Glutarate and N-acetyl-L-glutamate buffers for cell-free synthesis of selectively 15N-labelled proteins

Journal of Biomolecular NMR

Volumn 44, Issue 2, 2009, Pages 59-67

  Jia, Xinying ^a   Ozawa, Kiyoshi ^a   Loscha, Karin ^a   Otting, Gottfried ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

15N HSQC; Cell free protein synthesis; Combinatorial15N labelling; Potassium glutarate; Potassium N acetyl L glutamate; Selective 15N Glu labelling

Indexed keywords

2 OXOGLUTARIC ACID; 4 AMINOBUTYRIC ACID; ADIPIC ACID; AMINO ACID; AMINOTRANSFERASE; ARGININE; BINDING PROTEIN; BUFFER; CYCLOPHILIN A; DIGLYCINE; DNA DIRECTED DNA POLYMERASE GAMMA; GLUTAMIC ACID; GLUTARIC ACID; GLYCOLIC ACID; ISOPHTHALIC ACID; MALONIC ACID; N ACETYLGLUTAMIC ACID; NITROGEN 15; PEPTIDYLPROLYL ISOMERASE; PHTHALIC ACID; PROTEINASE; SUCCINIC ACID; TARTARIC ACID; TEREPHTHALIC ACID;

ARTICLE; CELL FREE SYSTEM; CHEMICAL STRUCTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; HETERONUCLEAR SINGLE QUANTUM COHERENCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN SYNTHESIS;

AMINO ACIDS; BUFFERS; CELL-FREE SYSTEM; GLUTAMATES; GLUTARATES; ISOTOPE LABELING; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEINS; TRANSAMINASES;

EID: 67349257891     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-009-9315-1     Document Type: Article

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