Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation

Nucleic Acids Research

Volumn 27, Issue 16, 1999, Pages 3310-3317

  Dillingham, Mark S ^a   Soultanas, Panos ^a   Wigley, Dale B ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; HELICASE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA BINDING; DRUG HYDROLYSIS; ELECTROPHORETIC MOBILITY; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME MECHANISM; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN FAMILY; SITE DIRECTED MUTAGENESIS;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEINS; DNA HELICASES; DNA, SINGLE-STRANDED; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED;

EID: 0033566657     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/27.16.3310     Document Type: Article

References (24)

1. Lohman,T.M. and Bjornson,K.P. (1996) Annu. Rev. Biochem., 65, 169-214.
2. Bird,L.E., Subramanya,H.S. and Wigley,D.B. (1998) Curr. Opin. Struct. Biol., 8, 14-18.
3. Wong,I. and Lohman,T.M. (1992) Science, 256, 350-355.
4. Iordanescu,S. (1993) Mol. Gen. Genet., 241, 185-192.
5. Bird,L.E., Brannigan,J.A., Subramanya,H.S. and Wigley,D.B. (1998) Nucleic Acids Res., 26, 2686-2693.
6. Subramanya,H.S., Bird,L.E., Brannigan,J.A. and Wigley,D.B. (1996) Nature, 384, 379-383.
7. Velankar,S., Soultanas,P., Dillingham,M.S., Subramanya,H.S. and Wigley,D.B. (1999) Cell, 97, 75-84.
8. Brosh,R.M.Jr and Matson,S.W. (1996) J. Biol. Chem., 271, 25360-25368.
9. Brosh,R.M.Jr and Matson,S.W. (1997) J. Biol. Chem., 272, 572-579.
10. Korolev,S., Hsieh,J., Gauss,G.H., Lohman,T.M. and Waksman,G. (1997) Cell, 90, 635-647.
11. Story,R.M. and Steitz,T.A. (1992) Nature, 355, 374-376.
12. Milburn,M.V., Tong,L., DeVos,A.M., Brünger,A., Yamaizumi,Z., Nishimura,S. and Kim,S.-H. (1990) Science, 247, 939-945.
13. Kelley,J.A. and Knight,K.L. (1997) J. Biol. Chem., 272, 25778-25782.
14. Clackson,T., Güssow,D. and Jones,P.T. (1991) In McPherson,M.J., Quirke,P. and Taylor,G.R. (eds) PCR - A Practical Approach. IRL Press, Oxford. UK, pp. 207-209.
15. Soultanas,P., Dillingham,M.S. and Wigley.D.B. (1998) Nucleic Acids Res., 26, 2374-2379.
16. Pullman,M.E., Penefsky,H.S., Datta,A. and Racker,E. (1960) J. Biol. Chem., 235, 3322-3329.
17. Crute,J.J., Mocarski,E.S. and Lehman,I.R. (1988) Nucleic Acids Res., 16, 6585-6596.
18. Bjornson,K.P., Moore,K.J.M. and Lohman,T.M. (1996) Biochemistry, 35, 2268-2282.
19. Malkov,V.A. and Camerini-Otero,R.D. (1995) J. Biol. Chem., 270, 30230-30233.
20. Voloshin,O.N., Wang,L. and Camerini-Otero,R.D. (1996) Science, 272, 868-872.
21. Hall,M.C., Özsoy,A.Z. and Matson,S.W. (1998) J. Mol. Biol., 277, 257-271.
22. Soultanas,P., Dillingham,M.S., Velankar,S.S. and Wigley,D.B. (1999) J. Mol. Biol., 290, 137-148.
23. Berchtold,H., Reshetnikova,L., Reiser,C.O.A., Schirmer,N.K., Sprinzl,M. and Hilgenfeld,R. (1993) Nature, 365, 126-132.
24. Bourne,H.R., Sanders,D.A. and McCormick,F. (1991) Nature, 349, 117-127.