UvrD2 is essential in Mycobacterium tuberculosis, but its helicase activity is not required

Journal of Bacteriology

Volumn 193, Issue 17, 2011, Pages 4487-4494

  Williams, Alan ^a   Güthlein, Carolin ^b   Beresford, Nicola ^a   Böttger, Erik C ^b   Springer, Burkhard ^c   Davis, Elaine O ^a  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c AUSTRIAN AGENCY FOR HEALTH AND FOOD SAFETY   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL DNA; HELICASE; UNCLASSIFIED DRUG; UVRD2 PROTEIN;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE DELETION; GENE INACTIVATION; GENE LOCUS; GENE TRANSLOCATION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CHROMOSOMES, BACTERIAL; DNA HELICASES; DNA, BACTERIAL; ESCHERICHIA COLI; GENE DELETION; GENES, BACTERIAL; GENES, ESSENTIAL; GENETIC LOCI; HYDROLYSIS; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM TUBERCULOSIS; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; TRANSLOCATION, GENETIC;

MYCOBACTERIUM TUBERCULOSIS;

EID: 80052315960     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00302-11     Document Type: Article

References (38)

1. Anand, S. P., H. Zheng, P. R. Bianco, S. H. Leuba, and S. A. Khan. 2007. DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated strand exchange. J. Bacteriol. 189:4502-4509.
2. Bruand, C., and S. D. Ehrlich. 2000. UvrD-dependent replication of rollingcircle plasmids in Escherichia coli. Mol. Microbiol. 35:204-210.
3. Cole, S. T., et al. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544.
4. Curti, E., S. J. Smerdon, and E. O. Davis. 2007. Characterization of the helicase activity and substrate specificity of Mycobacterium tuberculosis UvrD. J. Bacteriol. 189:1542-1555.
5. Darwin, K. H., and C. F. Nathan. 2005. Role for nucleotide excision repair in virulence of Mycobacterium tuberculosis. Infect. Immun. 73:4581-4587.
6. Davis, E. O., et al. 2002. DNA damage induction of recA in Mycobacterium tuberculosis independently of RecA and LexA. Mol. Microbiol. 46:791-800.
7. Dillingham, M. S., P. Soultanas, and D. B. Wigley. 1999. Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation. Nucleic Acids Res. 27:3310-3317.
8. Dutta, N. K., et al. Genetic requirements for the survival of tubercle bacilli in primates. J. Infect. Dis. 201:1743-1752.
9. Flores, M. J., N. Sanchez, and B. Michel. 2005. A fork-clearing role for UvrD. Mol. Microbiol. 57:1664-1675.
10. Guthlein, C., et al. 2009. Characterization of the mycobacterial NER system reveals novel functions of the uvrD1 helicase. J. Bacteriol. 191:555-562.
11. Guy, C. P., et al. 2009. Rep provides a second motor at the replisome to promote duplication of protein-bound DNA. Mol. Cell 36:654-666.
12. Lamichhane, G., et al. 2003. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. U. S. A. 100:7213-7218.
13. Lee, M. H., L. Pascopella, W. R. Jacobs, Jr., and G. F. Hatfull. 1991. Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin. Proc. Natl. Acad. Sci. U. S. A. 88:3111-3115.
14. Lestini, R., and B. Michel. 2008. UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli. J. Bacteriol. 190: 5995-6001.
15. Lestini, R., and B. Michel. 2007. UvrD controls the access of recombination proteins to blocked replication forks. EMBO J. 26:3804-3814.
16. Matson, S. W., and A. B. Robertson. 2006. The UvrD helicase and its modulation by the mismatch repair protein MutL. Nucleic Acids Res. 34: 4089-4097.
17. Mendonca, V. M., and S. W. Matson. 1995. Genetic analysis of delta helD and delta uvrD mutations in combination with other genes in the RecF recombination pathway in Escherichia coli: suppression of a ruvB mutation by a uvrD deletion. Genetics 141:443-452.
18. Moolenaar, G. F., C. Moorman, and N. Goosen. 2000. Role of the Escherichia coli nucleotide excision repair proteins in DNA replication. J. Bacteriol. 182:5706-5714.
19. Murray, H. W., C. W. Juangbhanich, C. F. Nathan, and Z. A. Cohn. 1979. Macrophage oxygen-dependent antimicrobial activity. II. The role of oxygen intermediates. J. Exp. Med. 150:950-964.
20. Pashley, C. A., and T. Parish. 2003. Efficient switching of mycobacteriophage L5-based integrating plasmids in Mycobacterium tuberculosis. FEMS Microbiol. Lett. 229:211-215.
21. Pena, C. E., J. M. Kahlenberg, and G. F. Hatfull. 1999. Protein-DNA complexes in mycobacteriophage L5 integrative recombination. J. Bacteriol. 181: 454-461.
22. Pena, C. E., M. H. Lee, M. L. Pedulla, and G. F. Hatfull. 1997. Characterization of the mycobacteriophage L5 attachment site, attP. J. Mol. Biol. 266:76-92.
23. Richards, J. D., et al. 2008. Structure of the DNA repair helicase hel308 reveals DNA binding and autoinhibitory domains. J. Biol. Chem. 283:5118- 5126.
24. Rickman, L., et al. 2005. A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor. Mol. Microbiol. 56:1274-1286.
25. Sambrook, J., E. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
26. +: a dominant selectable marker for gene replacement in mycobacteria. Mol. Microbiol. 16:991-1000.
27. Sassetti, C. M., D. H. Boyd, and E. J. Rubin. 2003. Genes required for mycobacterial growth defined by high density mutagenesis. Mol. Microbiol. 48:77-84.
28. Singh, P., et al. Mycobacterium tuberculosis UvrD1 and UvrA proteins suppress DNA strand exchange promoted by cognate and noncognate RecA proteins. Biochemistry 49:4872-4883.
29. Sinha, K. M., M. S. Glickman, and S. Shuman. 2009. Mutational analysis of Mycobacterium UvrD1 identifies functional groups required for ATP hydrolysis, DNA unwinding, and chemomechanical coupling. Biochemistry 48: 4019-4030.
30. Sinha, K. M., N. C. Stephanou, F. Gao, M. S. Glickman, and S. Shuman. 2007. Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair. J. Biol. Chem. 282:15114-15125.
31. Sinha, K. M., N. C. Stephanou, M. C. Unciuleac, M. S. Glickman, and S. Shuman. 2008. Domain requirements for DNA unwinding by mycobacterial UvrD2, an essential DNA helicase. Biochemistry 47:9355-9364.
32. Springer, B., P. Sander, L. Sedlacek, K. Ellrott, and E. C. Bottger. 2001. Instability and site-specific excision of integration-proficient mycobacteriophage L5 plasmids: development of stably maintained integrative vectors. Int. J. Med. Microbiol. 290:669-675.
33. Stover, C. K., et al. 1991. New use of BCG for recombinant vaccines. Nature 351:456-460.
34. Stuehr, D. J., and M. A. Marletta. 1987. Synthesis of nitrite and nitrate in murine macrophage cell lines. Cancer Res. 47:5590-5594.
35. Wanner, R. M., et al. 2009. The uracil DNA glycosylase UdgB of Mycobacterium smegmatis protects the organism from the mutagenic effects of cytosine and adenine deamination. J. Bacteriol. 191:6312-6319.
36. Wanner, R. M., C. Guthlein, B. Springer, E. C. Bottger, and M. Ackermann. 2008. Stabilization of the genome of the mismatch repair deficient Mycobacterium tuberculosis by context-dependent codon choice. BMC Genomics 9:249.
37. Wards, B. J., and D. M. Collins. 1996. Electroporation at elevated temperatures substantially improves transformation efficiency of slow-growing mycobacteria. FEMS Microbiol. Lett. 145:101-105.
38. WHO. 2009. Tuberculosis facts 2009 update. World Health Organization, Geneva, Switzerland.