Application of Halotag protein to covalent immobilization of recombinant proteins for single molecule force spectroscopy

Langmuir

Volumn 26, Issue 13, 2010, Pages 10433-10436

  Taniguchi, Yukinori ^a   Kawakami, Masaru ^a  

^a JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ATOMIC FORCE MICROSCOPES; CONTOUR LENGTHS; COVALENT IMMOBILIZATION; FORCE-EXTENSION; MECHANICAL UNFOLDING; MICA SUBSTRATES; MICA SURFACES; POLYPROTEINS; RECOMBINANT PROTEIN; SINGLE MOLECULE FORCE SPECTROSCOPY; SITE-SPECIFIC; STANDARD METHOD;

ATOMIC SPECTROSCOPY; MICA; MOLECULAR PHYSICS; SILICATE MINERALS;

PROTEINS;

HALOALKANE DEHALOGENASE; HYBRID PROTEIN; HYDROLASE;

ARTICLE; ATOMIC FORCE MICROSCOPY; CHEMISTRY; GENETICS; METABOLISM; METHODOLOGY; THEORETICAL MODEL;

HYDROLASES; MICROSCOPY, ATOMIC FORCE; MODELS, THEORETICAL; RECOMBINANT FUSION PROTEINS;

EID: 77954268327     PISSN: 07437463     EISSN: 15205827     Source Type: Journal    
DOI: 10.1021/la101658a     Document Type: Article

References (28)

1. Carrion-Vazquez, M., Oberhauser, A. F., Fowler, S. B., Marszalek, P. E., Broedel, S. E., Clarke, J., and Fernandez, J. M. Mechanical and chemical unfolding of a single protein: a comparison. Proc. Natl. Acad. Sci. U.S.A. 1999, 96 (7), 3694 - 3699.
2. Brockwell, D. J. Probing the mechanical stability of proteins using the atomic force microscope. Biochem. Soc. Trans. 2007, 35 (Pt 6), 1564 - 1568.
3. Grandbois, M., Beyer, M., Rief, M., Clausen-Schaumann, H., and Gaub, H. E. How strong is a covalent bond? Science 1999, 283 (5408), 1727 - 1730.
4. Brockwell, D. J., Beddard, G. S., Clarkson, J., Zinober, R. C., Blake, A. W., Trinick, J., Olmsted, P. D., Smith, D. A., and Radford, S. E. The effect of core destabilization on the mechanical resistance of I27. Biophys. J. 2002, 83 (1), 458 - 472.
5. Rainwater, R., Parks, D., Anderson, M. E., Tegtmeyer, P., and Mann, K. Role of cysteine residues in regulation of p53 function. Mol. Cell. Biol. 1995, 15 (7), 3892 - 3903.
6. Nicholson, D. W. and Thornberry, N. A. Caspases: killer proteases. Trends Biochem. Sci. 1997, 22 (8), 299 - 306.
7. Kienberger, F., Kada, G., Gruber, H. J., Pastushenko, V. P., Riener, C., Trieb, M., Knaus, H.-G., Schindler, H., and Hinterdorfer, P. Recognition force spectroscopy studies of the NTA-His6 bond. Single Mol. 2000, 1 (1), 59 - 65.
8. Verbelen, C., Gruber, H. J., and Dufrene, Y. F. The NTA-His6 bond is strong enough for AFM single-molecular recognition studies. J. Mol. Recognit. 2007, 20 (6), 490 - 494.
9. Yoshimura, S. H., Takahashi, H., Otsuka, S., and Takeyasu, K. Development of glutathione-coupled cantilever for the single-molecule force measurement by scanning force microscopy. FEBS Lett. 2006, 580 (16), 3961 - 3965.
10. Florin, E. L., Moy, V. T., and Gaub, H. E. Adhesion forces between individual ligand-receptor pairs. Science 1994, 264 (5157), 415 - 417.
11. Moy, V. T., Florin, E. L., and Gaub, H. E. Intermolecular forces and energies between ligands and receptors. Science 1994, 266 (5183), 257 - 259.
12. Kufer, S. K., Dietz, H., Albrecht, C., Blank, K., Kardinal, A., Rief, M., and Gaub, H. E. Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy. Eur. Biophys. J. 2005, 35 (1), 72 - 78.
13. Brockwell, D. J., Paci, E., Zinober, R. C., Beddard, G. S., Olmsted, P. D., Smith, D. A., Perham, R. N., and Radford, S. E. Pulling geometry defines the mechanical resistance of a beta-sheet protein. Nat. Struct. Biol. 2003, 10 (9), 731 - 737.
14. Carrion-Vazquez, M., Li, H., Lu, H., Marszalek, P. E., Oberhauser, A. F., and Fernandez, J. M. The mechanical stability of ubiquitin is linkage dependent. Nat. Struct. Biol. 2003, 10 (9), 738 - 743.
15. Los, G. V. and Wood, K. The HaloTag: a novel technology for cell imaging and protein analysis. Methods Mol. Biol. 2007, 356, 195 - 208.
16. Ohana, R. F., Encell, L. P., Zhao, K., Simpson, D., Slater, M. R., Urh, M., and Wood, K. V. HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification. Protein Expression Purif. 2009, 68 (1), 110 - 120.
17. Zinober, R. C., Brockwell, D. J., Beddard, G. S., Blake, A. W., Olmsted, P. D., Radford, S. E., and Smith, D. A. Mechanically unfolding proteins: the effect of unfolding history and the supramolecular scaffold. Protein Sci. 2002, 11 (12), 2759 - 2765.
18. Wang, H., Bash, R., Yodh, J. G., Hager, G. L., Lohr, D., and Lindsay, S. M. Glutaraldehyde modified mica: a new surface for atomic force microscopy of chromatin. Biophys. J. 2002, 83 (6), 3619 - 3625.
19. Butt, H. J. and Jaschke, M. Calculation of thermal noise in atomic force microscopy. Nanotechnology 1995, 6, 1 - 7.
20. Florin, E. L., Rief, M., Lehmann, H., Ludwig, M., Dornmair, C., Moy, V. T., and Gaub, H. E. Sensing specific molecular interactions with the atomic force microscope. Biosens. Bioelectron. 1995, 10, 895 - 901.
21. Marko, J. F. and Siggia, E. D. Stretching DNA. Macromolecules 1995, 28, 8759 - 8770.
22. Taniguchi, Y., Brockwell, D. J., and Kawakami, M. The effect of temperature on mechanical resistance of the native and intermediate States of I27. Biophys. J. 2008, 95, 5296 - 5305.
23. Schwaiger, I., Kardinal, A., Schleicher, M., Noegel, A. A., and Rief, M. A mechanical unfolding intermediate in an actin-crosslinking protein. Nat. Struct. Mol. Biol. 2004, 11 (1), 81 - 85.
24. Bertz, M. and Rief, M. Mechanical unfoldons as building blocks of maltose-binding protein. J. Mol. Biol. 2008, 378 (2), 447 - 458.
25. Peng, Q. and Li, H. Atomic force microscopy reveals parallel mechanical unfolding pathways of T4 lysozyme: evidence for a kinetic partitioning mechanism. Proc. Natl. Acad. Sci. U.S.A. 2008, 105 (6), 1885 - 1890.
26. Janssen, D. B. Evolving haloalkane dehalogenases. Curr. Opin. Chem. Biol. 2004, 8 (2), 150 - 159.
27. Newman, J., Peat, T. S., Richard, R., Kan, L., Swanson, P. E., Affholter, J. A., Holmes, I. H., Schindler, J. F., Unkefer, C. J., and Terwilliger, T. C. Haloalkane dehalogenases: structure of a Rhodococcus enzyme. Biochemistry 1999, 38 (49), 16105 - 16114.
28. Wang, T., Arakawa, H., and Ikai, A. Force measurement and inhibitor binding assay of monomer and engineered dimer of bovine carbonic anhydrase B. Biochem. Biophys. Res. Commun. 2001, 285 (1), 9 - 14.