Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen

Molecular Systems Biology

Volumn 7, Issue , 2011, Pages

  Saeidi, Nazanin ^a   Wong, Choon Kit ^a   Lo, Tat Ming ^a   Nguyen, Hung Xuan ^a   Ling, Hua ^a   Leong, Susanna Su Jan ^a   Poh, Chueh Loo ^a   Chang, Matthew Wook ^a  

^a Nanyang Technological University   (Singapore)

Author keywords

genetic circuits; Pseudomonas aeruginosa; pyocin; quorum sensing; synthetic biology

Indexed keywords

PYOCIN; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; DRUG DERIVATIVE; GAMMA BUTYROLACTONE; GREEN FLUORESCENT PROTEIN; HOMOSERINE; HYBRID PROTEIN; N (3 OXODODECANOYL)HOMOSERINE LACTONE; N-(3-OXODODECANOYL)HOMOSERINE LACTONE;

ARTICLE; BACTERIAL STRAIN; CELL VIABILITY; CONTROLLED STUDY; ESCHERICHIA COLI; GENETIC ENGINEERING AND GENE TECHNOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECRETION; PSEUDOMONAS AERUGINOSA; QUORUM SENSING; ANTIBIOSIS; BIOFILM; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETIC PROCEDURES; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; METHODOLOGY; MICROBIOLOGY; PATHOGENICITY; PLASMID; PSEUDOMONAS INFECTION; REPORTER GENE; SYNTHETIC BIOLOGY; TRANSGENIC ORGANISM;

ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA;

4-BUTYROLACTONE; ANTI-BACTERIAL AGENTS; ANTIBIOSIS; BACTERIAL PROTEINS; BIOFILMS; BIOSENSING TECHNIQUES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HOMOSERINE; HUMANS; ORGANISMS, GENETICALLY MODIFIED; PLASMIDS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; PYOCINS; QUORUM SENSING; RECOMBINANT FUSION PROTEINS; SYNTHETIC BIOLOGY;

EID: 80051910411     PISSN: None     EISSN: 17444292     Source Type: Journal    
DOI: 10.1038/msb.2011.55     Document Type: Article

References (31)

1. Anderson JC, Clarke EJ, Arkin AP, Voigt CA (2006) Environmentally controlled invasion of cancer cells by engineered bacteria. J Mol Biol 355: 619-627 (Pubitemid 41817623)
2. Asad S, Opal SM (2008) Bench-to-bedside review: quorum sensing and the role of cell-to-cell communication during invasive bacterial infection. Crit Care 12: 236
3. Baba T, Schneewind O (1998) Instruments of microbial warfare: bacteriocin synthesis, toxicity and immunity. Trends Microbiol 6: 66-71 (Pubitemid 28080754)
4. BradfordMM(1976) A rapid and sensitive method for the quantitation of microgramquantities of protein utilizing the principle of proteindye binding. Anal Biochem 72: 248-254
5. Brook I (2005) The role of bacterial interference in otitis, sinusitis and tonsillitis. Otolaryngol Head Neck Surg 133: 139-146 (Pubitemid 40980976)
6. Canton B, Labno A, Endy D (2008) Refinement and standardization of synthetic biological parts and devices. Nat Biotechnol 26: 787-793 (Pubitemid 351961452)
7. Chak KF, Kuo WS, Lu FM, James R (1991) Cloning and characterization of the ColE7 plasmid. J Gen Microbiol 137: 91-100
8. Chang W, Small DA, Toghrol F, Bentley WE (2005a) Microarray analysis of Pseudomonas aeruginosa reveals induction of pyocin genes in response to hydrogen peroxide. BMC Genomics 6: 115
9. Chang W, Small DA, Toghrol F, Bentley WE (2005b) Microarray analysis of toxicogenomic effects of peracetic acid on Pseudomonas aeruginosa. Environ Sci Technol 39: 5893-5899 (Pubitemid 41098429)
10. Charlson ES, Chen J, Custers-Allen R, Bittinger K, Li H, Sinha R, Hwang J, Bushman FD, Collman RG (2010) Disordered microbial communities in the upper respiratory tract of cigarette smokers. PLoS ONE 5: e15216
11. Charlton TS, de Nys R, Netting A, Kumar N, Hentzer M, Givskov M, Kjelleberg S (2000) A novel and sensitive method for the quantification of N-3-oxoacyl homoserine lactones using gas chromatography-mass spectrometry: application to a model bacterial biofilm. Environ Microbiol 2: 530-541 (Pubitemid 33136691)
12. Chen Y, Leong SS (2009) Adsorptive refolding of a highly disulfidebonded inclusion body protein using anion-exchange chromatography. J Chromatogr A 1216: 4877-4886
13. Choi K-H, Kumar A, Schweizer HP (2006) A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J Microbiol Methods 64: 391-397 (Pubitemid 43212814)
14. Endy D (2005) Foundations for engineering biology. Nature 438: 449-453 (Pubitemid 41742010)
15. Fujitani S, Sun H-Y, Yu VL, Weingarten JA (2011) Pneumonia due to Pseudomonas aeruginosa. Chest 139: 909-919
16. Gray KM, Passador L, Iglewski BH, Greenberg EP (1994) Interchangeability and specificity of components from the quorum-sensing regulatory systems of Vibrio fischeri and Pseudomonas aeruginosa. J Bacteriol 176: 3076-3080 (Pubitemid 24152957)
17. Häusler T (2006) Viruses vs. Superbugs: A Solution to the Antibiotics Crisis? MacMillan, New York, NY, USA
18. Huang L, Leong SS, Jiang R (2009) Soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27. Appl Microbiol Biotechnol 84: 301-308
19. Lin LJRL, Chen CL, Chen YR, Chak KF (2009) Induction of membrane permeability in Escherichia colimediated by lysis protein of the ColE7 operon. FEMS Microbiol Lett 298: 85-92
20. Ling H, Saeidi N, Rasouliha BH, Chang MW (2010) A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage. FEBS Lett 584: 3354-3358
21. Pearson JP, Passador L, Iglewski BH, Greenberg EP (1995) A second Nacylhomoserine lactone signal produced by Pseudomonas aeruginosa. Proc Natl Acad Sci USA 92: 1490-1494
22. Ro DK, Paradise EM, Ouellet M, Fisher KJ, Newman KL, Ndungu JM, Ho KA, Eachus RA, Ham TS, Kirby J, Chang MC, Withers ST, Shiba Y, Sarpong R, Keasling JD (2006) Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 440: 940-943
23. Scholl D, Martin Jr DW (2008) Antibacterial efficacy of R-Type pyocins towards pseudomonas aeruginosa in a Murine Peritonitis Model. Antimicrob Agents Chemother 52: 1647-1652 (Pubitemid 351614656)
24. Schuster M, UMLGEP (2004) Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR. Proc Natl Acad Sci USA 101: 15833-15839 (Pubitemid 39507888)
25. Seo Y, Galloway DR (1990) Purification of the pyocin S2 complex from Pseudomonas aeruginosa PAO1: analysis of DNase activity. Biochem Biophys Res Commun 172: 455-461 (Pubitemid 20384442)
26. Sinha J, Reyes SJ, Gallivan JP (2010) Reprogramming bacteria to seek and destroy an herbicide. Nat Chem Biol 6: 464-470
27. Small DA, ChangW, Toghrol F, BentleyWE (2007) Comparative global transcription analysis of sodium hypochlorite, peracetic acid, and hydrogen peroxide on Pseudomonas aeruginosa. Appl Microbiol Biotechnol 76: 1093-1105 (Pubitemid 47415606)
28. Smith AW, Hirst PH, Hughes K, Gensberg K, Govan JR (1992) The pyocin Sa receptor of Pseudomonas aeruginosa is associated with ferripyoverdin uptake. J Bacteriol 174: 4847-4849
29. Smith C, Song H, You L (2008) Signal discrimination by differential regulation of protein stability in quorum sensing. J Mol Biol 382: 1290-1297
30. Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del Cardayre SB, Keasling JD (2010) Microbial production of fattyacid- derived fuels and chemicals from plant biomass. Nature 463: 559-562
31. Wright A, Hawkins CH, Ä nggård EE, Harper DR (2009) A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa; a preliminary report of efficacy. Clin Otolaryngol 34: 349-357