Protein adsorption dynamics in cation-exchange chromatography quantitatively studied by confocal laser scanning microscopy

Chemical Engineering Science

Volumn 63, Issue 16, 2008, Pages 4045-4054

  Yang, Kun ^a   Bai, Shu ^a   Sun, Yan ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

Attenuation equation; Chromatography; Confocal laser scanning microscopy; Diffusion; Dynamics; Simulation

Indexed keywords

ABSORPTION; ADSORPTION; BODY FLUIDS; CHROMATOGRAPHIC ANALYSIS; CONFOCAL MICROSCOPY; DATA VISUALIZATION; DIFFUSION; DIFFUSION IN SOLIDS; DYNAMICS; EXPERIMENTS; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGERS; IONIC STRENGTH; IONIZATION OF LIQUIDS; LASER APPLICATIONS; LASERS; LIQUID PHASE EPITAXY; MARKOV PROCESSES; MASS TRANSFER; MAXWELL EQUATIONS; MICROSCOPIC EXAMINATION; PH; PH EFFECTS; POSITIVE IONS; PULSED LASER DEPOSITION; SCANNING; SEMICONDUCTOR DOPING; STEEL ANALYSIS;

(1 1 0) SURFACE; ACETATE BUFFERS; ADSORBENT PARTICLES; ADSORPTION BEHAVIOR; ADSORPTION DYNAMICS; ATTENUATION EQUATION; BATCH ADSORPTION; BOVINE SERUM ALBUMIN ADSORPTION; CATION EXCHANGE CHROMATOGRAPHY (CEC); CATION EXCHANGERS; CONCENTRATION PROFILES; CONFOCAL LASER SCANNING MICRO SCOPY (CLSM); DIFFUSIVE MASS-TRANSFER; ELSEVIER (CO); EXPERIMENTAL RESULTS; INTRA PARTICLE; LIQUID-PHASE; MASS TRANSFER MODELING; MATHEMATICAL MODELLING; MAXWELL-STEFAN MODELS; MICRO FLOW CELL; MODEL SIMULATIONS; PORE DIFFUSION; PORE DIFFUSION MODELS; PROTEIN ADSORPTION; PROTEIN CONCENTRATIONS; PROTEIN DISTRIBUTION; RESEARCH SYSTEM; SALT ADDITION; SALT CONCENTRATIONS; SEPHAROSE; STRENGTH (IGC: D5/D6); SURFACE DIFFUSION MODEL;

SURFACE DIFFUSION;

EID: 47849131140     PISSN: 00092509     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ces.2008.05.013     Document Type: Article

References (45)

1. Carta G., and Ubiera A. Particle-size distribution effects in batch adsorption. A.I.Ch.E. Journal 49 (2003) 3066-3073
2. Chang C., and Lenhoff A.M. Comparison of protein adsorption isotherms and uptake rates in preparative cation-exchange materials. Journal of Chromatography A 827 (1998) 281-293
3. Chen W.D., Dong X.Y., and Sun Y. Analysis of diffusion models for protein adsorption to porous anion-exchange adsorbent. Journal of Chromatography A 962 (2002) 29-40
4. Costa C., and Rodrigues A. Intraparticle diffusion of phenol in macroreticular adsorbents: modelling and experimental study of batch and CSTR adsorbers. Chemical Engineering Science 40 (1985) 983-993
5. Costa C., and Rodrigues A. Design of cyclic fixed-bed adsorption processes. Part I: phenol adsorption on polymeric adsorbents. A.I.Ch.E. Journal 31 (1985) 1645-1654
6. Dziennik S.R., Belcher E.B., Barker G.A., and Lenhoff A.M. Effects of ionic strength on lysozyme uptake rates in cation exchangers. I: uptake in SP Sepharose FF. Biotechnology and Bioengineering 91 (2005) 139-153
7. Gutenwik J., Nilsson B., and Axelsson A. Determination of protein diffusion coefficients in agarose gel with a diffusion cell. Biochemical Engineering Journal 19 (2004) 1-7
8. Gutenwik J., Nilsson B., and Axelsson A. Effect of hindered diffusion on the adsorption of proteins in agarose gel using a pore model. Journal of Chromatography A 1048 (2004) 161-172
9. Harinarayan C., Mueller J., Ljunglöf A., Fahrner R., Van Alstine J., and van Reis R. An exclusion mechanism in ion exchange chromatography. Biotechnology and Bioengineering 95 (2006) 775-787
10. He L.Z., Dong X.Y., and Sun Y. A diffusion model of protein and eluant for affinity filtration. Biochemical Engineering Journal 2 (1998) 53-62
11. Hubbuch J., and Kula M.R. Confocal laser scanning microscopy as an analytical tool in chromatographic research. Bioprocess Biosystem Engineering 31 (2008) 241-259
12. Hubbuch J., Linden T., Knieps E., Thömmes J., and Kula M.R. Dynamics of protein uptake within the adsorbent particle during packed bed chromatography. Biotechnology and Bioengineering 80 (2002) 359-368
13. Hunter A.K., and Carta G. Protein adsorption on novel acrylamido-based polymeric ion-exchangers. IV: effects of protein size on adsorption capacity and rate. Journal of Chromatography A 971 (2002) 105-116
14. Kaczmarski K., Cavazzini A., Szabelski P., Zhou D.M., Liu X., and Guiochon G. Application of the general rate model and the generalized Maxwell-Stefan equation to the study of the mass transfer kinetics of a pair of enantiomers. Journal of Chromatography A 962 (2002) 57-67
15. Kaczmarski K., Gubernak M., Zhou D., and Guiochon G. Application of the general rate model with the Maxwell-Stefan equations for the prediction of the band profiles of the 1-indanol enantiomers. Chemical Engineering Science 58 (2003) 2325-2338
16. Kapteijn F., Moulijn J.A., and Krishna R. The generalized Maxwell-Stefan model for diffusion in zeolites: sorbate molecules with different saturation loadings. Chemical Engineering Science 55 (2000) 2923-2930
17. Kasche V., de Boer M., Lazo C., and Gad M. Direct observation of intraparticle equilibration and the rate-limiting step in adsorption of proteins in chromatographic adsorbents with confocal laser scanning microscopy. Journal of Chromatography B 790 (2003) 115-129
18. Krishna R. Multicomponent surface-diffusion of adsorbed species-a description based on the generalized Maxwell-Stefan equations. Chemical Engineering Science 45 (1990) 1779-1791
19. Krishna R., and Wesselingh J.A. The Maxwell-Stefan approach to mass transfer. Chemical Engineering Science 52 (1997) 861-911
20. Lewus R.K., and Carta G. Binary protein adsorption on gel-composite ion-exchange media. A.I.Ch.E. Journal 45 (1999) 512-522
21. Linden T., Ljunglöf A., Hagel L., Kula M.R., and Thömmes J. Visualizing patterns of protein uptake to porous media using confocal scanning laser microscopy. Separation Science and Technology 37 (2002) 1-32
22. Ljunglöf A., and Hjorth R. Confocal microscopy as a tool for studying protein adsorption to chromatographic matrices. Journal Chromatography A 743 (1996) 75-83
23. Ljunglöf A., and Thömmes J. Visualising intraparticle protein transport in porous adsorbents by confocal microscopy. Journal Chromatography A 813 (1998) 387-395
24. Ljunglöf A., Laki K.M., Mueller J., Harinarayan C., van Reis R., Fahrner R., and Van Alstine J.M. Ion exchange chromatography of antibody fragments. Biotechnology and Bioengineering 96 (2007) 515-524
25. Ma Z., Whitley R.D., and Wang N.H.L. Pore and surface diffusion in multicomponent adsorption and liquid chromatography systems. A.I.Ch.E. Journal 42 (1996) 1244-1262
26. Maekawa M., Murakami K., and Yoshida H. Parallel diffusion of a sulfonated dye in cellulose membrane. Journal of Colloid and Interface Science 155 (1993) 79-84
27. Maekawa M., Kasai K., and Nango M. Transport phenomena of sulfonated dyes into cellulose membranes: parallel diffusion of a sulfonated dye with a high affinity onto cellulose. Colloids and Surfaces A: Physicochemical and Engineering Aspects 132 (1998) 173-179
28. Malmsten M., Xing K., and Ljunglöf A. Confocal microscopy studies of trypsin immobilization on porous glycidyl methacrylate beads. Journal of Colloid and Interface Science 220 (1999) 436-442
29. Martin C., Iberer G., Ubiera A., and Carta G. Two-component protein adsorption kinetics in porous ion exchange media. Journal Chromatography A 1079 (2005) 105-115
30. Mattisson C., Roger P., Jönsson B., Axelsson A., and Zacchi G. Diffusion of lysozyme in gels and liquids: a general approach for the determination of diffusion coefficients using holographic laser interferometry. Journal Chromatography B 743 (2000) 151-167
31. Pedersen H., Furler L., Venkatsubramanian K., Prenosil J., and Stuker E. Enzyme adsorption in porous supports: local thermodynamic equilibrium model. Biotechnology and Bioengineering 27 (1985) 961-971
32. Skidmore G.L., Horstmann B.J., and Chase H.A. Modelling single component adsorption to cation-exchangers. Journal of Chromatography 498 (1990) 113-128
33. Teske C.A., Schroeder M., Simon R., and Hubbuch J. Protein-labeling effect in confocal laser scanning microscopy. The Journal of Physical Chemistry B 109 (2005) 13811-13817
34. Teske C.A., Lieres E., Schroeder M., Ladiwala A., Cramer S.M., and Hubbuch J.J. Competitive adsorption of labeled and native protein in confocal laser scanning microscopy. Biotechnology and Bioengineering 95 (2006) 58-66
35. Tilton R.D., Robertson C.R., and Gast A.P. Lateral diffusion of bovine serum albumin adsorbed at the solid liquid interface. Journal of Colloid and Interface Science 137 (1990) 192-203
36. Wesselingh J.A., and Bosma J.C. Protein ion-exchange adsorption kinetics. A.I.Ch.E. Journal 47 (2001) 1571-1580
37. Wright P.R., Muzzio F.J., and Glasser B.J. Batch uptake of lysozyme: effect of solution viscosity and mass transfer on adsorption. Biotechnology Progress 14 (1998) 913-921
38. Xue B., and Sun Y. Protein adsorption equilibria and kinetics to a poly(vinyl alcohol)-based magnetic affinity support. Journal of Chromatography A 921 (2001) 109-119
39. Yang K., and Sun Y. Optics-intrinsic double-circle phenomenon in protein adsorption visualized by confocal laser scanning microscopy. Biochemical Engineering Journal 39 (2008) 258-266
40. Yang K., Shi Q.-H., and Sun Y. Modeling and simulation of protein uptake in cation exchanger visualized by confocal laser scanning microscopy. Journal of Chromatography A 1136 (2006) 19-28
41. Yoshida H., Maekawa M., and Nango M. Parallel transport by surface and pore diffusion in a porous membrane. Chemical Engineering Science 46 (1991) 429-438
42. Yoshida H., Yoshikawa M., and Kataoka T. Parallel transport of BSA by surface and pore diffusion in strongly basic chitosan. A.I.Ch.E. Journal 40 (1994) 2034-2044
43. Zhang X., Wang J.-C., Lacki K.M., and Liapis A.I. Molecular dynamics simulation of the transport and adsorption of a charged macromolecule onto a charged adsorbent solid surface immersed in an electrolytic solution. Journal of Colloid and Interface Science 277 (2004) 483-498
44. Zhang X., Wang J.-C., Lacki K.M., and Liapis A.I. Molecular dynamics simulation studies of the conformation and lateral mobility of a charged adsorbate biomolecule: implications for estimating the critical value of a pore in porous media. Journal of Colloid and Interface Science 290 (2005) 373-382
45. Zhou X.P., Li W., Shi Q.H., and Sun Y. Analysis of mass transport models for protein adsorption to cation exchanger by visualization with confocal laser scanning microscopy. Journal of Chromatography A 1103 (2006) 110-117