Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports

Journal of Chromatography A

Volumn 848, Issue 1-2, 1999, Pages 61-70

  Armisén, Pilar ^a   Mateo, Cesar ^b   Cortés, Estrella ^c   Barredo, José L ^d   Salto, Francisco ^d   Diez, Bruno ^d   Rodés, Lorenzo ^e   García, José L ^c   Fernández Lafuente, Roberto ^b   Guisán, José M ^b  

^a Hispanagar SA   (Spain)

^b INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^c CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^d Avenida de Antibioóticos 59 61   (Spain)

^e CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Affinity supports; Enzymes; Glutaryl acylase; Immobilized metal ion affinity chromatography

Indexed keywords

AMIDASE; METAL CHELATE;

ACINETOBACTER; ADSORPTION; AFFINITY CHROMATOGRAPHY; ARTICLE; ESCHERICHIA COLI; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ACINETOBACTER; AMIDOHYDROLASES; BASE SEQUENCE; CHELATING AGENTS; CHROMATOGRAPHY, AFFINITY; COPPER; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HISTIDINE; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; PENICILLIN AMIDASE; RECOMBINANT PROTEINS;

ACINETOBACTER SP.;

EID: 0032982259     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-9673(99)00489-6     Document Type: Article

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