distribution of a neutral analyte in capillary electrochromatography. Electrophoresis 24:4241-4253
34. Martin C, Iberer G, Ubiera A, Carta G (2005) Two-component protein adsorption kinetics in porous ion exchange media. J Chromatogr A 1079:105-115
35. Buschmann V, Weston KD, Sauer M (2003) Spectroscopic study and evaluation of red-absorbing fluorescent dyes. Bioconjug Chem 14:195-204
36. Brinkley M (1992) A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents. Bioconjug Chem 3:2-13
37. Hermanson GT (1996) Bioconjugate techniques. Academic Press, New York
38. Anderson JR, Cherniavskaya O, Gitlin I, Engel GS, Yuditsky L, Whitesides GM (2002) Analysis by capillary electrophoresis of the kinetics of charge ladder formation for bovine carbonic anhydrase. Anal Chem 74:1870-1878
39. Jing P, Kaneta T, Imasaka T (2002) Micellar electrokinetic chromatography with diode laser-induced fluorescence detection as a tool for investigating the fluorescence labeling of proteins. Electrophoresis 23:550-555
40. Lin SH, Faller LD (2000) Preparation of Na,K-ATPase specifically modified on the anti-fluorescein antibody-inaccessible site by fluorescein 5′-isothiocyanate. Anal Biochem 287:303-312
41. Grunwaldt G, Haebel S, Spitz C, Steup M, Menzel R (2002) Multiple binding sites of fluorescein isothiocyanate moieties on myoglobin: photophysical heterogeneity as revealed by ground- and excited-state spectroscopy. J Photochem Photobiol B 67:177-186
42. Pinto D, Arriaga EA, Schoenherr RM, Chou SSH, Dovichi NJ (2003) Kinetics and apparent activation energy of the reaction of the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde with ovalbumin. J Chromatogr B Anal Technol Biomed Life Sci 793:107-114
43. Fureder-Kitzmuller E, Hesse J, Ebner A, Gruber HJ, Schutz GJ (2005) Non-exponential bleaching of single bioconjugated Cy5 molecules. Chem Phys Lett 404:13-18
44. Dismer F, Hubbuch J (2007) A novel approach to characterize the binding orientation of lysozyme on ion-exchange resins. J Chromatogr A 1149:312-320
45. Craig DB, Dovichi NJ (1998) Multiple labeling of proteins. Anal Chem 70:2493-2494
46. Richards DP, Stathakis C, Polakowski R, Ahmadzadeh H, Dovichi NJ (1999) Labeling effects on the isoelectric point of green fluorescent protein. J Chromatogr A 853:21-25
47. Stoyanov AV, Ahmadzadeh H, Krylov SN (2002) Heterogeneity of protein labeling with a fluorogenic reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde. J Chromatogr B Anal Technol Biomed Life Sci 780:283-287
48. Banks PR, Paquette DM (1995) Monitoring of a conjugation reaction between fluorescein isothiocyanate and myoglobin by capillary zone electrophoresis. J Chromatogr A 693:145-154
49. Kasche V, Buchtmann I (1981) A method for the isolation of homogeneous fluorescein-labeled proteins for studies on the variation in biological and fluorescence properties of the different conjugates. Hoppe Seylers Z Physiol Chem 362:1007-1012
50. Bingaman S, Huxley VH, Rumbaut RE (2003) Fluorescent dyes modify properties of proteins used in microvascular research. Microcirculation 10:221-231
51. Liu HJ, Strong RE, Krull IS, Cohen SA (2001) Homogeneous preparation of fluorescent-derivatized insulin and its application to competitive chromatographic immunoassays. Anal Biochem 298:103-111
52. Lu JZ, Zenobi R (1999) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying the composition of labeled proteins. Anal Biochem 269:312-316
53. Lu JZ, Zenobi R (2000) In-situ monitoring of protein labeling reactions by matrix-assisted laser desorption/ionization mass spectrometry. Fresenius J Anal Chem 366:3-9
54. Schnaible V, Przybylski M (1999) Identification of fluorescein-5′- isothiocyanate-modification sites in proteins by electrospray-ionization mass spectrometry. Bioconjug Chem 10:861-866
55. Shimura K, Karger BL (1994) Affinity probe capillary electrophoresis- analysis of recombinant human growth-hormone with a fluorescent-labeled antibody fragment. Anal Chem 66:9-15
56. Lebreton B, Lyddiatt A (2000) Application of aqueous two-phase partition to the production of homogeneous preparations of fluorescently labelled human serum albumin. J Chromatogr B Anal Technol Biomed Life Sci 743:263-269
57. Attiya S, Dickinson-Laing T, Cesarz J, Giese RD, Lee WE, Mah D, Harrison DJ (2002) Affinity protection chromatography for efficient labeling of antibodies for use in affinity capillary electrophoresis. Electrophoresis 23:750-758
58. Houen G, Olsen DT, Hansen PR, Petersen KB, Barkholt V (2003) Preparation of bioconjugates by solid-phase conjugation to ion exchange matrix-adsorbed carrier proteins. Bioconjug Chem 14:75-79
59. Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu JX, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugland RP, Haugland RP (2003) Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: Fluorescence of the dyes and their bioconjugates. J Histochem Cytochem 51:1699-1712
60. Gruber HJ, Hahn CD, Kada G, Riener CK, Harms GS, Ahrer W, Dax TG, Knaus HG (2000) Anomalous fluorescence enhancement of Cy3 and Cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin. Bioconjug Chem 11:696-704
61. Amneus H, Gabel D, Kasche V (1976) Resolution in affinity chromatography-effect of heterogeneity of immobilized soybean trypsin-inhibitor on separation of pancreatic proteases. J Chromatogr 120:391-397
62. Kasche V, Gottschlich N, Lindberg A, Niebuhrredder C, Schmieding J (1994) Perfusible and non-perfusible supports with monoclonal-antibodies for bioaffinity chromatography of Escherichia Coli penicillin amidase within its pH stability range. J Chromatogr A 660:137-145
63. Adenier A, Aaron JJ (2002) A spectroscopic study of the fluorescence quenching interactions between biomedically important salts and the fluorescent probe merocyanine 540. Spectrochim Acta A 58:543-551
64. Doose S, Neuweiler H, Sauer M (2005) A close look at fluorescence quenching of organic dyes by tryptophan. Chemphyschem 6:2277-2285
65. Linden T (2001) Untersuchungen zum inneren Transport bei der Proteinadsorption an poröse Medien mittels konfokaler Laser Raster Mikroskopie. Heinrich Heine University Düsseldorf, Düsseldorf
66. Bromley EHC, Hopkinson I (2002) Confocal microscopy of a dense particle system. J Colloid Interface Sci 245:75-80
67. Roerdink JBTM, Bakker M (1993) An Fft-based method for attenuation correction in fluorescence confocal microscopy. J Microsc Oxford 169:3-14
68. Visser TD, Groen FCA, Brakenhoff GJ (1991) Absorption and scattering correction in fluorescence confocal microscopy. J Microsc Oxford 163:189-200
69. Margadant F, Leemann T, Niederer P (1996) A precise light attenuation correction for confocal scanning microscopy with O(n(4/3)) computing time and O(n) memory requirements for n voxels. J Microsc Oxford 182:121-132
70. Stamnes JJ (1986) Waves in focal regions. Adam Hilger, Bristol
71. Schroder M, von Lieres E, Hubbuch J (2006) Direct quantification of intraparticle protein diffusion in chromatographic media. J Phys Chem B 110:1429-1436
72. Schroder M (2007) Untersuchungen zum Stofftransport von Proteinen in chromatographischen Medien mittels konfokaler Laser Raster Mikroskopie. University of Bielefeld, Bielefeld
73. Liapis AI, Grimes BA (2005) The coupling of the electrostatic potential with the transport and adsorption mechanisms in ion-exchange chromatography systems: theory and experiments. J Sep Sci 28:1909-1926
74. Dziennik SR (2002) Effects of ionic strength and stationary phase on protein adsorption and transport in ion-exchange particles. University of Delaware, Newark
75. Ljunglöf A (2002) Direct observation of biomolecule adsorption and spatial distribution of functional groups in chromatographic adsorbent particles. Uppsala University, Uppsala