Engineering [Ln(DPA) 3] 3- binding sites in proteins: A widely applicable method for tagging proteins with lanthanide ions

Journal of Biomolecular NMR

Volumn 50, Issue 4, 2011, Pages 411-420

  Jia, Xinying ^a   Yagi, Hiromasa ^a   Su, Xun Cheng ^a   Stanton Cook, Mitchell ^a   Huber, Thomas ^a   Otting, Gottfried ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

Ln(DPA) 3 3 ; Lanthanide tag; NMR spectroscopy; Paramagnetic relaxation enhancements; RRM domain

Indexed keywords

AMINO ACID; ARGININE; DIPICOLINIC ACID; HISTIDINE; LANTHANIDE; MUTANT PROTEIN; NITROGEN 15;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; COMPLEX FORMATION; DISULFIDE BOND; ISOTOPE LABELING; METHODOLOGY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PLASMID; PRIORITY JOURNAL; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS; SPECTROMETER;

ANIMALS; BINDING SITES; ESCHERICHIA COLI PROTEINS; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEINS; ISOTOPE LABELING; LANTHANOID SERIES ELEMENTS; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PICOLINIC ACIDS; PROTEINS;

EID: 80051684792     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-011-9529-x     Document Type: Article

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