An efficient method for stable transfection of mouse myogenic C2C12 cell line using a nonviral transfection approach;C2C12 fare miyoblast hücre hattında viral olmayan transfeksiyon yöntemi ile kal1c1 transfeksiyonun gerçekleştirilmesi

Turkish Journal of Medical Sciences

Volumn 41, Issue 5, 2011, Pages 821-825

  Akyüz, Mehmet Deniz ^a   Balci Hayta, Burcu ^a   Dinçer, Pervin R ^a  

^a HACETTEPE UNIVERSITY   (Turkey)

Author keywords

C2C12 cell line; Inducible expression; Stable transfection; Tetracycline resistance gene

Indexed keywords

GREEN FLUORESCENT PROTEIN; TETRACYCLINE; TETRACYCLINE RESISTANCE PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL CULTURE; CELL LINE; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENE FUNCTION; GENETIC TRANSFECTION; MOLECULAR CLONING; MOUSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PLASMID; PROTEIN EXPRESSION; REPORTER GENE;

EID: 80051718072     PISSN: 13000144     EISSN: None     Source Type: Journal    
DOI: 10.3906/sag-1008-1057     Document Type: Article

References (20)

1. Ramnarain DB, Park S, Lee DY, Hatanpaa KJ,Scoggin SO,Out H et al. Differential gene expression analysis reveals generation of an autocrine loop by a mutant epidermal growth factor receptor in glioma cells.Cancer Res2006; 66: 867-874
2. Tao J, Wendler P, Connelly G, Lim A, Zhang J, King M et al. Drug target validation: lethal infection blocked by inducible peptide.Proc Natl Acad Sci USA 2000; 2: 783-786
3. Miyagawa Y,Okita H, Nakaijima H,Horiuchi Y,Sato B,Taguchi T et al. Inducible expression of chimeric EWS/ETS proteins confers Ewing's family tumor-like phenotypes to human mesenchymal progenitor cells. Mol Cell Biol 2008; 28: 2125-2137
4. Southan A, Maidment S, Dowler S, Southan AP, Maidment S, Dowle S et al. Using inducible expression vector technology to create stable cell lines expressing KCNQ2/3, KCNQ4, and KCNQ3/5 currents suitable for automated electrophysiology platforms. Biophys J 2009; 96: 177a
5. Baron U, Bujard H. Tet repressor-based system for regulated gene expression in eukaryotic cells: Principles and advances. Methods Enzymol 2000; 327: 401-421
6. Lewandoski M. Conditional control of gene expression in the mouse. Nat Rev Genet 2001; 2: 743-755
7. Gossen M, Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 1992; 89: 5547-5551
8. Hillen W, Berens C. Mechanisms underlying expression of Tn10 encoded tetracycline resistance. Annu Rev Microbiol 1994; 48: 345-369
9. Hillen W, Gatz C, Altschmied L, Schollmeier K, Meier I. Control of expression of the Tn10-encoded tetracycline resistance genes: Equilibrium and kinetic investigations of the regulatory reactions. J Mol Biol 1983; 169: 707-721
10. Yao F, Svensjö T, Winkler T, Lu M, Eriksson C, Eriksson E. Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells. Hum Gene Ther 1998; 9: 1939-1950
11. Dobrovolsky VN, McGarrity LJ, Morris SM, Heflich RH. Detection of mutation in transgenic CHO cells using green fluorescent protein as a reporter. Mutat Res 2002; 518: 55-64
12. Jones J, Nivitchanyong T, Giblin C, Ciccarone V, Judd D, Gorfien S et al. Optimization of tetracycline-responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells. Biotechnol Bioeng 2005; 91: 722-732
13. RauthS, Kucherlapati RS. Expression of DNA transferred into mammalian cells. J Biosci 1984;6: 543-567
14. Wallenburg JC, Chartrand P. Inducible expression vectors for gene therapy using bone marrow. Transfus Sci 1996; 17: 155-168
15. Rossi FMV, Blau HM. Recent advances in inducible gene expression systems. Curr Opin Biotechnol 1998; 9: 451-456
16. Reeves PJ, Callewaert N, Contreras R, Khorana HG. Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyl transferase I-negative HEK293S stable mammalian cell line. Proc Natl Acad Sci USA2002; 99: 13419-13424
17. Song P, Wang HCR. Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression. Electron J Biotechnol 2004; 7: 210-213
18. Amar L, Desclaux M, Faucon-Biguet N, Mallet J, Vogel R. Control of small inhibitory RNA levels and RNA interference by doxycycline induced activation of a minimal RNA polymerase III promoter. Nucleic Acids Res 2006; 34: e37
19. Craig NL. The mechanism of conservative site-specific recombination. Ann Rev Genet 1988; 22: 77-105
20. Sauer B. Site-specific recombination: developments and applications. Curr Opin Biotechnol 1994; 5: 521-527